Publications by authors named "Luana S Delcaro"
Objective: Syncytia formation is the hallmark of the cytopathic effect caused by human respiratory syncytial virus (HRSV), which is the most important viral respiratory pathogen in children. This article reports methodological improvements in primary HRSV isolation and the importance of syncytia formation and mRNA levels of F protein for the progeny yield, using clinical isolates of HRSV.
Methods: The A and B strains of HRSV were isolated in HEp-2 cell cultures from fresh and frozen nasopharyngeal aspirates.
Article Synopsis
- One of the patients represents the first documented OROV case outside the Amazon.
- Researchers discovered OROV in peripheral blood leukocytes, a finding that could improve understanding of the virus's impact and aid in rapid diagnosis of this overlooked infection.
Background: Chronic rhinosinusitis (CRS) is a common illness, yet little is known about its pathogenesis, including the role played by respiratory viruses.
Methods: A transversal prospective study was conducted to analyze the seasonality of CRS using real-time polymerase chain reaction to detect respiratory virus genomes in secretions and tissue samples from patients with CRS with and without nasal polyps.
Results: The frequency of viral detection was 41% (31/75).
Respiratory viruses are continuously detected in children with chronic tonsillitis throughout the year.
Int J Pediatr Otorhinolaryngol
October 2014
Objective: To evaluate the oscillations on the viral detection in adenotonsillar tissues from patients with chronic adenotonsillar diseases as an indicia of the presence of persistent viral infections or acute subclinical infections.
Study Design: Cross-sectional prospective study.
Setting: Tertiary hospital.
Background: Lower respiratory tract infection (LRTI) is a major cause of pediatric morbidity and mortality, especially among non-affluent communities. In this study we determine the impact of respiratory viruses and how viral co-detections/infections can affect clinical LRTI severity in children in a hospital setting.
Methods: Patients younger than 3 years of age admitted to a tertiary hospital in Brazil during the months of high prevalence of respiratory viruses had samples collected from nasopharyngeal aspiration.