Development of a Cu-labeled CD4 T cell targeting PET tracer: evaluation of CD4 specificity and its potential use in collagen-induced arthritis.

Background: CD4 T cells are central inflammatory mediators in the pathogenesis of autoimmune rheumatoid arthritis (RA), as they are one of the dominating cell types in synovial inflammation. Molecular imaging of CD4 T cells has potential role for early detection and monitoring of RA. Here, we developed a new radiotracer for in vivo immunoPET imaging of murine CD4 T cells and tested it in the collagen-induced arthritis (CIA) mouse model of human RA.

Noninvasive Molecular Imaging of the Enhanced Permeability and Retention Effect by Cu-Liposomes: In vivo Correlations with Ga-RGD, Fluid Pressure, Diffusivity and F-FDG.

Background: The accumulation of liposome encapsulated chemotherapy in solid cancers is dependent on the presence of the enhanced permeability and retention (EPR) effect. Positron emission tomography (PET) imaging with a liposome encapsulated radioisotope, such as liposome encapsulated Cu-64 (Cu-liposome) may help to identify tumors with high liposome accumulation, and thereby stratify patients based on expected benefit from liposomal chemotherapy. However, intravenous administration of liposomes without a cytotoxic content is complicated by the accelerated blood clearance (ABC) phenomenon for succeeding therapeutic liposome dosing.

T-cells and macrophages peak weeks after experimental stroke: Spatial and temporal characteristics.

The activities of the central and peripheral immune systems impact neurological outcome after ischemic stroke. However, studies investigating the temporal profile of leukocyte infiltration, especially T-cell recruitment, are sparse. Our aim was to investigate leukocyte infiltration at different time points after experimental stroke in mice.