Publications by authors named "Louis G Jensen"
Article Synopsis
- Photoactivated localization microscopy (PALM) generates localization coordinates using photoactivatable fluorescent proteins, but suffers from issues like multiple blinking and localization errors, leading to false clustering in data.
- A new 'model-based correction' (MBC) workflow enhances accuracy by estimating blinking dynamics and refining clustering, yielding more precise localization coordinates that outperform current methods.
- The corrected data enables reliable tests for spatial randomness and quantitative analysis of fluorophore clusters, validated with simulated and experimental data, revealing clustering of an adapter protein at the T cell immunological synapse.
Regulated exocytosis: renal aquaporin-2 3D vesicular network organization and association with F-actin.
Am J Physiol Cell Physiol
December 2021
Regulated vesicle exocytosis is a key response to extracellular stimuli in diverse physiological processes, including hormone regulated short-term urine concentration. In the renal collecting duct, the water channel aquaporin-2 (AQP2) localizes to the apical plasma membrane as well as to small, subapical vesicles. In response to stimulation with the antidiuretic hormone, arginine vasopressin, aquaporin-2-containing vesicles fuse with the plasma membrane, which increases collecting duct water reabsorption and thus, urine concentration.
View Article and Find Full Text PDFArtificial indoor lighting can disturb sleep and increase depressive symptoms; both common complaints in psychiatric inpatients. In this trial we aimed to improve sleep in psychiatric inpatients using a circadian lighting environment. Investigator-blinded parallel-group randomised controlled effectiveness trial in an inpatient psychiatric ward with adjustable lighting.
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