Phylogenetic diversity and morphological characterization of cordycipitaceous species in Taiwan.

Species classified in () include multiple entomopathogenic fungi. Numerous changes have recently occurred in the nomenclature of cordycipitaceous fungi due to the single naming system proposed for pleomorphic fungi in 2011. Species of are widely applied as herbal medicines, especially in Asian cultures.

Integrative taxonomy of Metarhizium anisopliae species complex, based on phylogenomics combined with morphometrics, metabolomics, and virulence data.

Metarhizium anisopliae (Clavicipitaceae, Hypocreales) is a globally distributed entomopathogenic fungus, which has been largely studied and used in agriculture for its potent entomopathogenicity. Since its taxonomic establishment as a member of Metarhizium, many closely related taxa have been described with highly similar morphology (cryptic species). A holotype specimen of M.

Fungal Planet description sheets: 1614-1696.

Novel species of fungi described in this study include those from various countries as follows: , in leaves of , among deep leaf litter, from uredinium of on , on well-rotted twigs and litter in mixed wet sclerophyll and subtropical rainforest. , on twigs of , on bark, in savannas with shrubs and trees. , on leaves of , (incl.

New and Interesting Fungi. 7.

Two new genera, 17 new species, two epitypes, and six interesting new host and / or geographical records are introduced in this study. New genera include: (based on ) and (based on ). New species include: (from hypersaline sea water, Qatar), (from mycangia of , USA), (on leaves of , Brazil), (from pre-stored , South Africa), (from soil, South Africa), (from dead capsule of , Germany), (from house dust, Canada), (from soil, South Africa), (on ascomata of ascomycete on twigs of , South Africa), (on leaf litter, Spain), (on larva, Thailand), (from soil, South Africa), (from pasture mulch, South Africa), (from soil, South Africa), (on stem, bark of living tree of sp.

Bioactive Bioxanthracene and Cyclodepsipeptides from the Entomopathogenic Fungus BCC56290.

In the course of our ongoing research targeting the identification of potential biocontrol agents from entomopathogenic fungi (EPF), we explored a solid-state rice fungal extract of BCC56290 derived from infected lepidopteran larvae. Chemical and biological prospections afforded four unprecedentedly reported natural products differentiated into a dimeric naphthopyran bioxanthracene ES-242 derivative () and three cyclodepsipeptides (-) in addition to two known cyclodepsipeptides, cardinalisamides B () and C (). Chemical structures of the isolated compounds were elucidated through comprehensive 1D/2D NMR and HR-ESI-MS data together with comparisons to the reported literature.

Fungal Planet description sheets: 1478-1549.

Novel species of fungi described in this study include those from various countries as follows: , on whitefly, on bark of , from soil under , on leaf spot of , and on leaf spot of . , on fully submersed siliceous schist in high-mountain streams, and on the lower part and apothecial discs of on a twig. , on decaying wood, from moist soil with leaf litter, on a trunk of a living unknown hardwood tree species, and on dead twigs of unidentified plant.

New mycoparasitic species in the genera and . (: ), with sporothrix-like asexual morphs, from Thailand.

Four new species of the genus are described from Thailand. They were found as mycoparasites on: infecting flies () for ; ants () for ; and leafhoppers () for and . A new genus, with two species: (type species), parasitic on infecting cockroaches, and , found on a spider egg sac, are also described.

Bioprospection of Tenellins Produced by the Entomopathogenic Fungus .

Fungi are known as prolific producers of bioactive secondary metabolites with applications across various fields, including infectious diseases, as well as in biological control. However, some of the well-known species are still underexplored. Our current study evaluated the production of secondary metabolites by the entomopathogenic fungus from Thailand.

Targeted sequencing analysis pipeline for species identification of human pathogenic fungi using long-read nanopore sequencing.

Among molecular-based techniques for fungal identification, Sanger sequencing of the primary universal fungal DNA barcode, the internal transcribed spacer (ITS) region (ITS1, 5.8S, ITS2), is commonly used in clinical routine laboratories due to its simplicity, universality, efficacy, and affordability for fungal species identification. However, Sanger sequencing fails to identify mixed ITS sequences in the case of mixed infections.

Segregation of the genus (Hypoxylaceae, Xylariales) from by a polyphasic taxonomic approach.

During a mycological survey of the Democratic Republic of the Congo, a fungal specimen that morphologically resembled the American species was encountered. A polyphasic approach including morphological and chemotaxonomic together with a multigene phylogenetic study (ITS, LSU, , and ) of spp. and representatives of related genera revealed that this strain represents a new species of the Hypoxylaceae.

Studies on the Genus (Hypoxylaceae) in Thailand Using a Polyphasic Taxonomic Approach.

Over the past two decades, hypoxylaceous specimens were collected from several sites in Thailand. In this study, we examined their affinity to the genus using macroscopic and microscopic morphological characters, dereplication of their stromatal secondary metabolites using ultrahigh performance liquid chromatography coupled to diode array detection and ion mobility tandem mass spectrometry (UHPLC-DAD-IM-MS/MS), and molecular phylogenetic analyses. We describe and illustrate five novel species and a new record for the country, present multi-locus phylogenetic analyses that show the distinction between the proposed species, and provide proteomic profiles of the fungi using matrix associated laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF/MS) for the first time.

Mushroom poisoning in Thailand between 2003 and 2017.

The frequent occurrence of mushroom poisoning cases in the wet season in Thailand has long been recognized but has never been quantitatively analyzed. This study aims to analyze mushroom poisoning cases in Thailand between 2003 and 2017 and focused on their association with the rainfall. The results revealed 22,571 cases and 106 deaths in this period.

Fungal Planet description sheets: 1383-1435.

Novel species of fungi described in this study include those from various countries as follows: , , and on soil, from stem cankers of , from stem of , and from leaves of , as endophyte from roots of , from stem of , from leaves of × and from roots of , from intertidal wood and (incl. gen. nov.

Is Congeneric with ? Phylogenetic Relationships of spp. (, ) and a New Genus on Scale Insects in Thailand.

During surveys of insect pathogenic fungi (IPF) in Thailand, fungi associated with scale insects and plants were found to represent five new species of the genus in . Their macroscopic features resembled both and . Morphological comparisons with the type and known and species and phylogenetic evidence from a multigene dataset support the appointment of a new species of .

New species in , and a new genus associated with plants in from Thailand.

Three new fungal species in the (, ) associated with plants were collected in Thailand. Morphological characterisation and phylogenetic analyses based on multi-locus sequences of LSU, and showed that two species belong to and occupies a unique clade and is proposed as a novel genus in and share the morphological characteristic of having cylindrical to clavate stromata arising from seeds. produces perithecial plates and occurs on a leaf sheath of an unknown panicoid grass.

Fungal Planet description sheets: 1284-1382.

Novel species of fungi described in this study include those from various countries as follows: , from coastal sea sand. , on soil, on dead wood, from roots and leaves of and from capsules of , (incl. gen.

The integrative taxonomy of and species complexes with whole-genome sequencing, morphometric and chemical analyses.

Fungi are rich in complexes of cryptic species that need a combination of different approaches to be delimited, including genomic information. (, ) is a well-known genus of entomopathogenic fungi, used as a biocontrol agent. In this study we present a polyphasic taxonomy regarding two widely distributed complexes of : and s.

sp. nov. and a Record of in Thailand.

During the rainy season in Thailand, specimens of sp. nov. and were collected from Chiang Mai and Samut Sakhon Provinces, respectively.

Using High-Throughput Amplicon Sequencing to Evaluate Intragenomic Variation and Accuracy in Species Identification of Species.

While recent sequencing technologies (third generation sequencing) can successfully sequence all copies of nuclear ribosomal DNA (rDNA) markers present within a genome and offer insights into the intragenomic variation of these markers, high intragenomic variation can be a source of confusion for high-throughput species identification using such technologies. High-throughput (HT) amplicon sequencing via PacBio SEQUEL I was used to evaluate the intragenomic variation of the ITS region and D1-D2 LSU domains in nine species, and the accuracy of such technology to identify these species based on molecular phylogenies was also assessed. PacBio sequences within strains showed variable level of intragenomic variation among the studied species with showing greater variation than the others.

Fungal Planet description sheets: 1112-1181.

Novel species of fungi described in this study include those from various countries as follows: , on soil, on leaves of on leaves of on leaves of sp., on soil, (incl. gen.

Fungal Planet description sheets: 1182-1283.

Novel species of fungi described in this study include those from various countries as follows: , from , from soil. , as endophyte from healthy leaves of , in fruit of , from stem of , on stems of , from rhizosphere soil of , on living leaves of , , and on living leaves of sp. , from soil.

Fungal pathogens occurring on in Thailand.

Two new fungal genera and six species occurring on insects in the orders Orthoptera and Phasmatodea (superorder Orthopterida) were discovered that are distributed across three families in the . Sixty-seven sequences generated in this study were used in a multi-locus phylogenetic study comprising SSU, LSU, , and together with the nuclear intergenic region (IGR). These new taxa are introduced as , , , , and .