A novel strategy to control Pseudomonas syringae through inhibition of type III secretion system.

Pseudomonas syringae (P. syringae) is a highly prevalent Gram-negative pathogen with over 60 pathogenic variants that cause yield losses of up to 80% in various crops. Traditional control methods mainly involve the application of antibiotics to inactivate pathogenic bacteria, but large-scale application of antibiotics has led to the development of bacterial resistance.

To Explore the Molecular Mechanism of Acupuncture Alleviating Inflammation and Treating Obesity Based on Text Mining.

Objective: To explore the related mechanism of acupuncture affecting obesity by regulating inflammation using bioinformatics methods.

Methods: The genes related to obesity, inflammation, and acupuncture and inflammation were mined using GenCLiP 3, and the intersecting genes were extracted using Venn diagram. The DAVID database was employed for pathway enrichment analysis and functional annotation of coexpressed genes.

Discovery of a series of 5-phenyl-2-furan derivatives containing 1,3-thiazole moiety as potent Escherichia coli β-glucuronidase inhibitors.

Gut microbial β-glucuronidases have drawn much attention due to their role as a potential therapeutic target to alleviate some drugs or their metabolites-induced gastrointestinal toxicity. In this study, fifteen 5-phenyl-2-furan derivatives containing 1,3-thiazole moiety (1-15) were synthesized and evaluated for their inhibitory effects against Escherichia coli β-glucuronidase (EcGUS). Twelve of them showed satisfactory inhibition against EcGUS with IC values ranging from 0.

Meta-analysis of the effects of liming on soil pH and cadmium accumulation in crops.

Increasing cadmium (Cd) contamination in agricultural fields has resulted in a higher risk of Cd accumulation in the food chain. Lime addition can mitigate soil acidification and reduce Cd accumulation in crops cultured in Cd-contaminated soil. To determine key factors controlling the outcomes of liming in reducing Cd accumulation and enhancing soil pH, we performed a meta-analysis using previously published data from field and pot experiments.

Identification of critical genes associated with the development of asthma by co-expression modules construction.

Introduction: Asthma is a worldwide problem that is caused by complex underlying immune dysregulation. The identification of potential prognostic markers of asthma may provide information for treatment. The purpose of this study was to explore the key mechanisms involved in the development of asthma on the basis of microarray analysis.

[Nutrient Distribution of Overlying Water in Tidal Marshes in Five Estuaries and Bays of the Fuzhou Region in Autumn].

Overlying water from the tidal marshes in five estuaries and bays, namely, Xinghua Bay (Fuzhou Part), Fuqing Bay, Luoyuan Bay, Minjiang River Estuary, and Aojiang River Estuary of the Fuzhou region were collected in autumn of 2015, and the nitrogen and phosphorus nutrient concentration and other physical and chemical indicators of the overlying water were measured to discuss the reasons for the differences in the nutrient concentration of the overlying water in tidal marsh wetlands in different bays and estuaries. There were significant differences in the nitrogen and phosphorus nutrient concentrations of the overlying waters of the tidal marshes in the different bays and estuaries (<0.05).

Endothelial cell-specific anticoagulation reduces inflammation in a mouse model of acute lung injury.

Tissue factor (TF)-dependent coagulation contributes to lung inflammation and the pathogenesis of acute lung injury (ALI). In this study, we explored the roles of targeted endothelial anticoagulation in ALI using two strains of transgenic mice expressing either a membrane-tethered human tissue factor pathway inhibitor (hTFPI) or hirudin fusion protein on CD31 cells, including vascular endothelial cells (ECs). ALI was induced by intratracheal injection of LPS, and after 24 h the expression of TF and protease-activated receptors (PARs) on EC in lungs were assessed, alongside the extent of inflammation and injury.

Aerosolized antibiotics for ventilator-associated pneumonia: a pairwise and Bayesian network meta-analysis.

Background: Aerosolized antibiotics have been proposed as a novel and promising treatment option for the treatment of ventilator-associated pneumonia (VAP). However, the optimum aerosolized antibiotics for VAP remain uncertain.

Methods: We included studies from two systematic reviews and searched PubMed, EMBASE, and Cochrane databases for other studies.

Efficacy and safety of anti-interleukin-5 therapy in patients with asthma: A pairwise and Bayesian network meta-analysis.

Introduction: Anti-interleukin-5 therapy has been proposed as a novel and promising treatment option in asthma treatment. However, the optimum monoclonal antibodies for asthma treatment remain uncertain.

Methods: We searched the PubMed, EMBASE and Cochrane databases from their inceptions to June 2018 for randomized controlled trials that reported pulmonary function, adverse events, Asthma Quality of Life Questionnaire (AQLQ) scores, and asthmatic exacerbations resulting from anti-interleukin-5 therapy in asthma patients.

The synergistic effect of esophageal squamous cell carcinoma KYSE150 cells and M2 macrophages on lymphatic endothelial cells.

This study aimed to investigate whether tumor-associated macrophages (TAMs) and esophageal squamous cell carcinoma (ESCC) cells could synergistically influence the generation of lymphatic vessels via the VEGF-C/VEGFR-3 signaling pathway and to address its mechanism. M2 macrophages were sorted with immunomagnetic beads and induced . VEGF-C siRNA plasmids were constructed and transfected into M2 macrophages and the ESCC cell line KYSE150.

[Clinical Analysis of 7 Children with Mature B-cell Acute Lymphoblastic Leukemia].

Objective: To evaluate the efficacy of CCCG-BNHL-2015 protocol in treatment of children with mature B-cell acute lymphoblastic leukemia (mature B-ALL).

Methods: Seven pediatric patients with newly diagnosed mature B-ALL were treated by CCCG-BNHL-2015 protocol (risk group R4) in Children's Hospital of Nanjing Medical University from November 2014 to January 2017.

Results: The median age of patients at initial diagnosis was 7.

[Diurnal Variations of Concentration of Porewater Dissolved CH and CO in a Brackish Marsh Dominated by and During Neap and Spring Tidal Days in the Minjiang River Estuary].

Understanding of diurnal variation of soil porewater dissolved CO and CH concentration plays an important role in revealing carbon cycling in estuarine wetlands.During neap and spring tidal days in April and September 2010,the diurnal variations of soil porewater dissolved CO and CH concentrations and main impacting factors in a brackish marsh ecosystem dominated by two species of (brevifolius) and (common reed) were determined in the ShanYutan in the Min River estuary,southeast China.The results showed that:① the soil porewater dissolved CH concentration during the spring and neap tidal days in April and September ranged from 88.

[Characterization of free and bound organochlorine pesticides in four soil/sediments of the Pearl River Delta, China].

Free and bound residues are the main form of organochlorine pesticides (OCPs) in soils/sediments. Four surface soil/sediments from the Pearl River Delta, China, were selected to characterize free and bound OCPs in different fractions of organic matters in the samples through alka-extraction, HCl/HF demineralization and organic solvent extraction. Total OCPs concentrations of samples were 20.

[Expression of HSP70 mRNA and MDR1 mRNA in K562 cells induced by heat shock and ADM].

This study was purpose to investigate the expression levels of HSP70 and MDR1 genes under heat shock and/or adriamycin (ADM) chemotherapy stimulation. The K562 cells were bathed in water at 43 degrees C for 1 hour, then the heat-treated K562 cells were collected and were cultured at 37 degrees C. The expression of HSP70 was assayed by immunocytochemistry, the growth suppression rate of K562 cells was detected by MTT assay, the function of P-gp and the expressions of HSP70 mRNA, MDR1 mRNA were detected by flow cytometry and real-time quantitative PCR (RT-PCR) respectively.