A novel method to assess antibody-dependent cell-mediated cytotoxicity against influenza A virus M2 in immunized murine models.

The matrix protein 2 (M2) is a preferred target for developing a universal vaccine against the influenza A virus (IAV). This study aimed to develop a method for assessing antibody-dependent cell-mediated cytotoxicity (ADCC) associated with M2-based immunization in mice. We first established a stable cell line derived from mouse lymphoma cells (YAC-1) expressing M2 of H3N2.

Effect of Salt Reduction Interventions in Lowering Blood Pressure and Salt Intake in Zhejiang Province, China, 2017-2021: A Randomized Controlled Trial.

Background: Addressing high-salt diets in China through interventions can significantly reduce blood pressure (BP) and the associated health risks.

Objective: This study aims to evaluate the effectiveness of a comprehensive salt reduction intervention implemented across counties in Zhejiang Province, focusing on system establishment, extensive publicity, and targeted population interventions.

Methods: The Salt Reduction and Hypertension Prevention Project was initiated in Zhejiang Province.

The Incorporation of Nanoconfined Poly(ionic liquid)s with Two-Dimensional Covalent Organic Frameworks to Enhance Proton Conduction.

Covalent organic frameworks (COFs) hold promising potential as high-temperature proton conductors due to their highly ordered nanostructures and high specific surface areas. However, due to their limited functional groups and poor membrane-engineering properties, finding practical applications for COF-based proton-conducting materials still remains challenging. Herein, we proposed a universal strategy to fabricate proton-conducting composite membranes by the incorporation of sulfonic acid-bearing COFs and zwitterionic poly(ionic liquid)s (PILs) via in situ polymerization.

Heterogeneous cellular responses to hyperthermia support combined intraperitoneal hyperthermic immunotherapy for ovarian cancer mouse models.

The benefit of hyperthermic intraperitoneal chemotherapy (HIPEC) in ovarian cancer remains controversial, hindering the development of rational combination therapies based on hyperthermia (HT). This study reports the preliminary results of the neoadjuvant HIPEC (NHIPEC) trial (ChiCTR2000038173), demonstrating enhanced tumor response in high-grade serous ovarian cancer with NHIPEC. Through single-cell RNA sequencing analysis, we identified both homogeneous and heterogeneous cellular responses to HT within the tumor and microenvironment.

The correlation of starch composition, physicochemical and structural properties of different sorghum grains.

The composition, structure, and physicochemical properties of starch in sorghum grains greatly influence the processing and quality of the final products. In this study, 19 sorghum lines were examined to analyze various starch-related characteristics. Correlation analysis of these key traits, revealed a significant correlation between amylose and amylopectin content.

Analyzing corneal biomechanical response in orthokeratology with differing back optic zone diameter: A comparative finite element study.

Purpose: To investigate the impact of orthokeratology (ortho-k) lenses with different back optic zone diameter (BOZD) on the biomechanical response of the cornea, focusing on contact pressure, displacement, and stress distribution.

Method: Finite element models were developed to simulate varying corneal curvatures (39.0D, 42.

Comparison of Angle Kappa Measurements: Repeatability of Sirius, Keratron, and Pentacam and Agreement With Amaris 750S.

Purpose: To evaluate the repeatability of angle kappa measurements obtained from the Pentacam (Oculus Optikgeräte GmbH), Sirius (Costruzione Strumenti Oftalmici), and Keratron Scout (BVI), and to assess the agreement with those obtained from the Amaris 750S (SCHWIND eye-tech-solutions).

Methods: This study included 110 patients scheduled to undergo transepithelial photorefractive keratectomy or femtosecond laser-assisted laser in situ keratomileusis. Before surgery, each patient underwent angle kappa measurement using three instruments: Pentacam, Sirius, and Keratron.

The Peripheral Defocus Designed Spectacle Lenses Might Increase Astigmatism in Myopic Children.

Purpose: This study aims to explore the impact of wearing peripheral defocus spectacle lenses (PDSL) on cylindrical refractive error (CYL) in myopic children.

Methods: This study included 1057 myopic children and divided the participants into three groups: the HAL group (spectacle lens with highly aspherical lenslets), the MPV group (spectacle lens based on manipulating peripheral vision), and a control group (without myopia control interventions). The study analyzed the effect of wearing PDSL on changes in spherical equivalent refraction, CYL, and corneal astigmatism (CA).

Ferroptosis and cuproptosis in periodontitis: recent biological insights and therapeutic advances.

Periodontitis is a significant global public health issue associated with the onset and progression of various systemic diseases, thereby requiring additional research and clinical attention. Although ferroptosis and cuproptosis have emerged as significant areas of research in the medical field, their precise roles in the pathogenesis of periodontitis remain unclear. We aim to systematically summarize the current research on ferroptosis and cuproptosis in periodontal disease and investigate the roles of glutathione pathway and autophagy pathway in connecting ferroptosis and cuproptosis during periodontitis.

Fluorine-Nitrogen Codoped Carbon Dots for Visualization Imaging of Nucleic Acids via Two-Photon Fluorescence Lifetime Microscopy.

Fluorescence imaging is a key tool for visualizing the morphology and dynamics of nucleic acids (DNA and RNA) in living cells to understand their role in regulating the growth, development, and reproduction of organisms. However, effective probes capable of simultaneously targeting both DNA and RNA, as well as tools for analyzing their distribution and relative ratios in organisms, are currently lacking. Therefore, fluorine-nitrogen codoped carbon dots with two-photon absorption (F-NCDs) were synthesized by the hydrothermal method and exhibited stable fluorescence, good biocompatibility, and a fluorescence lifetime sensitive to nucleic acids (DNA and RNA).

Prognostic Factors and Nomogram for Infections in Intensive Care Unit.

Purpose: infections pose a significant threat to public health with high morbidity and mortality rates. The early identification of risk factors for mortality and accurate prognostic evaluation are important. Therefore, we aimed to identify the risk factors for mortality in patients with infections and develop a nomogram model for prognosis.

Performance evolution of the Nano Boron nitride enhanced bone cement composites.

Bone cement is a research hotspot and has been partially applied in the field of bone repair thanks to the good mechanical, physical and antibacterial properties. However, the easy wear and high temperature during curing characteristics would cause surrounding tissue necrosis, which seriously limits the wider application to some extent. In this work, the hexagonal boron nitride (h-BN) nano flakes were optimized to enhance the bone cement matrix (PMMA) via mechanical doping.

Robust and blind video watermarking against online sharing platforms.

In recent years, with the access of high-speed broadband, the number of videos on online sharing platforms has increased sharply, and the copyright protection of videos has been widely concerned. However, existing block-based watermarking methods have high sensitivity under geometric deformation and cannot accurately locate the watermark position, which leads to the failure of watermark extraction. Therefore, a robust and blind video watermarking scheme to resist the online sharing platform is proposed.

Prevalence of asymptomatic malaria in high- and low-transmission areas of Tanzania: The role of asymptomatic carriers in malaria persistence and the need for targeted surveillance and control efforts.

As many countries implement different programs aimed at eliminating malaria, attention should be given to asymptomatic carriers that may interrupt the progress. This was a community-based cross-sectional study conducted in Tanzania from December 2022 to July 2023 within 4 villages from each of the 3 regions, Geita and Kigoma, which are high malaria transmission, and Arusha, which is low transmission. Malaria was diagnosed in asymptomatic individuals aged 1 year and older using the malaria rapid diagnostic test and light microscope.

16S rDNA sequencing reveal synergistic effects of silkworm feces and earthworms on nutrient-poor soil microbial community structure and function in Guangxi.

This study evaluates the synergistic effects of silkworm feces and earthworms on nutrient-poor acidic red soils in Guangxi, China. Using 16S rDNA amplicon sequencing, soil samples from untreated fields, silkworm feces, earthworm gut contents, and soils treated with silkworm feces combined with three earthworm densities (50, 80, and 110 worms/kg) were compared. The earthworm gut microbiome increased in diversity in a density-dependent manner, while treated soils displayed enhanced microbial richness-with the 80 worms/kg treatment showing the highest diversity (605 genera).

The relationship between folic acid deficiency and preeclampsia-like phenotypes in rats.

Background: Pre-eclampsia is a significant contributor to maternal and neonatal morbidity and mortality. However, its etiology remains elusive. More and more studies have highlighted the potential involvement of folic acid metabolism in the development of pre-eclampsia.

Advanced oxidation process with hydrogen peroxide and sulfite for superfast degradation of micro-contaminants in drinking water.

Sulfite (S(IV))-based advanced oxidation processes (AOPs) have recently gained attention as viable alternatives to peroxosulfate-based AOPs due to their low toxicity and cost-effectiveness. Hydrogen peroxide (HO) is widely recognized as an effective and environmentally friendly oxidant in drinking water treatment. This study introduces a novel HO/S(IV) AOP based on the observation of over-stoichiometric consumption of S(IV) by HO.

Mechanisms Underlying Delayed loss of HBeAg and HBV DNA Following HBsAg Seroclearance in PEG-IFNα Treated Patients of chronic hepatitis B.

Background & Aims: A notable proportion of CHB patients undergoing PEG-IFNα based therapy experience lagged serum HBeAg and/or HBV DNA disappearance in patients achieving HBsAg loss. In this study, we explored the molecular mechanisms behind this clinical phenomenon, offering novel insights into the sustainability of chronic HBV infection.

Methods: Two independent clinical cohorts were enrolled to validate this phenomenon.

Regulatory role and therapeutic prospect of lactate modification in cancer.

Post-translational modifications (PTMs) of proteins refer to the process of adding chemical groups, sugars, or other molecules to specific residues of target proteins following their biosynthesis by ribosomes. PTMs play a crucial role in processes such as signal transduction, epigenetics, and disease development. Lactylation is a newly discovered PTM that, due to its close association with lactate-the end product of glycolytic metabolism-provides a new perspective on the connection between cellular metabolic reprogramming and epigenetic regulation.

Does the alternating timing of rTMS combined with soft-hand rehabilitation robot affect the recovery of hand function in patients after stroke? A study protocol for a multicentre randomised controlled trial.

Introduction: Combining repetitive transcranial magnetic stimulation (rTMS) with robotic training could result in more significant improvements in motor function than either treatment alone. The efficacy of this combination may depend on the sequencing of the interventions. However, few studies have explored the possibility of interleaving or alternating between the two treatment modalities within a single session or over a shorter time frame.

Domino volumetric metamaterial resonator for very-low-field MRI.

Background: Very-low-field magnetic resonance imaging (VLF-MRI) plays a significant role in medical imaging diagnosis due to its low cost and light weight. High-quality MR images are essential for accurate medical diagnosis. It is urgent to explore a low-cost, simple and convenient approach to boost the signal-to-noise ratio (SNR) of VLF-MRI system to make medical diagnosis more accurate.

Combined and mediating effects of remnant cholesterol and renal function on hypertension risk in Chinese middle-aged and elderly people.

Background: Emerging evidence indicates a potential correlation between remnant cholesterol (RC) and the development of vascular damage and hypertension. Nevertheless, the precise relationship between RC and hypertension in relation to renal function remains uncertain. The objective of this investigation was to employ a cohort design to evaluate the intricate correlation between RC and renal function in relation to hypertension.

Energy harvesting from clothing.

Seeking new energy sources, especially those with less environmental impact, has been a consistent effort in the field of energy harvesting. In this work, we present an innovative energy harvesting technique for collecting energy from clothing. In this convenient but powerful method, electrical energy is generated by simply sliding a zein film assembly on the surface of clothes by constructing zein/cloth direct current (DC) triboelectric nanogenerators (TENGs).

Risk assessment and mechanism of rock burst for deep soft coal roadways.

The occurrence of rock burst is related to the physical properties of coal-bearing strata. Under the same mining conditions, the excavation of soft coal seams at 1000 m or deeper has a lower risk of rock burst. The characteristics of rock burst in soft coal seams include a small impact influence range, large roadway deformation, and serious damage to roadway supporting components.

Novel Function of NUP153 in HNF4α Transcriptional Upregulation Contributes to Promoting HBV Replication.

Hepatitis B virus (HBV) infection remains a major public health problem, causing nearly one million deaths annually. Nucleoporin 153 (NUP153) is known to facilitate the nuclear entry of the human immunodeficiency virus (HIV) nucleocapsids, and recent studies suggest it also plays a role in HBV nucleocapsids nuclear import. We aimed to investigate the impact of NUP153 on HBV replication and its underlying mechanism.