Publications by authors named "Loukopoulos P"

Article Synopsis
  • Buruli ulcer (BU) is a neglected tropical disease caused by the bacterium Mycobacterium ulcerans, primarily affecting skin and subcutaneous tissue, with transmission dynamics varying by geographic area, particularly in endemic regions like Victoria, Australia.
  • In a study conducted in Melbourne and Geelong, researchers conducted necropsies and surveillance on possums, revealing that 77% of the 26 examined possums were common ringtails, with 9 showing cutaneous ulcers and 73% testing positive for M. ulcerans DNA, indicating a significant reservoir in these animals.
  • The findings suggest that possums may contract BU through bites from infected mosquitoes, environmental sources, or wounds, highlighting the need for a One Health approach to
View Article and Find Full Text PDF
Article Synopsis
  • Buruli ulcer, caused by the bacterium Mycobacterium ulcerans, affects the skin and tissues of various animals, including possums, and can lead to severe health issues and poor welfare.
  • Four common ringtail possums in Melbourne suffered from severe forms of the disease, resulting in extensive tissue damage, necessitating euthanasia due to the severity of their ulcerative lesions.
  • Findings suggest that Buruli ulcer significantly impairs possum mobility and social behaviors, with infected possums also potentially spreading the bacteria, highlighting the need for more research on the disease's impacts and potential interventions.
View Article and Find Full Text PDF

We characterized naturally occurring pigeon herpesvirus (PiHV; ) infection in domestic pigeons in California. We retrieved and analyzed 62 pathology reports produced between 1991 and 2014 at the California Animal Health and Food Safety Laboratory System. In 56 of the cases, the diagnosis of PiHV infection was established based on histopathology, either alone (44 cases) or combined with virus isolation (VI; 8), transmission electron microscopy (TEM; 3), or PCR (1); in the remaining 6 cases, the diagnosis was established based on VI (5 cases) or TEM (1) alone.

View Article and Find Full Text PDF

Aims: To investigate the relative differences in the perceptions and awareness of outbreaks of photosensitisation in Australian livestock stakeholders, including veterinarians, livestock traders and producers.

Methods: A questionnaire was developed and circulated to livestock veterinarians, producers and traders in Australia via email addresses obtained from public access sources. The questionnaire was designed to evaluate participants' awareness and perception of health, welfare, and production issues associated with outbreaks of photosensitisation and towards the incidence and importance of photosensitisation in livestock.

View Article and Find Full Text PDF

Australia has over 30 Panicum spp. (panic grass) including several non-native species that cause crop and pasture loss and hepatogenous photosensitisation in livestock. It is critical to correctly identify them at the species level to facilitate the development of appropriate management strategies for efficacious control of Panicum grasses in crops, fallows and pastures.

View Article and Find Full Text PDF

The sequencing of the canine genome, combined with additional genomic technologies, has created opportunities for research linking veterinary genomics with naturally occurring cancer in dogs. Also, as numerous canine cancers have features in common with human cancers, comparative studies can be performed to evaluate the use of cancers in dogs as models for human cancer. There have been several reviews of veterinary genomics but, to the best of our knowledge, there has been no comprehensive review of the literature of canine cancer genomics.

View Article and Find Full Text PDF

Using analytical chemistry techniques such as nuclear magnetic resonance (NMR) spectroscopy and liquid or gas chromatography-mass spectrometry (LC/GC-MS), metabolomics allows detection of most endogenous and exogenous metabolites in a biological sample. Metabolomics has a wide range of applications, and has been employed in nutrition science, toxicology, environmental studies, and systems biology. Metabolomics is particularly useful in biomedical science, and has been used for diagnostic laboratory testing, identifying targets for drug development, and monitoring drug metabolism, mode of action, and toxicity.

View Article and Find Full Text PDF

Background: Cervicothoracic vertebral subluxation in sheep presents as a postural and locomotor disorder, and has been described in several breeds in Australia and overseas. Cervical myopathy may also be present in these cases.

Case Report: A New South Wales sheep producer reported a postural and locomotor disorder with a low prevalence in his Poll Merino stud flock, affecting neonate, weaner and adult sheep.

View Article and Find Full Text PDF

The aim of this study was to examine the histopathological and immunohistochemical changes caused by natural and experimentally-induced infection in the respiratory system of chickens. To this end, three different studies were carried out. The first was a retrospective study of 82 field cases with respiratory disorders compatible with infection.

View Article and Find Full Text PDF

Background: Photosensitisation is a clinical condition occurring in both humans and animals that causes significant injury to affected individuals. In livestock, outbreaks of photosensitisation caused by ingestion of toxic plants are relatively common and can be associated with significant economic loss.

Objectives: The agents that are most commonly implicated in outbreaks of photosensitisation have not been formally investigated on a global scale.

View Article and Find Full Text PDF

Tongue worms utilise herbivorous mammals as intermediate hosts and reside in the nasopharynx of carnivores as their definitive hosts. A recent study in south eastern Australia showed an unexpectedly high infection (67%) of wild dogs with these parasites. The present study aimed at determining the pathogenicity of the parasite in both definitive (dog) and intermediate (cattle) hosts by histopathology.

View Article and Find Full Text PDF

Background: Primary photosensitisation (PS) subsequent to ingestion of the pasture legume Biserrula pelecinus L. (biserrula) has recently been confirmed in grazing livestock. Given the potential utility of this pasture species in challenging climates, a grazing trial was undertaken to examine if both varieties 'Casbah' and 'Mauro' were able to cause photosensitisation in livestock, and if this could be mitigated by grazing in winter, or in combination with other common pasture species.

View Article and Find Full Text PDF

Objectives: Second intention wound healing may be impaired by wound and host factors and thus more advanced therapies are required for a fast and satisfactory outcome. Platelet-rich plasma (PRP), rich in growth factors and cytokines essential for tissue repair, could improve wound healing. The purpose of this experimental study was to evaluate the effect of locally injected autologous PRP on second intention healing of acute full-thickness skin defects in dogs.

View Article and Find Full Text PDF

Respiratory neoplasia is rarely reported in avian species. A 17-yr-old Quaker parrot (Myiopsitta monachus) was admitted with a 2-wk history of anorexia, depression, and respiratory distress. Clinical examination revealed a large, firm mass in the left pectoral muscle.

View Article and Find Full Text PDF
Article Synopsis
  • An outbreak of goiter in a flock of budgerigars in California caused significant illness and death, affecting 45 out of 400 birds, with 15 fatalities over 2-3 months.
  • The diet lacked essential minerals and included broccoli, which binds iodine, contributing to the health issues; examinations revealed severely enlarged thyroid glands in affected birds.
  • Iodine supplementation and removal of broccoli improved the situation, suggesting dietary modifications could prevent similar outbreaks in the future.
View Article and Find Full Text PDF

Clostridium septicum is the causative agent of histotoxic infections, including malignant edema and braxy (necrotizing abomasitis) in several animal species. The carcass of a 2-year-old, female New Zealand white rabbit with a history of acute depression and obtundation followed by death was received at the California Animal Health and Food Safety Laboratory System (San Bernardino, California) for necropsy and diagnostic workup. No gross lesions were detected at necropsy.

View Article and Find Full Text PDF

Foot-and-mouth disease (FMD) is a highly contagious, debilitating, and globally significant viral disease typically affecting cloven-hoofed hosts. The diagnosis of FMD in bears in Vietnam is described. The current study describes a confirmed case of FMD in a bear species, and the clinical signs compatible with FMD in a Malayan sun bear.

View Article and Find Full Text PDF

Objectives: Distal flap necrosis remains a major complication in subdermal plexus (random) skin flaps. Platelet-rich plasma (PRP) has been shown to improve the survival of ischemic random skin flaps in rats. The objective of this study was to evaluate the effect of locally injected autologous PRP on the survival of long (5:1 length-to-width ratio) subdermal plexus skin flaps in dogs.

View Article and Find Full Text PDF

This study was conducted to determine the prevalence and characteristics of Johne's disease (JD) lesions in Ugandan cattle slaughtered at two of the main abattoirs in Kampala. Ileocaecal junction and the associated lymph nodes of 1,022 cattle were examined for gross and microscopic lesions, followed by Ziehl Neelsen staining of the tissues bearing lesions. Confirmation of Mycobacterium avium subsp.

View Article and Find Full Text PDF

Background: The occurrence of paratuberculosis in Ugandan cattle has recently been reported but there is no information on the strains of Mycobacterium avium subspecies paratuberculosis (MAP) responsible for the disease. The aim of this study was to isolate and characterise MAP from seropositive cattle and paratuberculosis lesions in tissues obtained from slaughtered cattle in Uganda.

Results: Twenty one isolates of MAP were differentiated into 11 genotype profiles using seven genotyping loci consisting of Insertion Sequence 1311(IS1311), Mycobacterial interspersed repeat units (MIRU) (loci 2, 3), Variable number tandem repeats (VNTR) locus 32 and Short sequence repeats (SSR) (loci 1, 2 and 8).

View Article and Find Full Text PDF

Illthrift was observed in 20/60 lambs aged 40-45 days in a dairy sheep flock in Greece. Cryptosporidiosis had been diagnosed and successfully treated with halofuginone lactate a month earlier. Parasitological examinations were negative for endoparasites while hematology and biochemistry were unremarkable.

View Article and Find Full Text PDF

The enantiomeric naphthoquinones alkannins and shikonins (A/S) have been established as potent wound healing agents. The purpose of this study was to evaluate the effectiveness of an A/S based ointment for humans on second intention wound healing in the dog, as compared to wound flushing with Lactated Ringer's solution (LRS). Ten mixed breed dogs, aged 2 to 5 y, were used.

View Article and Find Full Text PDF

A seven-year-old entire female, mixed-breed dog, weighing 19 kg, was presented with a 2-day history of abdominal distension, reduced appetite and general dullness. Abdominal palpation revealed a large mass. Radiography showed a large mass occupying the left mid-abdominal area and convoluted loops of tubular fluid opacity occupying the right mid-abdominal area.

View Article and Find Full Text PDF

The Mediterranean monk seal (Monachus monachus) is one of the world's most endangered marine mammals. The largest population is located mainly throughout the Aegean and Ionian islands and along the coastline of southern continental Greece. We report the findings of a necropsy and discuss their potential importance to the conservation of the species.

View Article and Find Full Text PDF

Intestinal obstruction resulting from an intramural foreign body-associated pyogranuloma was diagnosed in four dogs. Vomiting and weight loss were the main clinical signs. On physical examination, a mass in the abdomen was detected in three dogs.

View Article and Find Full Text PDF