[Tumor necrosis factor-alpha upregulates the protein levels of adipose differentiation-related protein in rat differentiated adipocytes].

Objective: To investigate the effect and basis of tumor necrosis factor-alpha (TNF-alpha) on the lipid droplet associating protein adipose differentiation-related protein(ADRP) in rat differentiated adipocytes.

Methods: Differentiated rat adipocytes derived from epididymal fat pads of Sprague-Dawley rats were incubated in the presence or absence of TNF-alpha (25 microg/L). Glycerol level released in the culture medium was determined by a colorimetric assay and served as an index of lipolysis.

[The mechanisms of stimulated lipolysis by high concentration of glucose in primary rat adipocytes].

Objective: To investigate the cellular mechanisms of adipocyte lipolytic response to a high concentration of glucose, which liberates free fatty acids (FFA) efflux from adipose cells to plasma.

Methods: Adipocytes were isolated from epididymal fat pads of Sprague-Dawley (SD) rats, and were incubated in the presence or absence of excess glucose (25 mmol/L). Glycerol released in the culture medium was determined by use of a colorimetric assay and served as an index of lipolysis.