Novel synthetic co-culture of and using CO and in situ generated H for the production of caproic acid via lactic acid.

is known to produce mainly acetate from CO and H, but the production of higher value chemicals is desired for the bioeconomy. Using chain-elongating bacteria, synthetic co-cultures have the potential to produce longer-chained products such as caproic acid. In this study, we present first results for a successful autotrophic co-cultivation of mutants and a wild-type strain in a stirred-tank bioreactor for the production of caproic acid from CO and H via the intermediate lactic acid.

Regulation of pyruvate dehydrogenase complex related to lactate switch in CHO cells.

The metabolism of Chinese hamster ovary (CHO) cell lines is typically characterized by high rates of aerobic glycolysis with increased lactate formation, known as the "Warburg" effect. Although this metabolic state can switch to lactate consumption, the involved regulations of the central metabolism have only been partially studied so far. An important reaction transferring the lactate precursor, pyruvate, into the tricarboxylic acid cycle is the decarboxylation reaction catalyzed by the pyruvate dehydrogenase enzyme complex (PDC).

Cover Feature: Regulation of pyruvate dehydrogenase complex related to lactate switch in CHO cells.

DOI: 10.1002/elsc.202000037 The cover feature visualizes our recent article about the investigation of the regulation of the Pyruvate Dehydrogenase Complex (PDC) during the lactate switch in batch cultures of Chinese Hamster Ovary cells.