Potent Antibody Response Elicited by a Third Booster Dose of Inactivated COVID-19 Vaccine in Healthy Subjects.

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccine has been used worldwide on a large scale because of its potent ability to contain the coronavirus disease 2019 (COVID-19) pandemic, and the antibody response induced by the vaccine needs to be elucidated. Thus, we conducted a prospective trial in healthy subjects to observe the antibody response after three doses of inactivated vaccines. Our results showed that neutralizing antibody (NAb) levels were significantly higher after the booster vaccination compared to the second, a 4.

Self-assembled nanoparticle with E protein domain III of DTMUV based on ferritin as carrier can induce a more comprehensive immune response and against DTMUV challenge in duck.

Duck Tembusu virus (DTMUV) causes severe reduction in egg production and neurological symptoms in ducklings. Vaccination is the primary measure used to prevent DTMUV infections. In this study, self-assembled nanoparticles with the E protein domain III of DTMUV, using ferritin as a carrier (EDⅢ-RFNp), were prepared using a prokaryotic expression system.

Self-Assembled Nanoparticles with E Protein Domains I and II of Duck Tembusu Virus Can Induce a More Comprehensive Immune Response Against the Duck Tembusu Virus Challenge.

Duck Tembusu virus (DTMUV) is a pathogenic flavivirus that causes a substantial drop in egg production and severe neurological disorders in domestic waterfowl. Self-assembled ferritin nanoparticles with E protein domains I and II (EDI-II) of DTMUV (EDI-II-RFNp) were prepared, and its morphology was observed. Two independent experiments were conducted.

The biomarkers related to immune infiltration to predict distant metastasis in breast cancer patients.

The development of distant metastasis (DM) results in poor prognosis of breast cancer (BC) patients, however, it is difficult to predict the risk of distant metastasis. Differentially expressed genes (DEGs) were screened out using GSE184717 and GSE183947. GSE20685 were randomly assigned to the training and the internal validation cohort.

Development of receptor binding domain-based double-antigen sandwich lateral flow immunoassay for the detection and evaluation of SARS-CoV-2 neutralizing antibody in clinical sera samples compared with the conventional virus neutralization test.

Vaccination is an effective strategy to fight COVID-19. However, the effectiveness of the vaccine varies among different populations in varying immune effects. Neutralizing antibody (NAb) level is an important indicator to evaluate the protective effect of immune response after vaccination.

Epidemiological survey and screening strategy for dengue virus in blood donors from Yunnan Province.

Background: Dengue virus (DENV) infection is increasingly common in southern China and can be transmitted through blood transfusion but is not currently part of donor screening throughout the region. We assessed DENV prevalence among donors at the Xishuangbanna Blood Center, Yunnan, to support development of DENV screening strategies.

Methods: Blood samples were collected randomly between June 2019 and August 2019.

Supramolecular self-assembly for designing non-centrosymmetric crystals based on Keggin polyoxometallates and crown ether.

We report the synthesis of five novel crystals [(4-BrAni+)(DB[18]crown-6)]2[SMo12O402-]·2CH3CN (1), [(4-BrAni+)(B[18]crown-6)]2[SMo12O402-]·CH3CN (2), [(4-BrAni+)(B[18]crown-6)]3[PMo12O402-]·2CH3CN (3), [(3-AP+)3(B[18]crown-6)2][PMo12O403-] (4) and [NBu4+][(3-AP2+)(DB[30]crown-10)][PMo12O403-]·CH3CN (5) (4-BrAni+ = 4-bromoanilinium; B[18]crown-6 = benzo[18]-crown-6; DB[18]crown-6 = dibenzo[18]-crown-6; DB[30]crown-10 = dibenzo[30]-crown-10; 3-AP+ = 3-aminopyridinium; 3-AP2+ = 3-ammoniumpyridinium; NBu4+ = tetrabutylammonium). In order to construct non-centrosymmetric crystals, the five crystals were designed using a method to gradually introduce asymmetry into the building units. Crystal 1 was constructed with a symmetric supramolecular cation (SPC) [(4-BrAni+)(DB[18]crown-6)], resulting in a P21/n space group.

Maintenance of cyclic GMP-AMP homeostasis by ENPP1 is involved in pseudorabies virus infection.

In a previous study, we demonstrated that porcine cyclic GMP-AMP (cGAMP) synthase (cGAS) catalyzes cGAMP production and is an important DNA sensor for the pseudorabies virus (PRV)-induced activation of interferon β (IFN-β). Ectonucleotide pyrophosphatase phosphodiesterase 1 (ENPP1) has recently been identified as the hydrolase of cGAMP in rodents, but its role in porcine cells is not clear. Our recent study demonstrated that porcine ENPP1 is responsible for the homeostasis of cGAMP and is critical for PRV infection.

Cholesterol 25-hydroxylase acts as a host restriction factor on pseudorabies virus replication.

Cholesterol 25-hydroxylase (CH25H) catalyses the production of 25-hydroxycholesterol (25HC) from cholesterol by adding a second hydroxyl group at position 25. The aim of this study was to examine the antiviral effect of CH25H on pseudorabies virus (PRV), a swine pathogen that can cause devastating disease and economic losses worldwide. The results showed that porcine ch25h was induced by either interferon or PRV infection.

[Comparison of green coffee beans volatiles chemical composition of Hainan main area].

Chemical component of Hainan green coffee beans was analyzed with solid phase microextraction-gas chromatography-mass spectrometry, and the discrepancy between two green coffee beans was differentiated through the spectrum database retrieval and retention index of compound characterization. The experimental results show that: the chemical composition of Wanning coffee beans and Chengmai coffee beans is basically the same. The quantity of analyzed compound in Wanning area coffee is 91, and in Chengmai area coffee is 106, the quantity of the same compound is 66, and the percent of the same component is 75.

Electrochemical reduction and voltammetric determination of metronidazole at a nanomaterial thin film coated glassy carbon electrode.

Simple and sensitive electrochemical method for the determination of metronidazole, based on a nanostructured film coated glassy carbon electrode (GCE), is described. Multi-walled carbon nanotubes (MWNT) was dispersed into water in the presence of a hydrophobic surfactant to give very stable and homogeneous MWNT suspension, and a MWNT-film coated GCE was achieved via evaporating solvent. Metronidazole yields a well-defined reduction peak whose potential is -0.

Meta-barium borate, II-BaB2O4, at 163 and 293 K.

The single-crystal X-ray diffraction structure analysis of an excellent non-linear optical material, viz. II-BaB2O4 or Ba3(B3O6)2, has been carried out at 163 and 293 K. The two sets of structural data are compared and indicate a significant shortening of the c axial length in the unit cell at 163 K, whereas the a and b axial lengths essentially do not change.

Electrocatalytic reduction of chloramphenicol at multiwall carbon nanotube-modified electrodes.

A multiwall carbon nanotube-modified glassy carbon (GC) electrode was employed for the investigation of chloramphenicol (CAP) reduction. Carbon nanotube coating can greatly lower the overpotential of the electrochemical reduction of CAP and promote the electrode reaction. CAP undergoes an irreversible reduction process in phosphate buffer by the modified electrode.

A multi-wall carbon nanotubes-dicetyl phosphate electrode for the determination of hypoxanthine in fish.

An enzymeless sensor based on a multi-walled carbon nanotubes-dicetyl phosphate (MWCNT-DCP) film modified vitreous carbon electrode was developed for the determination of hypoxanthine. The MWCNT-DCP film modified electrode showed a remarkable enhancement effect on the oxidation peak current of hypoxanthine. Under the optimized conditions, the oxidation peak current is proportional to the concentration of hypoxanthine over the range from 5.

A novel left-handed double helicate constructed from L-tartrate bridged molybdenum(vi) and gadolinium(iii) atoms.

The one-dimensional double helicate, [NH4][Mo2O4Gd(H2O)6(L-C4H2O6)2] x 4H2O (1), which was synthesized by the reaction of GdCl3, L-tartaric acid and ammonium molybdate in acidified water solution, is built up by two heft-handed single-helical chains, linked up further by eight-coordinated GdIII pieces in an enantiopure left-handed double helical configuration, of which each helix is formed by L-tartrate bridged six-coordinated MoVI atoms.

A novel compound with an interpenetrating 2D network structure constructed by [Mo5P2O23] and Ni-4,4'-bipyridine components: its synthesis, characterization and magnetic behaviour.

A Novel compound (Hbpy)2[[(H2O)3Ni(bpy)0.5Ni(bpy)(H2O)4]2[Mo5P2O23] [Ni((bpy)0.5)2(H2O)2][Mo5P2O23]].

Fluorometric determination of DNA using a new ruthenium complex Ru(bpy)2PIP(V) as a nucleic acid probe.

A new ligand, 2-phenyl(5-fluoro)imidazo[f]-1,10-phenanthroline (PIP(V)), and its coordination compounds, Ru(bpy)2PIP(V), were synthesized. The fluorescence spectrum of the interaction between Ru(bpy)2PIP(V) and DNA was studied, and a very strong fluorescence peak at a wavelength of 589 nm appeared. The optimum condition of analyzing DNA was decided.

A three-dimensional zeolite-like organic-inorganic hybrid material constructed from (CuMo2O8N)n double helical chains linked via [Cu(4,4'-bpy)]n fragments.

The three-dimensional microporous [Cu2Mo2O8(4,4'-bpy)]n.3nH2O 1 contains (CuMo2O8N) double helical chains, which are built up from (CuIIO4N) square pyramids linked by (MoVIO4) tetrahedra and further connected to each other by 4,4'-bpy coordinated (CuIIO5N); the study of the physical properties of 1 demonstrates it is a paramagnetic semiconductor and a zeolite material; the specific channeling cavities in 1 have potential adsorption activities, indicating that 1 might be an attractive functional microporous solid material.

Synthesis and Structural Characterization of an Unusual Oxygen-Linked Double Cubane-Like Cluster {[Mo(3)PbOS(3)](OAc)(2)(py)(3)(dtp)(2)O}(2).

The unusual oxygen-linked double cubane-like cluster compound {[Mo(3)PbOS(3)](OAc)(2)(py)(3)(dtp)(2)O}(2) (A) (dtp = S(2)P(OEt(2))(-), OAc = CH(3)COO(-), py = C(5)H(5)N) was obtained for the first time by the reaction of the mixed S/O-bridged cluster [Mo(3)(&mgr;(3)-O)(&mgr;-S)(3)(dtp)(4)H(2)O] (B) with Pb(OAc)(2).3H(2)O in the presence of excess CH(3)COOH and pyridine. It has been characterized by IR, Raman, UV-vis, and X-ray structure analysis.