Publications by authors named "Luc Jw van der Laan"
Cholangiocarcinoma (CCA) is a type of liver cancer with an aggressive phenotype and dismal outcome in patients. The metastasis of CCA cancer cells to distant organs, commonly lung and lymph nodes, drastically reduces overall survival. However, mechanistic insight how CCA invades these metastatic sites is still lacking.
View Article and Find Full Text PDFPatient-derived tumor organoids have been established as promising tools for in vitro modelling of multiple tumors, including cholangiocarcinoma (CCA). However, organoids are commonly cultured in basement membrane extract (BME) which does not recapitulate the intricacies of the extracellular matrix (ECM). We combined CCA organoids (CCAOs) with native tumor and liver scaffolds, obtained by decellularization, to effectuate a model to study the interaction between epithelial tumor cells and their surrounding ECM.
View Article and Find Full Text PDFThe molecular events that drive hepatitis B virus (HBV)-mediated transformation and tumorigenesis have remained largely unclear, due to the absence of a relevant primary model system. Here we propose the use of human liver organoids as a platform for modeling HBV infection and related tumorigenesis. We first describe a primary ex vivo HBV-infection model derived from healthy donor liver organoids after challenge with recombinant virus or HBV-infected patient serum.
View Article and Find Full Text PDFAim: To investigate the specific effects of immunosuppressants on the antiviral action of daclatasvir and asunaprevir.
Methods: The antiviral activity of daclatasvir (DCV) and asunaprevir (ASV) combined with immunosuppressants was tested using two models for hepatitis C virus (HCV) infection.
Results: Tacrolimus, rapamycin and cyclosporine did not negatively affect the antiviral action of DCV or ASV.
Article Synopsis
- Scientists are working on new ways to grow liver cancer cells in the lab to understand them better.
- They created special mini-livers called organoids that behave like real liver tissue, keeping important features of the original cancer.
- These organoids can help researchers find new treatments and better understand how liver cancer works.
Aim: To investigate the effects of mammalian target of rapamycin (mTOR) inhibition on liver regeneration and autophagy in a surgical resection model.
Methods: C57BL/6 mice were subjected to a 70% partial hepatectomy (PH) and treated intraperitoneally every 24 h with a combination of the mTOR inhibitor rapamycin (2.5 mg/kg per day) and the steroid dexamethasone (2.