[Clinical Analysis of B-CLPD with Cytopemia as the Predominant Characteristic].

Objective: To investigate the clinical characteristics and therapeutic responte of patients with B-CLPD mainly manifested as cytopenia, so as to deeply understand this disease.

Methods: The clinical data of 13 B-CLPD patients with hematocytopenia as main manifestation, and the absolute count of lymphocytes＜5×10/L, absence of hepatosplenic lymph-nodes and extramedullary invasion tin our department fron 2003 to 2018 were analyzed retrospectively. The clinical characteristics, therapeutic efficacy and adverse reactions of 3 patients were summarized.

Accurately cleavable goat β-lactoglobulin signal peptide efficiently guided translation of a recombinant human plasminogen activator in transgenic rabbit mammary gland.

Poor expression is the key factor hampering the large-scale application of transgenic animal mammary gland bioreactors. A very different approach would be to evaluate the secretion of recombinant proteins into milk in response to a cleavable signal peptide of highly secreted lactoproteins.We previously reported rabbits harboring mammary gland-specific expression vector containing a fusion cDNA (goat β-lactoglobulin (BLG) signal peptide and recombinant human plasminogen activator (rhPA) coding sequences) expressed rhPA in the milk, but we did not realize the signal peptide contributed to the high rhPA concentration and did not mention it at that time.

A cotton (Gossypium hirsutum) WRKY transcription factor (GhWRKY22) participates in regulating anther/pollen development.

Anther/pollen development is a highly programmed process in flowering plants. However, the molecular mechanism of regulating anther/pollen development is still largely unclear so far. Here, we report a cotton WRKY transcription factor (GhWRKY22) that functions in anther/pollen development.

A Model System for Studying the DNMT3A Hotspot Mutation (DNMT3A) Demonstrates a Causal Relationship between Its Dominant-Negative Effect and Leukemogenesis.

Mutation of DNA methyltransferase 3A at arginine 882 (DNMT3A) is prevalent in hematologic cancers and disorders. Recently, DNMT3A has been shown to have hypomorphic, dominant-negative, and/or gain-of-function effects on DNA methylation under different biological contexts. However, the causal role for such a multifaceted effect of DNMT3A in leukemogenesis remains undetermined.

'Double-muscling' and pelvic tilt phenomena in rabbits with the cystine-knot motif deficiency of myostatin on exon 3.

Gene mutations at different gene sites will produce totally different phenotypes or biological functions in gene-edited animals. An allelic series of mutations in the myostatin () gene can cause the 'double-muscling' phenotype. Although there have been many studies performed on -mutant animals, there have been few studies that have investigated the cystine-knot motif in exon 3 of in rabbits.

Characteristics of airborne opportunistic pathogenic bacteria during autumn and winter in Xi'an, China.

Bacteria are ubiquitous throughout the earth's lower atmosphere. Bacteria, especially pathogenic bacteria, play an important role in human health. The diversity, composition, and dynamics of airborne bacteria has been widely studied; however, the characteristics of pathogenic bacteria remain poorly understood.

Towards Detection of Glycoproteins Using Molecularly Imprinted Nanoparticles and Boronic Acid-Modified Fluorescent Probe.

Glycoproteins represent a group of important biomarkers for cancer and other life-threatening diseases. Selective detection of specific glycoproteins is an important step for early diagnosis. Traditional glycoprotein assays are mostly based on lectins, antibodies, and enzymes, biochemical reagents that are costly and require special cold chain storage and distribution.

Surface analysis of sheep menisci after meniscectomy via infrared attenuated total reflection spectroscopy.

Menisci are very important fibrocartilaginous tissue, which maintain biomechanical functions and physiological stabilization of knee joint. Meniscectomy is known as a surgery to recover partial functions from acute meniscus tears. However, the late consequences of total or partial meniscectomy include signs of osteoarthritis and even ligament instability.

Generation of hyperlipidemic rabbit models using multiple sgRNAs targeted CRISPR/Cas9 gene editing system.

Objective: To generate novel rabbit models with a large-fragment deletion of either LDL receptor (LDLR) and/or apolipoprotein (apoE) genes for the study of hyperlipidemic and atherosclerosis.

Methods: CRISPR/Cas9 system directed by a multiple sgRNAs system was used in rabbit embryos to edit their LDLR and apoE genes. The LDLR and apoE genes of founder rabbits were sequenced, and their plasma lipids and lipoprotein profiles on a normal chow diet were analyzed, western blotting was also performed to evaluate the expression of apolipoprotein.

Oxidation of methionine residues in human apolipoprotein A-I generates a potent pro-inflammatory molecule.

Amyloid deposits of apolipoprotein A-I (apoA-I) and inflammation are common in atherosclerotic arteries. In this study, we investigated the interplay between oxidation of apoA-I methionine residues (Met(O)-ApoA-I), a known amyloidogenic modification of apoA-I, and the inflammatory response of immune cells. Soluble pre-fibrillar Met(O)-ApoA-I, but not apoA-I, induced intracellular accumulation of pro-interleukin (IL)-1β and secretion of the pro-inflammatory cytokines tumor necrosis factor α (TNFα) and IL-6 in mouse bone marrow-derived macrophages (BMDMs) and human primary monocytes.

Characterization of bHLH/HLH genes that are involved in brassinosteroid (BR) signaling in fiber development of cotton (Gossypium hirsutum).

Background: Basic helix-loop-helix/helix-loop-helix (bHLH/HLH) transcription factors play important roles in plant development. Many reports have suggested that bHLH/HLH proteins participate in brassinosteroid (BR) hormone signaling pathways to promote cell elongation. Cotton fibers are single-cells and derived from seed surface.

A facile and general approach for the preparation of boronic acid-functionalized magnetic nanoparticles for the selective enrichment of glycoproteins.

Biomedical applications and biomarkers for early clinical diagnostics and the treatment of diseases demand efficient and selective enrichment platforms for glycoproteins. Thus, we herein report a facile and general approach for the preparation of boronic acid-functionalized magnetic nanoparticles for the selective enrichment of glycoproteins. More specifically, Fe3O4 magnetic nanoparticles were initially prepared via a solvothermal reaction, and core-shell-structured Fe3O4@SiO2 nanoparticles were obtained according to a sol-gel process.

Effects of sintering temperature on sensing properties of WO and Ag-WO electrode for NO sensor.

Pure WO and Ag-WO (mixed solid solutions Ag with WO) have been successfully synthesized by sol-gel method and the influences of calcination temperature on the particle size, morphology of the WO and Ag-WO nanoparticles were investigated. Powder X-ray diffraction results show that the hexagonal to monoclinic phase transition occurs at calcination temperature varying from 300°C to 500°C. SEM images show that calcination temperature plays an important role in controlling the particle size and morphology of the as-prepared WO and Ag-WO nanoparticles.

The Optimal Carbon Emission Reduction and Prices with Cap and Trade Mechanism and Competition.

More and more countries employ the Carbon Cap and Trade mechanism (CCT-mechanism) to stimulate the manufacturer to produce much more eco-friendly products. In this paper, we study how the CCT-mechanism affects competitive manufacturers' product design and pricing strategies. Assume that there are two competitive manufacturers; we give the optimal closed form solutions of the carbon emission reduction rates and retail prices in the Nash game model and the Stackelberg game model with CCT-mechanism, respectively.

ZFX Mediates Non-canonical Oncogenic Functions of the Androgen Receptor Splice Variant 7 in Castrate-Resistant Prostate Cancer.

Androgen receptor splice variant 7 (AR-V7) is crucial for prostate cancer progression and therapeutic resistance. We show that, independent of ligand, AR-V7 binds both androgen-responsive elements (AREs) and non-canonical sites distinct from full-length AR (AR-FL) targets. Consequently, AR-V7 not only recapitulates AR-FL's partial functions but also regulates an additional gene expression program uniquely via binding to gene promoters rather than ARE enhancers.

Spontaneous severe hypercholesterolemia and atherosclerosis lesions in rabbits with deficiency of low-density lipoprotein receptor (LDLR) on exon 7.

Rabbits (Oryctolagus cuniculus) have been the very frequently used as animal models in the study of human lipid metabolism and atherosclerosis, because they have similar lipoprotein metabolism to humans. Most of hyperlipidemia and atherosclerosis rabbit models are produced by feeding rabbits a high-cholesterol diet. Gene editing or knockout (KO) offered another means of producing rabbit models for study of the metabolism of lipids and lipoproteins.

Characteristics of ambient bioaerosols during haze episodes in China: A review.

Frequent low visibility, haze pollution caused by heavy fine particulate matter (PM) loading, has been entailing significant environmental issues and health risks in China since 2013. A substantial fraction of bioaerosols was observed in PM (1.5-15%) during haze periods with intensive pollution.

Analysis of human menisci degeneration via infrared attenuated total reflection spectroscopy.

Degeneration of human meniscal tissue induces impairment of normal knee functions, and is a highly relevant etiology of knee joint tears and osteoarthritis. Currently, the grading scale of meniscus degeneration is conventionally derived from evaluating meniscal morphology and histological staining. However, mid-infrared attenuated total reflectance (IR-ATR) spectroscopy is a particularly useful technique that may analyze the biomolecular composition at a sample surface, and provide information on the intra- and/or inter-molecular chemical bonds.

Inter-and intra-observer agreement on the judgment of toluidine blue staining for screening of oral potentially malignant disorders and oral cancer.

Objective: Toluidine blue staining (TBS) is used for screening of oral potentially malignant disorders (OPMDs) and oral cancer. Nevertheless, the judgment of TBS is based on the observer's personal experience and largely considered subjective. The aim of this study was to evaluate the inter- and intra-observer agreement on the judgment of TBS for screening of OPMDs and oral cancer.

A novel recombinant human plasminogen activator: Efficient expression and hereditary stability in transgenic goats and in vitro thrombolytic bioactivity in the milk of transgenic goats.

Background: Thromboses is a rapidly growing medical problem worldwide. Low-cost, high-scale production of thrombotic drugs is needed to meet the demand. The production of biomolecules in transgenic animals might help address this issue.

An estimate of rural exodus in China using location-aware data.

The rapidly developing economy and growing urbanization in China have created the largest rural-to-urban migration in human history. Thus, a comprehensive understanding of the pattern of rural flight and its prevalence and magnitude over the country is increasingly important for sociological and political concerns. Because of the limited availability of internal migration data, which was derived previously from the decennial population census and small-scale household survey, we could not obtain timely and consistent observations for rural depopulation dynamics across the whole country.

Optimizing Top- Multiclass SVM via Semismooth Newton Algorithm.

Top- performance has recently received increasing attention in large data categories. Advances, like a top- multiclass support vector machine (SVM), have consistently improved the top- accuracy. However, the key ingredient in the state-of-the-art optimization scheme based upon stochastic dual coordinate ascent relies on the sorting method, which yields complexity.

[Source Apportionment of Black Carbon Aerosol in the North Suburb of Nanjing].

Based on one year real-time measurements from a seven-wavelength Aethalometer combined with an Aethalometer model, the measured aerosol absorption coefficients at different wavelengths have been used to apportion the contribution of fossil fuel and biomass burning sources to the total black carbon (BC) mass concentration in the north suburb of Nanjing. Good consistency in the relationship between the Angstrom absorption exponent()and the ratio of BC from biomass burning sources to total BC (BB) was obtained during this period. The α was highest in winter and lowest in summer, which indicates the change in the source of the absorbing aerosols and their relative source strength.

[Concentration and Size Distribution Characteristics of Culturable Bioaerosols at Various Air Quality Levels During Fall and Winter in Xi'an, China].

The concentration and size distribution of culturable bacteria and fungi were studied in Xi'an city at various air quality levels. The culturable bioaerosols were collected by an Andersen bioaerosol aerosol sampler between Sept. 2014 and Jan.

Transgene-Assisted Genetic Screen Identifies and Novel Genes as Key Components of Antiviral RNA Interference in Caenorhabditis elegans.

RNA interference (RNAi) is a widespread antiviral mechanism triggered by virus-produced double-stranded RNAs (dsRNAs). In , antiviral RNAi involves a RIG-I-like RNA helicase, termed DRH-1 (dicer related RNA helicase 1), that is not required for classical RNAi triggered by artificial dsRNA. Currently, whether antiviral RNAi in involves novel factors that are dispensable for classical RNAi remains an open question.