Nitrogen sources and CO concentration synergistically affect the growth and metabolism of tobacco plants.

The initial stimulation of photosynthesis under elevated CO concentrations (eCO) is often followed by a decline in photosynthesis, known as CO acclimation. Changes in N levels under eCO can have different effects in plants fertilized with nitrate (NO) or ammonium (NH) as the N source. NO assimilation consumes approximately 25% of the energy produced by an expanded leaf, whereas NH requires less energy to be incorporated into organic compounds.

The cryoprotectant PVS2 plays a crucial role in germinating Passiflora ligularis embryos after cryopreservation by influencing the mobilization of lipids and the antioxidant metabolism.

Cryopreservation is a process whereby biological structures are preserved in liquid nitrogen (-196 °C) without losing their viability. Many cryopreservation techniques use the Plant Vitrification Solution 2 (PVS2) for cryoprotection. This study will therefore evaluate the influence of different exposure times to the cryoprotectant PVS2 and discuss the importance of the mobilization of reserves and the antioxidant metabolism during the germination of cryopreserved Passiflora ligularis embryos.