Real-world clinical experience with serum MOG and AQP4 antibody testing by live versus fixed cell-based assay.

Objective: To assess the real-world performance of a live (LCBA) versus a fixed (FCBA) cell-based assay for the detection of serum antibodies directed against myelin oligodendrocyte glycoprotein (MOG-IgG) and aquaporin-4 (AQP4-IgG).

Methods: This was a retrospective study of patients evaluated at a single tertiary academic referral center, with serum testing performed clinically for AQP4-IgG and/or MOG-IgG by FCBA and LCBA on the same day. Additionally, frozen banked sera from the same day for patients tested only by one assay were retrieved and tested by the other assay.

A Comparison of gel (Hydragel 30) and capillary (Capillarys III Tera) electrophoresis for the characterization of human serum proteins.

Objectives: To compare gel (Hydrasys 2 from Sebia) and capillary (Capillarys III Tera, Sebia) electrophoresis for the characterization of human serum proteins.

Design And Methods: 304 sera tested by gel electrophoresis during 8 routine laboratory days were concurrently tested by capillary electrophoresis. Gels were read by an experienced medical technologist while capillary profiles by a Sebia representative and the same technologist.