[Construction of RNA interfering lentiviral vector of occludin and its role in tight junctions].

Objective: To investigate the role of occludin in tight junction (TJ) in vitro.

Methods: We constructed RNA interfering lentiviral vectors and transfected them into TM4 cells. Then we detected their inhibitory effect on occuldin by RT-PCR and Western blot and analyzed the role of occuldin in TJ using an in vitro TJ cell model.

[SP1 identified as a transcription factor of miR-122-5p].

Objective: To study the transcription factors of the spermatogenesis-related promoter mir-122-5p.

Methods: SP1 and GATA4 were predicted as the possible transcription factors of the mir-122-5p promoter by bioinformatics analysis, followed by construction of the double luciferase pGL3-mir-122-5p promoter vector, pcDNA3.1 (+) -SP1 expression vector and pcDNA3.