A small-molecule compound belonging to a class of 2,4-disubstituted 1,3,4-thiadiazine-5-ones inhibits intracellular growth and persistence of Chlamydia trachomatis.

Chlamydia trachomatis is one of the most common sexually transmitted pathogens in the world and often causes chronic inflammatory diseases that are insensitive to antibiotics. The type 3 secretion system (T3SS) of pathogenic bacteria is a promising target for therapeutic intervention aimed at bacterial virulence and can be an attractive alternative for the treatment of chronic infections. Recently, we have shown that a small-molecule compound belonging to a class of 2,4-disubstituted 1,3,4-thiadiazine-5-ones produced through the chemical modification of the thiohydrazides of oxamic acids, designated CL-55, inhibited the intracellular growth of C.

The characteristics of ubiquitous and unique Leptospira strains from the collection of Russian centre for leptospirosis.

Background And Aim: Leptospira, the causal agent of leptospirosis, has been isolated from the environment, patients, and wide spectrum of animals in Russia. However, the genetic diversity of Leptospira in natural and anthropurgic foci was not clearly defined.

Methods: The recent MLST scheme was used for the analysis of seven pathogenic species.

Detection of C. trachomatis in the serum of the patients with urogenital chlamydiosis.

Extragenital chlamydial complications may be associated with systemic spread of infection, but haematogenous route for C. trachomatis dissemination has not been clearly demonstrated. Here we report that serum specimens obtained from patients with chlamydiosis contain elementary bodies of C.

Isolation of Chlamydia pneumoniae from serum samples of the patients with acute coronary syndrome.

Background: Limited body of evidence suggests that lipopolysaccharide of C. pneumoniae as well as C. pneumoniae-specific immune complexes can be detected and isolated from human serum.

First detection of a viral agent causing disease in farmed sturgeon in Russia.

A virus was isolated from Siberian sturgeon Acipenser baeri and bester (beluga Huso huso x sterlet A. ruthenus hybrid) fingerlings in SSO-2, SSF-2 and WSSK-1 cell lines during an acute epizootic on a large fish farm producing fertilised sturgeon eggs and fry. Transmission electron microscopic examination of samples from both inoculated cell cultures and skin of affected fish revealed viral particles with a herpesvirus-like morphology.

Application of Transmission Electron Microscopy for Analysis of Ultrastructural Organization of Circulating Immune Complexes in Patients with Typhoid Fever and Chronic Carriers of Salmonella typhi.

Transmission electron microscopy was used to study the ultrastructural organization of circulating immune complexes (CIC), isolated from patients with typhoid fever in the different periods of acute infectious process (febrile period, period of an early and late reconvalescence), relapse and from acute and chronic carriers of Salmonella typhi in the period of pathogen excreting. It has been shown that preparations of CIC from healthy donors consisted of amorphous mean electron density material, including a cell-like detritus. At acute and chronic infectious process there were bacterial cells in a structure of the CIC.