A Multipurpose Metallophore and Its Copper Complexes with Diverse Catalytic Antioxidant Properties to Deal with Metal and Oxidative Stress Disorders: A Combined Experimental, Theoretical, and Study.

We report the discovery that the molecule 1-(pyridin-2-ylmethylamino)propan-2-ol (HL) can reduce oxidative stress in neuronal C6 glioma cells exposed to reactive oxygen species (O, HO, and OH) and metal (Cu) stress conditions. Furthermore, its association with Cu generates [Cu(HL)Cl] () and [Cu(HL)](ClO) () complexes that also exhibit antioxidant properties. Potentiometric titration data show that HL can coordinate to Cu in 1:1 and 1:2 Cu:ligand ratios, which was confirmed by monocrystal X-ray studies.

ROS scavenging of SOD/CAT mimics probed by EPR and reduction of lipid peroxidation in S. cerevisiae and mouse liver, under severe hydroxyl radical stress condition.

The interaction between Cu, Fe and Mn complexes, derived from the ligands 1-[bis(pyridine-2-ylmethyl)amino]-3-chloropropan-2-ol (hpclnol) and bis(pyridine-2-ylmethyl)amine (bpma), and the free radical 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl (DPPH) and reactive oxygen species (ROS), was investigated by colorimetric and EPR (Electron Paramagnetic Resonance) techniques. A comparison between these results and those reported to [Mn(salen)Cl] or EUK-8 was also addressed. EPR studies allowed us the identification of intermediates species such as superoxide‑copper(I) and superoxide‑copper(II), a mixed-valence FeFe species and a 16-line feature attributed to Mn-oxo-Mn species.

Scavenging of reactive species probed by EPR and ex-vivo nanomolar reduction of lipid peroxidation of manganese complexes.

Antioxidant activity toward HO, anion radical superoxide, hydroxyl and DPPH (2,2-diphenyl-1-picrylhydrazyl) of two manganese complexes [Mn(III)(bpa)]Cl.HO (1) and [(Cl)Mn(μ-hbpclnol)(μ-bpclnol)Mn](ClO).3HO (2) (hbpa = (2-hydroxybenzyl-2-pyridylmethyl)amine and hbpclnol = (N-(2-hydroxybenzyl)-N-(2-pyridylmethyl)[(3-chloro)(2-hydroxy)]propylamine) are presented.

Evaluation of DNA-binding and DNA-photocleavage ability of tetra-cationic porphyrins containing peripheral [Ru(bpy)Cl] complexes: Insights for photodynamic therapy agents.

The present work reports the spectroscopic and theoretical evaluation of the interaction between calf-thymus DNA (CT-DNA) and free-base meso-tetra-(ruthenated) porphyrin (HRuTPyP) or its corresponding Zn(II) complex (ZnRuTPyP). Spectroscopic measurements (UV-vis, circular dichroism and steady-state fluorescence emission) combined with theoretical molecular docking calculations suggest that Ru(II)-porphyrins interact with the DNA backbone by external mode via electrostatic forces. In addition, gel electrophoresis analysis demonstrate that these porphyrins promote efficient plasmidial DNA photocleavage upon white-light irradiation conditions, indicating HRuTPyP and ZnRuTPyP as potential candidates for photodynamic therapy.

Preliminary evaluation of the positively and negatively charge effects of tetra-substituted porphyrins on photoinactivation of rapidly growing mycobacteria.

This manuscript reports, at the first time, the photoinactivation evaluation of tetra-cationic and anionic porphyrins as photosensitizers (PS) for the photodynamic inactivation (PDI) of rapidly growing mycobacteria strains. Two different charged porphyrin groups were obtained commercially. PDI experiments in the strains Mycobacterium massiliense e Mycobacterium fortuitum conducted with adequate concentration (without aggregation) of photosensitizer under white light at a fluence rate of 50 mW/cm over 90 min showed that the most effective PS caused a 100 times reduction in the concentration of viable mycobacteria.