Post-prostatic massage fluid/urine as an alternative to semen for studying male genitourinary HIV-1 shedding.

Objectives: Genitourinary tract samples are required to investigate male HIV-1 infectivity. Because semen collection is often impractical, the acceptability, feasibility and validity of post-prostatic massage fluid/urine (post-PMF/U) was evaluated for studying male genitourinary HIV-1 shedding.

Methods: HIV-1-seropositive men were evaluated after 48 h of sexual abstinence.