Identification and characterization of a novel chromosome-encoded aminoglycoside -nucleotidyltransferase gene, in sp. TYF-12 isolated from the marine fish intestine.

Background: The mechanisms underlying the resistance of the genus to aminoglycosides are complex, which poses a challenge for the efficient treatment of infectious diseases caused by these pathogens. To help clinicians treat infections more effectively, a more comprehensive understanding of antibiotic resistance mechanisms is urgently needed.

Methods: Plates were streaked to isolate bacteria from the intestinal contents of fish.

APH(3')-Ie, an aminoglycoside-modifying enzyme discovered in a rabbit-derived isolate.

Background: Aminoglycoside-modifying enzymes (AMEs) play an essential role in bacterial resistance to aminoglycoside antimicrobials. With the development of sequencing techniques, more bacterial genomes have been sequenced, which has aided in the discovery of an increasing number of novel resistance mechanisms.

Methods: The bacterial species was identified by 16S rRNA gene homology and average nucleotide identity (ANI) analyses.

Identification and characterization of a novel 6'-N-aminoglycoside acetyltransferase AAC(6')-Va from a clinical isolate of .

Background: species have been identified as agents responsible for various diseases in both humans and animals. Multidrug-resistant strains pose a significant public health threat due to their emergence and spread in clinical settings and the environment. The aim of this study was to determine a novel resistance mechanism against aminoglycoside antimicrobials in a clinical isolate.

Identification and characterization of a novel β-lactamase gene, , from .

Background: is a genus of gram-negative bacteria that can act as opportunistic pathogens. Recent studies have revealed that some species of show inherent resistance to β-lactams, but the resistance mechanisms of have rarely been reported.

Method: The bacterium was isolated using standard laboratory procedures.

Identification and characterization of a novel chromosomal aminoglycoside 3'--phosphotransferase, APH(3')-Id, from DW18 isolated from the sewage of an animal farm.

Background: Aminoglycosides, as important clinical antimicrobials, are used as second-line drugs for treating multidrug-resistant tuberculosis or combined with β-lactam drugs for treating severe infections such as sepsis. Aminoglycoside-modifying enzyme (AME) is the most important mechanism of aminoglycoside resistance and deserves more attention.

Methods: The bacterium DW18 was isolated from the sewage of an animal farm using the conventional method.

, a novel AmpC gene identified from a isolate.

Background: species of the family are well-known plant pathogens and animal and human conditional pathogens. Due to the widespread and continuous use of antimicrobials, multidrug-resistant strains continue to emerge, making clinical treatment difficult; therefore, there is an increasing need to clarify the mechanisms of drug resistance.

Methods: A rabbit anal fecal sample was collected by a swab and the streak plate method was used to isolate single colonies.

Identification and characterization of a novel aminoglycoside -nucleotidyltransferase ANT(6)-If from PATH554.

Background: , a species of genus of the family , exists widely in environments and habitats in various plants and worms, and occasionally causes human infections. This work aimed to characterize the function of a novel aminoglycoside -nucleotidyltransferase resistance gene, designated , from a strain PATH554.

Methods: Molecular cloning, antimicrobial susceptibility testing, enzyme expression and purification, and kinetic analysis were used to validate the function of the novel gene.

ANT(9)-Ic, a Novel Chromosomally Encoded Aminoglycoside Nucleotidyltransferase from Brucella intermedia.

Aminoglycoside-modifying enzymes are among the most important mechanisms of resistance to aminoglycoside antibiotics, typically conferring high-level resistance by enzymatic drug inactivation. Previously, we isolated a multidrug-resistant Brucella intermedia strain ZJ499 from a cancer patient, and whole-genome sequencing revealed several putative novel aminoglycoside-modifying enzyme genes in this strain. Here, we report the characterization of one of them that encodes an intrinsic, chromosomal aminoglycoside nucleotidyltransferase designated ANT(9)-Ic, which shares only 33.

Class 1 integrons and multiple mobile genetic elements in clinical isolates of the complex from a tertiary hospital in eastern China.

Background: The emergence of highly drug-resistant , has become a major public health challenge. In this work, we aim to investigate the diversity of species and sequence types (STs) of clinical isolates and to characterize the prevalence and structure of class 1 integrons.

Methods: Based on the whole genome sequencing, species identification was performed by 16S rRNA gene homology and average nucleotide identity (ANI) analysis.

Functional characterization of a novel aminoglycoside phosphotransferase, APH(9)-Ic, and its variant from .

Background: The intrinsic resistance mechanism plays an essential role in the bacterial resistance to a variety of the antimicrobials. The aim of this study is to find the chromosome-encoded novel antimicrobial resistance gene in the clinical isolate.

Methods: The function of the predicted resistance gene was verified by gene cloning and antibiotic susceptibility test.

Characterization and Identification of a novel chromosome-encoded metallo-β-lactamase WUS-1 in P34.

In this study, we identified and characterized a novel chromosomally-encoded class B metallo-β-lactamase (MBL) gene designated in a carbapenem-resistant isolate P34 isolated from sewage discharged from an animal farm. Comparative analysis of the deduced amino acid sequence revealed that WUS-1 shares the highest amino acid similarities with the function-characterized MBLs MUS-1 (AAN63647.1; 70.

Characterization and identification of a novel chromosomal class C β-lactamase, LAQ-1, and comparative genomic analysis of a multidrug resistance plasmid in P13.

Introduction: , a bacterium usually isolated from natural environments, may cause human infections and has been suggested to be naturally resistant to second- and third-generation cephalosporins.

Methods: In this study, we determined the whole-genome sequence of an isolate, P13, isolated from animal farm sewage. On the basis of genome sequence analysis, susceptibility testing, molecular cloning, and enzyme kinetic parameter analysis, we identified a novel chromosome-encoded AmpC β-lactamase, LAQ-1.

AadA36, a novel chromosomal aminoglycoside nucleotidyltransferase from a clinical isolate of .

In this study, we characterized a novel chromosome-encoded aminoglycoside nucleotidyltransferase (ANT), AadA36, from the strain P14 isolated from the sputum specimen of a burn patient at a hospital in Wenzhou, China. Among the functionally characterized ANTs, AadA36 shared the highest amino acid sequence identity of 51.91% with AadA14.

Whole-Genome Sequencing-Based Species Classification, Multilocus Sequence Typing, and Antimicrobial Resistance Mechanism Analysis of the Enterobacter cloacae Complex in Southern China.

Members of the Enterobacter cloacae complex (ECC) are important opportunistic nosocomial pathogens that are associated with a great variety of infections. Due to limited data on the genome-based classification of species and investigation of resistance mechanisms, in this work, we collected 172 clinical ECC isolates between 2019 and 2020 from three hospitals in Zhejiang, China and performed a retrospective whole-genome sequencing to analyze their population structure and drug resistance mechanisms. Of the 172 ECC isolates, 160 belonged to 9 classified species, and 12 belonged to unclassified species based on ANI analysis.

Identification of a novel aminoglycoside -nucleotidyltransferase AadA33 in .

A novel chromosome-encoded aminoglycoside -nucleotidyltransferase AadA33 was identified in strain P13. The AadA33 shares the highest amino acid identity of 51.28% with the function characterized AadA31.

Molecular characterization of florfenicol and oxazolidinone resistance in isolates from animals in China.

Florfenicol is widely used for the treatment of bacterial infections in domestic animals. The aim of this study was to analyze the molecular mechanisms of florfenicol and oxazolidinone resistance in isolates from anal feces of domestic animals. The minimum inhibitory concentration (MIC) levels were determined by the agar dilution method.

N-glycosylation stabilizes MerTK and promotes hepatocellular carcinoma tumor growth.

Despite the evidences of elevated expression of Mer tyrosine kinase (MerTK) in multiple human cancers, mechanisms underlying the oncogenic roles of MerTK in hepatocellular carcinoma (HCC) remains undefined. We explored the functional effects of MerTK and N-Glycosylated MerTK on HCC cell survival and tumor growth. Here, we show that MerTK ablation increases reactive oxygen species (ROS) production and promotes the switching from glycolytic metabolism to oxidative phosphorylation in HCC cells, thus suppressing HCC cell proliferation and tumor growth.

Molecular Mechanism of the β-Lactamase Mediated β-Lactam Antibiotic Resistance of Isolated From a Chinese Teaching Hospital.

can cause infections in the blood, lungs (pneumonia), or other parts of the body after surgery. To investigate the molecular characteristics of β-lactam antibiotic resistance of isolated from a hospital population between 2015 and 2017, in this study, the antimicrobial susceptibility and the resistance gene profile of the bacteria were determined. The Pulsed-field gel electrophoresis (PFGE) was used to characterize the clonal relatedness and sequencing and comparative genomic analysis were performed to analyze the structure of the resistance gene-related sequences.

Ciclopirox targets cellular bioenergetics and activates ER stress to induce apoptosis in non-small cell lung cancer cells.

Background: Lung cancer remains a major cause of cancer-related mortality throughout the world at present. Repositioning of existing drugs for other diseases is a promising strategy for cancer therapies, which may rapidly advance potentially promising agents into clinical trials and cut down the cost of drug development. Ciclopirox (CPX), an iron chelator commonly used to treat fungal infections, which has recently been shown to have antitumor activity against a variety of cancers including both solid tumors and hematological malignancies in vitro and in vivo.

Characterization of a Novel Chromosome-Encoded AmpC β-Lactamase Gene, , in an Isolate of a Newly Classified Species, A20, From Animal Farm Sewage.

In this work, we characterized a novel chromosome-encoded AmpC β-lactamase gene, , in an isolate of a newly classified species designated A20, which was isolated from sewage discharged from an animal farm in Wenzhou, China. Susceptibility testing, molecular cloning, and enzyme kinetic parameter analysis were performed to determine the function and enzymatic properties of the β-lactamase. Sequencing and comparative genomic analysis were conducted to clarify the phylogenetic relationship and genetic context of the gene.

Identification and Characterization of a Novel Chromosomal Aminoglycoside 2'--Acetyltransferase, AAC(2')-If, From an Isolate of a Novel Species, R33.

Multidrug-resistant bacteria from different sources have been steadily emerging, and an increasing number of resistance mechanisms are being uncovered. In this work, we characterized a novel resistance gene named from an isolate of a novel species, R33 (CCTCC AB 2021339). Susceptibility testing and enzyme kinetic parameter analysis were conducted to determine the function of the aminoglycoside 2'--acetyltransferase.

Identification and Characterization of a Novel Aminoglycoside 3''-Nucleotidyltransferase, ANT(3'')-IId, From .

A novel plasmid-encoded aminoglycoside 3''-nucleotidyltransferase ANT(3")-IId, was discovered in strain H7 isolated from a chick on an animal farm in Wenzhou, China. The whole-genome of H7 consisted of one chromosome and five plasmids (pH7-250, pH7-108, pH7-68, pH7-48, and pH7-11). was identified as being encoded on pH7-250, sharing the highest amino acid identity of 50.

Identification of Three Clf-Sdr Subfamily Proteins in , and Comparative Genomics Analysis of a Locus Encoding CWA Proteins in Species.

Coagulase-negative is an opportunistic pathogen that is capable of causing several infections, especially in patients with indwelling medical devices. Here, we determined the complete genome sequence of a clinical strain isolated from the blood culture of a 1-year-old nursling patient with acute upper respiratory infection. Genome-wide phylogenetic analysis confirmed the phylogenetic relationships between and other species.

Identification and characteristics of a novel aminoglycoside phosphotransferase, APH(3')-IId, from an MDR clinical isolate of Brucella intermedia.

Objectives: To describe a novel chromosomal aminoglycoside phosphotransferase named APH(3')-IId identified in an MDR Brucella intermedia ZJ499 isolate from a cancer patient.

Methods: Species identity was determined by PCR and MALDI-TOF MS analysis. WGS was performed to determine the genetic elements conferring antimicrobial resistance.