Publications by authors named "Lu-kang Zheng"
Objective: To assess the effect of benzene on sperm DNA.
Methods: Twenty-seven workers exposed to benzene for over two years were included in the experiment group and 35 unexposed ones were chosen as controls. Damage by benzene on sperm DNA was detected by modified single cell gel electrophoresis (SCGE).
[Detection of numerical aberrations of chromosomes 7 and 8 in sperms of workers exposed to benzene series by two-color fluorescence in situ hybridization].
Zhonghua Yi Xue Yi Chuan Xue Za Zhi
August 2004
Objective: To investigate the frequency of numerical aberrations for chromosomes 7 and 8 in the sperms of workers exposed to benzene series.
Methods: Numerical aberrations in the sperms of workers were detected by two-color fluorescence in situ hybridization with biotin labeled chromosome 7 probe (D7Z1) and digoxingenin labeled chromosome 8 probe (D8Z1).
Results: The time-weight average air concentration (TWA) of benzene in the workshop was 42.
Article Synopsis
- The study utilized multi-color FISH to analyze sperm from benzene-exposed workers, revealing higher frequencies of numerical and structural chromosome aberrations compared to controls.
- Benzene exposure levels in the workplace (TWA: 42.29 mg/m3) significantly exceeded national safety limits (6 mg/m3), and higher urinary concentrations of trans, trans-muconic acid in the exposed group were observed.
- Statistically significant increases were found in disomic and nullisomic sperm frequencies for chromosomes 1 and 18 in benzene-exposed workers, alongside increased rates of terminal and centromeric structural aberrations on chromosome 1.
To explore acrylonitrile (ACN)-induced DNA strand breakage and sex chromosome aneuploidy in human spermatozoa, semen parameters were examined among 30 acrylonitrile-exposed workers according to WHO laboratory manual for the examination of human sperm. DNA strand breakage of sperm cells was investigated among 30 ACN-exposed workers using single cell gel electrophoresis (SCGE). The frequency of sex chromosome aneuploidy in sperm cells was analyzed among nine ACN-exposed workers using fluorescence in situ hybridization (FISH).
View Article and Find Full Text PDFObjectives: To assess the effect of freezing on the sperm DNA.
Methods: To assess the sperm DNA preserved at -80 degrees C by using the single cell gel electrophoresis (SCGE).
Results: There was no statistical difference on the time factor by analysis of variance (ANOVA).