Publications by authors named "Lucas Buscaratti"
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the agent of a major global outbreak of respiratory tract disease known as Coronavirus Disease 2019 (COVID-19). SARS-CoV-2 infects mainly lungs and may cause several immune-related complications, such as lymphocytopenia and cytokine storm, which are associated with the severity of the disease and predict mortality. The mechanism by which SARS-CoV-2 infection may result in immune system dysfunction is still not fully understood.
View Article and Find Full Text PDFThe COVID-19 (Coronavirus Disease 2019), caused by the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), severely affects mainly individuals with pre-existing comorbidities. Here our aim was to correlate the mTOR (mammalian/mechanistic Target of Rapamycin) and autophagy pathways with the disease severity. Through western blotting and RNA analysis, we found increased mTOR signaling and suppression of genes related to autophagy, lysosome, and vesicle fusion in Vero E6 cells infected with SARS-CoV-2 as well as in transcriptomic data mining of bronchoalveolar epithelial cells from severe COVID-19 patients.
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- Hypothalamic interleukin-6 (IL6) activates the ERK1/2 pathway in the ventromedial hypothalamus, which promotes fatty acid oxidation in mouse skeletal muscle via AMPK/ACC signaling.
- Bioinformatics analysis links the IL6/ERK1/2 pathway to fatty acid metabolism-related genes in both mice and humans, indicating its broad metabolic control.
- The study shows that the α2-adrenergic pathway is necessary for IL6's effect on muscle metabolism, as blocking the IL6 receptor in the VMH disrupts exercise-induced fatty acid oxidation.
Neutralisation of SARS-CoV-2 lineage P.1 by antibodies elicited through natural SARS-CoV-2 infection or vaccination with an inactivated SARS-CoV-2 vaccine: an immunological study.
Lancet Microbe
October 2021
Background: Mutations accrued by SARS-CoV-2 lineage P.1-first detected in Brazil in early January, 2021-include amino acid changes in the receptor-binding domain of the viral spike protein that also are reported in other variants of concern, including B.1.
View Article and Find Full Text PDFMicrobiota-derived short-chain fatty acids do not interfere with SARS-CoV-2 infection of human colonic samples.
Gut Microbes
February 2021
Microbiota-derived molecules called short-chain fatty acids (SCFAs) play a key role in the maintenance of the intestinal barrier and regulation of immune response during infectious conditions. Recent reports indicate that SARS-CoV-2 infection changes microbiota and SCFAs production. However, the relevance of this effect is unknown.
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