Effect of neonatal β(3)-adrenoceptor agonist CL 316,243 treatment on body fat accumulation and intestinal alkaline phosphatase activity in rats from reduced nests.

Introduction: The aim of this study was to evaluate the effects of early life administration of β3-adrenoceptor agonist (CL 316,243) on somatic and feeding parameters, obesity development as well as small intestinal enzyme activity in 21- and 40-day-old overfed male Sprague-Dawley rats.

Material And Methods: To induce postnatal overnutrition, litter size was reduced to 4 pups/litter (small litters, SL); while in normally-nourished groups (NL) the litter size was adjusted to 10 pups/litter. From days 5 to 15, half of the suckling pups from NL and SL groups received CL 316,243 subcutaneously.

Effect of repeated fasting/refeeding on obesity development and health complications in rats arising from reduced nest.

Background: Overnutrition during postnatal life represents a risk factor for later obesity and associated metabolic disorders.

Aim: We investigated the interaction between postnatal and later-life nutrition on body composition, blood pressure and the jejunal enzyme activities in male Sprague-Dawley rats.

Methods: From birth, we adjusted the number of pups in the nest to 4 (small litters-SL; overfeeding) or to 10 pups (normal litters-NL; controls), and from day 50 until 70, the SL (SL-R) and NL (NL-R) rats were subjected to 1 day fasting and 1 day refeeding cycles (RFR).

Long-term effect of altered nutrition induced by litter size manipulation and cross-fostering in suckling male rats on development of obesity risk and health complications.

Objective: We investigated the long-term effect of pre-weaning nutrition on positive and/or adverse regulation of obesity risk and health complications in male Sprague-Dawley rats.

Design And Subject: Two experimental models were used in the present work: (1) To induce postnatal over- or normal nutrition, the litter size was adjusted to 4 (small litters-SL) and to 10 pups (normal litters-NL) in the nest, (2) in suckling pups at day 10, we used cross-fostering to identify the effect of altered dietary environment on their future body fat regulation, food intake, blood pressure, and the duodenal and jejunal alkaline phosphatase activity. After weaning, these control (NL, SL) and cross-fostered (NL-SL, SL-NL) groups were exposed to standard laboratory diet.

Effect of antibiotic treatment on intestinal microbial and enzymatic development in postnatally overfed obese rats.

Objective: To investigate the effect of the microbiota-induced changes and early overfeeding after amoxicillin administration (a) in suckling pups via their dams up to 15 days of lactation and (b) in weaned pups on intestinal microbial/functional adaptability and obesity development in male Sprague-Dawley rats.

Design And Methods: Postnatal nutrition was elicited by adjusting the number of pups in the nest to 4 (small litters [SLs]) and 10 (normal litters [NLs]), while from days 21 to 40, both groups were fed with a standard diet. The numbers of Bacteroides/Prevotella (BAC) and Lactobacillus/Enterococcus (LAB) in the jejunum and colon were determined by fluorescence in situ hybridization technique, and jejunal alkaline phosphatase (AP), α-glucosidase and aminopeptidase activity was assayed histochemically.