Tuning the spin state of the iron center by FePc/Mg(OH) heterojunction boosting oxygen reduction performance.

Iron phthalocyanine (FePc) is a promising non-noble metal catalyst for oxygen reduction reaction (ORR). While, with the plane-symmetric FeN site, the ORR activity of FePc is generally low due to its low ability to adsorb and activate O. Herein, we anchor FePc on Mg(OH)/N-doped carbon nanosheets building the ternary plate-like catalyst FePc/Mg(OH)/NC.

Tuning Nitrogen Configurations in Nitrogen-Doped Graphene Encapsulating FeC Nanoparticles for Enhanced Nitrate Electroreduction.

Electrochemical nitrate reduction reaction (NORR) offers a promising technology for the synthesis of ammonia (NH) and removal of nitrate in wastewater. Herin, we fabricate a series of FeC nanoparticles in controllable pyridinic-N doped graphene (FeC@NG-X) by a self-sacrificing template method for the NORR. FeC@NG-10 exhibits high catalytic performance with a Faradaic efficiency (FE) of 94.

Genetic characteristics of clinical carbapenem-resistant Klebsiella pneumoniae: epidemic ST11 KPC-2-producing strains and non-negligible NDM-5-producing strains with diverse STs.

Klebsiella pneumoniae is among the most important Gram-negative pathogens that can cause serious nosocomial infections. The emergence and prevalence of hypervirulent carbapenem-resistant K. pneumoniae (Hv-CRKP) pose a significant challenge to public health.

RfaH contributes to maximal colonization and full virulence of hypervirulent .

Hypervirulent (hvKp) have emerged as clinically important pathogens, posing a serious threat to human health. RfaH, a transcriptional elongation factor, has been regarded as implicated in facilitating the transcription of long virulence operons in certain bacterial species. In , RfaH plays a vital role in promoting CPS synthesis and hypermucoviscosity, as well as mediating bacterial fitness during lung infection.

Deep Electron Redistributions Induced by Dual Junctions Facilitating Electroreduction of Dilute Nitrate to Ammonia.

The electronic states of metal catalysts can be redistributed by the rectifying contact between metal and semiconductor e.g., N-doped carbon (NC), while the interfacial regulation degree is very limited.

Transcriptome analysis of long non-coding RNA and mRNA Profiles in VSV-infected BHK-21 Cells.

Background: Vesicular stomatitis virus (VSV) is a typical non-segmented negative-sense RNA virus of the genus Vesiculovirus in the family Rhabdoviridae. VSV can infect a wide range of animals, including humans, with oral blister epithelial lesions. VSV is an excellent model virus with a wide range of applications as a molecular tool, a vaccine vector, and an oncolytic vector.

Isolation and characterization of three novel lytic phages against K54 serotype carbapenem-resistant hypervirulent .

The emergence of carbapenem-resistant hypervirulent (CR-hvKP) has driven us to explore alternative treatments for the limitation of antimicrobial agents. Lytic phages are considered a promising alternative treatment for CR-hvKP infection. In this study, we reported three novel lytic phages, vB_KpnA_SCNJ1-Z, vB_KpnS_SCNJ1-C, and vB_KpnM_SCNJ1-Y, against a CR-hvKP strain SCNJ1, and they possess genomes of double-stranded DNA with a size of 43,428 bp, 46,039 bp, and 50,360 bp, respectively.

Genomic characterization of tigecycline-resistant and isolates from hospital sewage.

Introduction: The tigecycline-resistant Enterobacterales have emerged as a great public concern, and the mobile (X) variants and efflux pump are mainly responsible for the spread of tigecycline resistance. Hospital sewage is considered as an important reservoir of antimicrobial resistance, while tigecycline resistance in this niche is under-researched.

Methods: In this study, five and six strains were selected from a collection of tigecycline-resistant Enterobacterales for further investigation by antimicrobial susceptibility testing, conjugation, whole-genome sequencing, and bioinformatics analysis.

Deep Electronic State Regulation through Unidirectional Cascade Electron Transfer Induced by Dual Junction Boosting Electrocatalysis Performance.

Unidirectional cascade electron transfer induced by multi-junctions is essential for deep electronic state regulation of the catalytic active sites, while this advanced concept has rarely been investigated in the field of electrocatalysis. In the present work, a dual junction heterostructure (FePc/L-R/CN) is designed by anchoring iron phthalocyanine (FePc)/MXene (L-Ti C -R, R═OH or F) heterojunction on g-C N nanosheet substrates for electrocatalysis. The unidirectional cascade electron transfer (g-C N → L-Ti C -R → FePc) induced by the dual junction of FePc/L-Ti C -R and L-Ti C -R/g-C N makes the Fe center electron-rich and therefore facilitates the adsorption of O in the oxygen reduction reaction (ORR).

Genetic analysis of resistance and virulence characteristics of clinical multidrug-resistant isolates.

Objective: is the one of most important pathogens of catheter-associated urinary tract infections. The emergence of multidrug-resistant (MDR) severely limits antibiotic treatments, which poses a public health risk. This study aims to investigate the resistance characteristics and virulence potential for a collection of clinical isolates.

Enhanced electrochemical CO reduction performance of cobalt phthalocyanine with precise regulation of electronic states.

Herein, we report a facile strategy for constructing hybrid coordination configurations by combining functionalized graphene quantum dots (GQDs) with CoPc (CoPc/R-GQDs, with R being -NH or -OH) for electrochemical CO reduction. Benefiting from the high density of functional groups that can be provided by GQDs and the strong electron-donating property of -NH, the examined CoPc/NH-GQDs achieved a 100% faradaic efficiency for CO formation (FE) at -0.8 to -0.

Tuning the Hydroxyl Density of MXene to Regulate the Electrochemical Performance of Anchored Cobalt Phthalocyanine for CO Reduction.

Precise electronic state regulation through coordination environment optimization by metal-support interaction is a promising strategy to facilitate catalysis reaction, while the limited density of functional groups in the bulk substrate restricts the regulation degree. Herein, different sizes of TiCT MXene with hydroxyl (-OH) terminal including the MXene layer (ML-OH, 3 μm), the MXene nanosheet (MNS-OH, 600 nm), and the MXene quantum dot (MQD-OH, 8 nm) were prepared to anchor CoPc, and the effect of -OH density on the performance of electrochemical CO reduction was systematically investigated. Notably, a linear relationship was established by plotting reactivity vs hydroxyl density.

Dopant-Induced Electronic States Regulation Boosting Electroreduction of Dilute Nitrate to Ammonium.

Electrochemically converting NO into NH offers a promising route for water treatment. Nevertheless, electroreduction of dilute NO is still suffering from low activity and/or selectivity. Herein, B as a modifier was introduced to tune electronic states of Cu and further regulate the performance of electrochemical NO reduction reaction (NO RR) with dilute NO concentration (≤100 ppm NO -N).

A new class A beta-lactamase gene bla coexists with bla in a novel untypable plasmid in Comamonas aquatica.

Antimicrobial resistance, especially carbapenem resistance, poses a serious threat to global public health. Here, a carbapenem-resistant Comamonas aquatica isolate SCLZS63 was recovered from hospital sewage. Whole-genome sequencing showed that SCLZS63 has a 4,048,791-bp circular chromosome and three plasmids.

Establishment and application of multiplex real-time PCR for simultaneous detection of four viruses associated with porcine reproductive failure.

Many pathogens cause reproductive failure in sows suffering a broad spectrum of sequelae, including abortions, stillbirth, mummification, embryonic death, and infertility. Although various detection methods, such as polymerase chain reaction (PCR) and real-time PCR, have been widely used for molecular diagnosis, mainly for a single pathogen. In this study, we developed a multiplex real-time PCR method for the simultaneous detection of porcine circovirus type 2 (PCV2), porcine circovirus type 3 (PCV3), porcine parvovirus (PPV) and pseudorabies virus (PRV) associated with porcine reproductive failure.

Hormesis Responses of Growth and Photosynthetic Characteristics in Thunb. to Cadmium Stress: Whether Electric Field Can Improve or Not?

"Hormesis" is considered a dose-response phenomenon mainly observed at hyperaccumulator plants under heavy metals stress. In this study, the effects of electric fields on hormesis responses in Thunb. under cadmium (Cd) treatments were investigated by assessing the plant growth and photosynthetic characteristics.

Function analysis of choline binding domains (CBDs) of LytA, LytC and CbpD in biofilm formation of Streptococcus pneumoniae.

Biofilm formation is an important strategy for the colonization of Streptococcus pneumoniae, which can increase the capacity to evade antibiotic and host immune stress. Extracellular choline-binding proteins (CBPs) are required for successful biofilm formation, but the function of extracellular CBPs in the process of biofilm formation is not fully understood. In this study, we tend to analyze the functions of LytA, LytC and CbpD in biofilm formation by in vitro studies with their choline-binding domains (CBDs).

Recent Advances of the Confinement Effects Boosting Electrochemical CO Reduction.

Powered by clean and renewable energy, electrocatalytic CO reduction reaction (CO RR) to chemical feedstocks is an effective way to mitigate the greenhouse effect and artificially close the carbon cycle. However, the performance of electrocatalytic CO RR was impeded by the strong thermodynamic stability of CO molecules and the high susceptibility to hydrogen evolution reaction (HER) in aqueous phase systems. Moreover, the numerous reaction intermediates formed at very near potentials lead to poor selectivity of reaction products, further preventing the industrialization of CO RR.

Genomic characteristics of clinical multidrug-resistant isolates from a tertiary care hospital in southwest China.

Multidrug-resistant (MDR) , especially those strains producing extended-spectrum β-lactamases (ESBL) and carbapenemases, represents a major public health concern. In the present work, we characterized 27 MDR clinical isolates, including 23 , three , and one , by whole-genome analysis. Among the 27 isolates analyzed, SXT/R391 ICEs were detected in 14 strains, and the complete sequences of nine ICEs were obtained.