Functional and pathological recovery after spinal cord injury (SCI) is often incomplete due to the limited regenerative capacity of the central nervous system (CNS), which is further impaired by several mechanisms that sustain tissue damage. Among these, the chronic activation of immune cells can cause a persistent state of local CNS inflammation and damage. However, the mechanisms that sustain this persistent maladaptive immune response in SCI have not been fully clarified yet.
View Article and Find Full Text PDFMacrophages and microglia play important roles in chronic neuroinflammation following spinal cord injury (SCI). Although macrophages and microglia have similar functions, their phagocytic and homeostatic abilities differ. It is difficult to distinguish between these two populations , but single-cell analysis can improve our understanding of their identity and heterogeneity.
View Article and Find Full Text PDFUranium nitride-oxide cations [NUO] and their complexes with equatorial N ligands, [NUO·(N)] (n = 1-7), were synthesized in the gas phase. Mass-selected infrared photodissociation spectroscopy and quantum chemical calculations confirm [NUO·(N)] to be a sterically fully coordinated cation, with electronic singlet ground state of A, linear [NUO] core, and C structure. The presence of short N-U bond distances and high stretching modes, with slightly elongated U-O bond distances and lowered stretching modes, is rationalized by attributing them to cooperative covalent and dative [ǀN≡U≡Oǀ] triple bonds.
View Article and Find Full Text PDFBackground: Intestinal dysbiosis has been shown to be associated with the pathogenesis of alcoholic liver disease (ALD), which includes changes in the microbiota composition and bacterial overgrowth, but an effective microbe-based therapy is lacking. () CGMCC 7049 is a newly isolated strain of probiotic that has been shown to be resistant to ethanol and bile salts. However, further studies are needed to determine whether exerts a protective effect on ALD and to elucidate the potential mechanism.
View Article and Find Full Text PDF() functions as a probiotic in animals, but the underlying mechanisms remain unclear. We aim to evaluate the protective effects and definite mechanism by which orally administered prevents D-galactosamine (D-GalN)-induced liver injury in rats. Twenty-one Sprague-Dawley rats were equally assigned into three groups ( = 7 animals per group).
View Article and Find Full Text PDFPathological classification through transmission electron microscopy (TEM) is essential for the diagnosis of certain nephropathy, and the changes of thickness in glomerular basement membrane (GBM) and presence of immune complex deposits in GBM are often used as diagnostic criteria. The automatic segmentation of the GBM on TEM images by computerized technology can provide clinicians with clear information about glomerular ultrastructural lesions. The GBM region on the TEM image is not only complicated and changeable in shape but also has a low contrast and wide distribution of grayscale.
View Article and Find Full Text PDFNan Fang Yi Ke Da Xue Xue Bao
August 2011
Objective: To observe the ultracytochemical localization of H(+)-adenosine triphosphatase (H(+)-ATPase) in the cell organelles.
Methods: The localization of H(+)-ATPase in the cell organelles was observed in the hepatocytes and renal cells of Wistar rats using routine ultracytochemical methods.
Results: H(+)-ATPase activities were observed on the lysosomal membrane and nuclear envelope of the hepatocytes and proximal tubule epithelial cells of the nephron in Wistar rats.
Di Yi Jun Yi Da Xue Xue Bao
March 2004
Objective: To clone the cDNA of the preprotein cDNA of human bone morphogenetic protein 12 (hBMP12).
Methods: Two primers were designed according to hBMP12 sequence reported in GenBank. The hBMP12 preprotein cDNA was obtained by reverse transcriptional (RT)-PCR from the mRNA extracted from human placenta, followed by cloning into pTARGETTM plasmid and sequence analysis of the plasmid pT(ARGE)T(TM)-BMP12.
Objective: To observe the morphological changes of collagen fibrils during the formation of autogenous tendon induced by human hair keratin (HHK) artificial tendon.
Methods: Rabbit models of injured tendon were established in which implantation of HHK artificial tendon was performed to observe the formation of autogenous tendon under light microscope and electron microscope at 3, 6, 9, 12 and 16 weeks after HHK implantation.
Results And Conclusion: During autogenous tendon formation induced by HHK artificial tendon, the tendon cells of the impaired end of the tendon and beneath the tendon membrane dedifferentiate and are capable of collagen secretion, followed by the formation of typesI,II and III collagen fibrils, and eventually, the majority of the tendon cells disappear with the collagenization of the tendon.
Di Yi Jun Yi Da Xue Xue Bao
January 2001
OBJECTIVE: To study the differentiation of human mesenchymal stem cells (MSCs) derived from human fetal bone marrow and observe its telomerase activity. METHODS: MSCs were isolated from fetal bone marrow of the femur followed by cell culture and amplification. In situ hybridization was employed to detect telomerase activity in the MSCs.
View Article and Find Full Text PDFDi Yi Jun Yi Da Xue Xue Bao
October 2002
Objective: To observe the in vivo degradation process of human hair keratin (HHK) scaffold after implantation in rabbits.
Methods: Seven New Zealand rabbits were divided into 4 groups including a control group and 3 operation groups. HHK scaffold was implanted, after partial resection of the skeletal muscles, in rabbits of the 3 operation groups, followed by observation 1, 3, and 6 weeks later respectively.