Benzalkonium chloride in an orthodontic adhesive: Its effect on rat enamel demineralization using color-based image analysis.

Introduction: The aim of this study was to determine the effect of an orthodontic bonding adhesive containing benzalkonium chloride (BAC) on enamel demineralization.

Methods: Eighteen female Sprague-Dawley rats, aged 8 to 10 weeks, were inoculated with Streptococcus sobrinus for 5 days. The animals were randomly divided into the control, non-BAC, and BAC groups.