Publications by authors named "Lucas Bochnia-Bueno"
Background: The immunological response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and immunisation is variable.
Objectives: To describe the humoral immune response by correlating IgA and IgG antibodies with NAbs titration following CoronaVac® immunisation and an mRNA (Comirnaty®) booster among healthcare workers (HCWs) and to compare the cytokine and interleukin profiles between HCWs vaccinated with CoronaVac and coronavirus disease 2019 (COVID-19) infected patients.
Methods: Samples from 133 HCWs collected at 20 (T1) and 90 (T2) days after CoronaVac immunisation and 15 (T3) days after a booster dose with the Comirnaty vaccine were analysed for IgA and IgG EIA and neutralisation assay.
Background: This study is aimed at calculating the IgA antibody dynamic range in healthcare workers (HCWs) after immunization with CoronaVac® and Comirnaty® booster dose.
Methods: A total of 118 HCW serum samples from Southern Brazil were collected the day before the first vaccine dose (day 0) and + 20, + 40, + 110, + 200 days following the vaccine's first dose, and + 15 days after a Comirnaty® booster dose. Immunoglobulin A (IgA) was quantified using immunoassays for anti-S1 (spike) protein antibodies (Euroimmun, Lübeck, Germany).
Antibody response following a Comirnaty booster dose in CoronaVac vaccinated individuals.
Diagn Microbiol Infect Dis
August 2022
Serum samples from 106 health care workers were analyzed by enzyme-linked immunosorbent assay (ELISA) test 15 days after a booster dose. A total of 99% of the participants showed a significant increase in the anti-S1 IgG index. The significant humoral response was observed 15 days after the heterologous dose of Comirnaty in most the subjects, regardless of having comorbidities.
View Article and Find Full Text PDFThe nasopharyngeal swab is a gold standard for detecting SARS-CoV-2. However, the inconvenience of this method compelled us to compare its efficiency with saliva and gargle samples, which we collected sequentially from 229 individuals. Saliva outperformed gargle samples, constituting a reliable RNA viral source with similar performance to nasopharyngeal samples.
View Article and Find Full Text PDFSevere acute respiratory syndrome coronavirus 2 infection among healthcare workers in a tertiary public hospital in Curitiba, Brazil.
Rev Soc Bras Med Trop
March 2022
Article Synopsis
- A study conducted in Curitiba, Brazil, aimed to analyze the characteristics of COVID-19 among healthcare workers (HCWs) by testing over 1,000 samples for the virus between June and December 2020.* -
- Results indicated that 32.7% of HCWs tested positive for SARS-CoV-2, with higher infection rates found among symptomatic (39.2%) compared to asymptomatic (15.9%) workers; high-risk hospital departments had the most cases.* -
- The study highlights the importance of early diagnosis and isolation of infected HCWs to help control the spread of the virus, especially since those in direct contact with COVID-19 patients have a higher risk of infection.*
Article Synopsis
- - The study investigated the effectiveness of the CoronaVac vaccine by measuring the seroconversion rate and IgG antibody levels in 133 healthcare workers in Southern Brazil after receiving their first vaccine dose.
- - Blood samples were collected at various intervals (Day 0 and Days +10, +20, +40, +60, +110) to assess levels of antibodies against the spike (S1) and nucleocapsid (N) proteins.
- - Results showed a high seroconversion rate, with 97% of participants developing antibodies for the S1 protein by Day 40, while 51.87% responded for the N protein, highlighting the vaccine's effectiveness in this population.
Objective: To assess the diagnostic performance of lateral flow immunochromatographic assays (LFAs) of 4 different manufacturers to identify SARS-CoV-2 antibodies (IgM, IgG, or total), comparing them with the nucleic acid amplification test (NAAT) or the clinical defined test (definite or probable SARS-CoV-2 infection, respectively).
Methods: One hundred nineteen serum samples were randomly selected by convenience and distributed in the following groups: (1) group with SARS-CoV-2 infection (n = 82; RT-qPCR positive [definite, n = 70] and probable [n = 12]); (2) other diseases (n = 27; other viruses identified [n = 8] and SARS of other etiologies [n = 19]); and (3) healthy control group (n = 10). LFAs of 4 manufacturers were compared: MedTest Coronavirus (COVID-19) IgG/IgM (MedLevensohn, Brazil); COVID-19 IgG/IgM ECO Test (Ecodiagnóstica, Brazil); Camtech COVID-19 IgM/IgG Rapid Test Kit (Camtech Diagnostics Pte Ltd, Singapore); and 1-Step COVID-19 Test for total antibodies (Guangzhou Wondfo Biotech Co.