Production of a Promising Biosynthetic Self-Assembled Nanoconjugate Vaccine against Klebsiella Pneumoniae Serotype O2 in a General Escherichia Coli Host.

Klebsiella pneumoniae has emerged as a severe opportunistic pathogen with multiple drug resistances. Finding effective vaccines against this pathogen is urgent. Although O-polysaccharides (OPS) of K.

Magnolol attenuates inflammatory pain by inhibiting sodium currents in mouse dorsal root ganglion neurons.

Voltage-gated sodium channels are currently recognized as one of the targets of analgesics. Magnolol (Mag), an active component isolated from Magnolia officinalis, has been reported to exhibit analgesic effects. The objective of this study was to investigate whether the analgesic effect of Mag was associated with blocking Na channels.

Polycyclic aromatic hydrocarbons and nitro-polycyclic aromatic hydrocarbons in five East Asian cities: Seasonal characteristics, health risks, and yearly variations.

Total suspended particulate matter and fine particulate matter were collected in five East Asian cities (Sapporo, Sagamihara, Kirishima, Shenyang, and Vladivostok) during warm and cold periods from 2017 to 2018. Nine polycyclic aromatic hydrocarbons (PAHs) and three nitro-polycyclic aromatic hydrocarbons (NPAHs) were detected by high-performance liquid chromatography with a fluorescence detector. The average concentrations of ∑PAHs and ∑NPAHs differed significantly both temporally and spatially and were the lowest in Kirishima during the warm period (∑PAHs: 0.

Hydroxytyrosol alleviates oxidative stress and neuroinflammation and enhances hippocampal neurotrophic signaling to improve stress-induced depressive behaviors in mice.

Hydroxytyrosol (HT), the main phenolic compound in olives and olive products, has antioxidative, anti-inflammatory, neuroprotective, and other physiological functions. The effects of HT on depression are unclear. The aim of this study was to explore the effects of HT on chronic unpredictable mild stress (CUMS) induced depressive-like behaviors.

Protective effects of melatonin on the white matter damage of neonatal rats by regulating NLRP3 inflammasome activity.

Objective: The aim of the study was to investigate the protective effects and relevant mechanisms of melatonin on the white matter damage (WMD) caused by endotoxin and ischemic hypoxia in neonatal rats.

Methods: Seventy-two female neonatal rats (postnatal day 3) were randomly divided into the sham, melatonin-treated, and control groups (n = 24 for each group). The periventricular white matter was collected to evaluate the WMD and apoptosis.

Involvement of LIMK2 in actin cytoskeleton remodeling during the definitive endoderm differentiation.

LIM kinases are involved in various cellular events such as migration, cycle, and differentiation, but whether they have a role in the specification of mammalian early endoderm remains unclear. In the present study, we found that depletion of LIMK2 severely inhibited the generation of definitive endoderm (DE) from human embryonic stem cells (hESCs) and promoted an early neuroectodermal fate. Upon the silencing of LIMK2 during the endodermal differentiation, the assembly of actin stress fibers was disturbed, and the phosphorylation of cofilin was decreased.

Effect of short-term intake of four sweeteners on feed intake, solution consumption and neurotransmitters release on mice.

This study focused on the effect of short-term intake of sweeteners on feed intake, solution consumption and neurotransmitters release on mice. The results showed that the free drinking of 10 mM sucralose solution, 100 mM maltose solution, 3 mM saccharin solution and 3 g/L stevioside solution for 32 days will not affect the normal development of the body weight and feed intake of the mice. The consumption of maltose solution was significantly higher than that of the other sweeteners.

Screening a Strain of sp. O852 and the Optimization of Fermentation Conditions for -Dihydrocarvone Production.

Flavors and fragrances have high commercial value in the food, cosmetic, chemical and pharmaceutical industries. It is interesting to investigate the isolation and characterization of new microorganisms with the ability to produce flavor compounds. In this study, a new strain of sp.

Polyvinylpyrrolidone Assisted Preparation of Highly Conductive, Antioxidation, and Durable Nanofiber Composite with an Extremely High Electromagnetic Interference Shielding Effectiveness.

With booming development of electronics, electromagnetic interference (EMI) shielding materials based on conductive polymer composites (CPCs) have received increasing attention. However, it remains challenging to develop flexible and lightweight CPCs with excellent stretchability, breathability, durability, and high EMI shielding effectiveness (EMI SE). Here, we propose a facile polyvinylpyrrolidone (PVP) assisted preparation of highly electrically conductive and durable nanofiber composites for high performance EMI shielding.

Associations of spicy food flavour and intake frequency with blood lipid levels and risk of abnormal serum lipid levels in Chinese rural population: a cross-sectional study.

Objective: The purpose of the current study was to investigate associations between spicy food intake and serum lipids levels in Chinese rural population.

Design: Information on spicy food flavour and intake frequency was obtained using a two-item questionnaire survey. Dietary data were collected using a validated thirteen-item FFQ.

Molecular dynamics simulations data of six compounds F3J-BRD4/CBP, EX1-BRD4/CBP, and E2T-BRD4/CBP.

The data here described are related to the research article entitled "Molecular dynamics insights into binding selectivity of inhibitors toward BRD4 and CBP" [1]. Bromodomain-containing protein 4 (BRD4) and CREB binding protein (CBP) play important roles in tumorigenesis and development. We performed 200-ns molecular dynamics (MD) simulations on three pairs of inhibitor-BRD4 and inhibitor-CBP complexes to clarify binding selectivity of inhibitors toward BRD4 and CBP.

Polyphenols from Induce Apoptosis of HepG2 Cells via Inactivation of ERK and AKT Signaling Pathways.

The extract of has been proved to have antitumor activity. However, the underlying mechanism remains unclear. This study aimed to elucidate the mechanism of apoptosis of HepG2 cells induced by polyphenols from (PBPs).

Endogenous sulfur dioxide is a novel inhibitor of hypoxia-induced mast cell degranulation.

Introduction: Mast cell (MC) degranulation is an important step in the pathogenesis of inflammatory reactions and allergies; however, the mechanism of stabilizing MC membranes to reduce their degranulation is unclear.

Methods: SO content in MC culture supernatant was measured by HPLC-FD. The protein and mRNA expressions of the key enzymes aspartate aminotransferase 1 (AAT1) and AAT2 and intracellular AAT activity were detected.

The Candidate Photoperiod Gene Promotes Growth and Flowering in .

Flowering time influences the yield and productivity of legume crops. is a reference temperate legume that, like the winter annual , shows accelerated flowering in response to vernalization (extended cold) and long-day (LD) photoperiods (VLD). However, unlike , appears to lack functional homologs of core flowering time regulators () and () which act upstream of the mobile florigen FLOWERING LOCUS T (FT).

Nomogram for predicting swallowing recovery in patients after dysphagic stroke.

Background: To date, variables predicting the recovery of dysphagia in patients after dysphagic stroke have not been well defined. However, despite the difficulties in predicting and understanding the dysphagia recovery trajectory, its significance for stroke care cannot be understated. This study aims to identify the factors for functional swallowing recovery and develop nomograms that predict dysphagia recovery after stroke.

Seepage Time Soft Sensor Model of Nonwoven Fabric Based on the Extreme Learning Machine Integrating Monte Carlo.

Nonwoven fiber materials are materials with multifunctional purposes, and are widely used to make masks for preventing the new Coronavirus Disease 2019. Because of the complexity and particularity of their structure, it becomes difficult to model the penetration and flow characteristics of liquid in nonwoven fiber materials. In this paper, a novel seepage time soft sensor model of nonwoven fabric, based on Monte Carlo (MC), integrating extreme learning machine (ELM) (MCELM) is proposed.

Characteristics and Health Risks of Polycyclic Aromatic Hydrocarbons and Nitro-PAHs in Xinxiang, China in 2015 and 2017.

Fine particulate matter (PM) samples were collected in the summer and winter of 2015 and 2017 in Xinxiang, China. Nine polycyclic aromatic hydrocarbons (PAHs) and three nitro-PAHs (NPAHs) in PM were detected via high-performance liquid chromatography (HPLC). The PAHs concentration in summer and winter decreased from 6.

Assessing Approaches of Human Inhalation Exposure to Polycyclic Aromatic Hydrocarbons: A Review.

Polycyclic aromatic hydrocarbons (PAHs) are a class of important organic pollutants widely emitted from anthropogenic activities, with a general distribution in the gas and particulate phases. Some PAHs are carcinogenic, teratogenic, and mutagenic. Inhalation exposure to PAHs is correlated with adverse health outcomes in the respiratory and cardiovascular systems.

Exposure to Atmospheric Particulate Matter-Bound Polycyclic Aromatic Hydrocarbons and Their Health Effects: A Review.

Particulate matter (PM) is a major factor contributing to air quality deterioration that enters the atmosphere as a consequence of various natural and anthropogenic activities. In PM, polycyclic aromatic hydrocarbons (PAHs) represent a class of organic chemicals with at least two aromatic rings that are mainly directly emitted via the incomplete combustion of various organic materials. Numerous toxicological and epidemiological studies have proven adverse links between exposure to particulate matter-bound (PM-bound) PAHs and human health due to their carcinogenicity and mutagenicity.

A Novel WRKY Transcription Factor HmoWRKY40 Associated with Betalain Biosynthesis in Pitaya () through Regulating .

Betalains are water-soluble nitrogen-containing pigments with multiple bioactivities. Pitaya is the only large-scale commercially grown fruit containing abundant betalains for consumers. However, the upstream regulators in betalain biosynthesis are still not clear.

Lycorine inhibits cell proliferation, migration and invasion, and primarily exerts cytostatic effects in human colorectal cancer via activating the ROS/p38 and AKT signaling pathways.

Colorectal cancer (CRC) is a life‑threatening malignant tumor of the digestive tract. Diverse gene mutations and complicated alterations to the signaling pathways in CRC lead to heterogeneity in response to chemotherapy. Moreover, anticancer drugs for CRC chemotherapy are limited due to adverse events.

Endogenous SO-dependent Smad3 redox modification controls vascular remodeling.

Sulfur dioxide (SO) has emerged as a physiological relevant signaling molecule that plays a prominent role in regulating vascular functions. However, molecular mechanisms whereby SO influences its upper-stream targets have been elusive. Here we show that SO may mediate conversion of hydrogen peroxide (HO) to a more potent oxidant, peroxymonosulfite, providing a pathway for activation of HO to convert the thiol group of protein cysteine residues to a sulfenic acid group, aka cysteine sulfenylation.

Design and Immunological Evaluation of a Hybrid Peptide as a Potent TLR2 Agonist by Structure-Based Virtual Screening.

Immunity is a versatile defensive response that is involved in protecting against disease by identifying and destroying self and non-self harmful substances. As a state of temporary or permanent immune dysfunction, immunosuppression can make an organism more susceptible to infection, organ injury, and cancer due to damage to the immune system. It has taken a long time to develop new immunomodulatory agents to prevent and treat immunosuppressive diseases.

Impact of COVID-19 Outbreak on the Long-Range Transport of Common Air Pollutants in KUWAMS.

As a background sampling site in western Japan, the Kanazawa University Wajima Air Monitoring Station (KUWAMS) continuously observes the air pollutants, including PM, PM, organic carbon (OC) and element carbon (EC). Data for September 2019 to April 2020 were compared with data for September 2018 to April 2019. The mean concentrations of both PM and PM were 4.