Sensitive bioluminescence imaging of cryptococcosis in improves antifungal screening under conditions.

is an environmental yeast that primarily affects immunocompromised individuals, causing respiratory infections and life-threatening meningoencephalitis. Treatment is complicated by limited antifungal options, with concerns such as adverse effects, dose-limiting toxicity, blood-brain barrier permeability, and resistance development, emphasizing the critical need to optimize and expand current treatment options against invasive cryptococcosis. larvae have been introduced as an ethical intermediate for testing, bridging the gap between antifungal screening and mouse studies.