Dose Characterization of the Investigational Anticancer Drug Tigilanol Tiglate (EBC-46) in the Local Treatment of Canine Mast Cell Tumors.

Mast cell tumor (MCT) is the most common cutaneous neoplasm in dogs and wide surgical resection is the current first-line treatment. However, recurrence is common and often requires more specialist and expensive therapies. Tigilanol tiglate is a novel small molecule drug delivered by intratumoral injection that is currently under development to provide a new option for treating MCT.

Evolution of endogenous retroviruses in the Suidae: evidence for different viral subpopulations in African and Eurasian host species.

Background: Porcine endogenous retroviruses (PERVs) represent remnants of an exogenous form that have become integrated in the domestic pig (Sus scrofa) genome. Although they are usually inactive, the capacity of γ1 ERVs to infect human cells in vitro has raised concerns about xenotransplantation because the viruses could cross the species barrier to humans. Here we have analyzed the evolution of γ1 ERVs in ten species of Suidae (suids, pigs and hogs) from Eurasia and Africa using DNA sequences for their coding domains (gag, pro/pol and env genes).

Distinctive differences in long terminal repeat sequences between γ1 endogenous retroviruses of African and Eurasian suid species.

Diversity of long terminal repeats (LTRs) from γ1 endogenous retroviruses (ERVs) was analysed by DNA sequencing in 10 species of the family Suidae (suids, pigs and hogs). Phylogenetic analysis separated LTR sequences into two groups which correlated approximately with either the previously described cluster I and III, or the clusters II, IV and V. Interestingly, a specific LTR exhibiting a novel molecular rearrangement was identified exclusively within African host species when compared to LTRs previously reported from known ERVs in the domestic pig (Sus scrofa).

Analysis of the non-recombining Y chromosome defines polymorphisms in domestic pig breeds: ancestral bases identified by comparative sequencing.

Sequences from 20 amplicons representing nine different loci and 11369bp from the short arm of the pig Y chromosome were compared using pools of DNA from different European and Chinese breeds. A total of 33 polymorphic sites were identified, including five indels and 28 single nucleotide polymorphisms (SNPs). Three high frequency SNPs within the coding regions of SRY were further analysed across 889 males representing 25 European and 25 Asian breeds or Lines, plus a European Line of Meishan.

Treatment of natural infestations of the biting louse (Werneckiella equi) on horses using triflumuron, a benzoylurea derivative insect growth regulator.

The horse biting louse (Werneckiella equi) is a common global equine ectoparasite. To our knowledge, benzoyl(phenyl)urea insecticides (triflumuron, diflubenzuron) commonly used as sheep lousicides, have not been evaluated for efficacy against W. equi.

Macrophage transcriptional responses following in vitro infection with a highly virulent African swine fever virus isolate.

We used a porcine microarray containing 2,880 cDNAs to investigate the response of macrophages to infection by a virulent African swine fever virus (ASFV) isolate, Malawi LIL20/1. One hundred twenty-five targets were found to be significantly altered at either or both 4 h and 16 h postinfection compared with targets after mock infection. These targets were assigned into three groups according to their temporal expression profiles.

Abolition? It's nothing personal. Interview by Nick Edwards.

The Commission for Patient and Public Involvement in Health was the victim of a 'political decision' and was not given the opportunity to prove itself, according to chief executive Steve Lowden. He also defended the 'courageous' decision to set up a network of not-for-profit forum support organisations, which some criticised for underfunding forums themselves.

Worldwide phylogeography of wild boar reveals multiple centers of pig domestication.

Mitochondrial DNA (mtDNA) sequences from 686 wild and domestic pig specimens place the origin of wild boar in island Southeast Asia (ISEA), where they dispersed across Eurasia. Previous morphological and genetic evidence suggested pig domestication took place in a limited number of locations (principally the Near East and Far East). In contrast, new genetic data reveal multiple centers of domestication across Eurasia and that European, rather than Near Eastern, wild boar are the principal source of modern European domestic pigs.

Novel herpesviruses of Suidae: indicators for a second genogroup of artiodactyl gammaherpesviruses.

Five novel herpesviruses were identified in suid species from Africa (common warthog, Phacochoerus africanus) and South-East Asia (bearded pig, Sus barbatus; babirusa, Babyrousa babyrussa) by detection and analysis of their DNA polymerase genes. Three of the novel species, P. africanus cytomegalovirus 1, P.

Genetic and physical mapping, expression analysis and partial sequence of porcine PER1.

The porcine PER1 gene was mapped to chromosome 12q1.4-->q1.5 using fluorescence in situ hybridisation.

Pathways of interstitial fluid and lymph flow in the liver acinus of the sheep and mouse.

In the acinus of the sheep and mouse liver, lymphatic vessels are restricted to the portal tracts. Vessels less than about 25 microm across form a network around portal venules, and are closely associated with the limiting plate of hepatocytes. The perisinusoidal space of Disse is continuous with the interstitial space of the portal tracts at the origin of the sinusoids.

Lymph pathways associated with three types of follicle structure found in gut-associated lymphoid tissue of horse ileum.

In the horse ileum, lacteals in the villi are continuous with prelymphatic intercellular channels and a plexus of lymphatic sinuses in the lamina propria that encircle the domes of the follicle/dome structures and proprial follicles. These sinuses may act as the major entry site for many of the lymphocytes migrating from gut-associated lymphoid tissue via the lymphatic system. Vessels from this plexus penetrate the muscularis mucosae and lymph flows into lymphatic vessels within the interfollicular tissue between the follicles of both follicle/dome structures and lymphoglandular complexes (LGCs).

Segmented filamentous bacteria associated with lymphoid tissues in the ileum of horses.

Segmented filamentous bacteria preferentially attached to the follicle-associated epithelium overlying the lymphoid tissue in samples of the terminal ileum from seven horses examined by scanning electron microscopy. The bacteria adhered to the apical membrane of the enterocytes by a holdfast segment. Each filament tended to be of uniform diameter, but the filaments ranged from 0.

Lymphoid tissues of the ileum in young horses: distribution, structure, and epithelium.

Lymphoid tissues in the ileum of young horses form raised plaques that are macroscopically visible from the mucosal surface. These are termed "ileal lymphoid patches". These patches are variable in size, shape and position within the ileal wall, occasionally lying along the site of mesenteric attachment.

Ileal Peyer's patches in pigs: intercellular and lymphatic pathways.

Background: The lymphatics of Peyer's patches disseminate immunological information from the gut and thus play a key role in protection of the body against environmental pathogens. The aim of this project was to describe the lymphatic pathways of these Peyer's patches in pigs, and the mucosal intercellular spaces which lead to these lymphatics.

Methods: Ileal tissue from living or freshly killed pigs was examined by light microscopy or electron microscopy, or was injected with Mercox (CL-2B, Japan Vilene Hospital, Tokyo) for scanning electron microscopy of corrosion casts.

Lymphatic drainage from the distal small intestine in sheep.

Lymphatic drainage from the wall of the distal small intestine, important especially in young sheep as a major site of gut-associated lymphoid tissue, begins with a series of longitudinally oriented subserosal vessels. These vessels convey lymph to the mesenteric border of the intestinal wall and unite to form larger vessels which course through the mesentery to the mesenteric lymph nodes. These nodes lie towards the periphery of a broad, fan-shaped mesentery, adjacent to major arteries and veins.

Lymph pathways associated with Peyer's patches in sheep.

Lymphatic drainage of the ileum associated with Peyer's patches begins with lymph entering a single lacteal of the villus, probably through intercellular flaps, which prevent retrograde flow to the interstitium. These lacteals are continuous with an interconnecting plexus of branching sinuses which surrounds the crypts and follicle domes in the lamina propria. Small vessels emanating from this plexus penetrate the muscularis mucosa, where lymph can either flow within septal vessels to the deep submucosa, or enter the follicular sinuses to move freely around follicles, and through gaps in septal walls.