Development of microwave assisted-UV digestion using diluted reagents for the determination of total nitrogen in cereals by ion chromatography.

The objective of this work is to develop a microwave assisted-ultraviolet (MW-UV) digestion in the presence of dilute HCl and HO followed by ion chromatography (IC) measurements for the determination of total nitrogen in cereals. This approach (MW-UV-IC) requires lesser time and does not need environmentally hazardous materials as used in Kjeldhal method. Further, the developed method requires only microliter quantities of dilute HCl and few milliliters of HO for the matrix digestion and simultaneous conversion of nitrogen to its ionic species for the subsequent analysis by IC.

Intrinsic peroxidase-like activity of 4-amino hippuric acid reduced/stabilized gold nanoparticles and its application in the selective determination of mercury and iron in ground water.

Herein, we report a method for the synthesis of 4-aminohippuric acid (4-AHA) reduced/stabilized gold nanoparticles and their peroxidase mimicking properties for the colorimetric detection of Fe and Hg. The synthesis of nanoparticles was evidenced by appearance of bright red color and an absorption peak at 518 nm. Transmission electron microscopic (TEM) characterization revealed the nanoparticles to be spherical with average size of about 5.

Bacteriogenic synthesis of selenium nanoparticles by ATCC 35218 and its structural characterisation.

A biosynthetic method for the production of selenium nanoparticles under ambient temperature and pressure from sodium selenite was developed using Gram-negative bacterial strain ATCC 35218. Bacteriogenic nanoparticles were methodologically characterized employing UV-vis, XRD, Raman spectroscopy, SEM, TEM, DLS and FTIR techniques. Generation of nanoparticles was visualized from the appearance of red colour in the selenite supplemented culture medium and broad absorption bands in the UV-vis.

Biomimetic synthesis of selenium nanoparticles by Pseudomonas aeruginosa ATCC 27853: An approach for conversion of selenite.

A facile and green method for the reduction of selenite was developed using a Gram-negative bacterial strain Pseudomonas aeruginosa, under aerobic conditions. During the process of bacterial conversion, the elemental selenium nanoparticles were produced. These nanoparticles were systematically characterized using various analytical techniques including UV-visible spectroscopy, XRD, Raman spectroscopy, SEM, DLS, TEM and FTIR spectroscopy techniques.

Facile synthesis of palladium nanocatalyst using gum kondagogu (Cochlospermum gossypium): a natural biopolymer.

Palladium nanoparticles (Pd NPs) were synthesised by using gum kondagogu (GK), a non-toxic ecofriendly biopolymer. GK acted as both reducing and stabilising agent for the synthesis of Pd NPs. Various reaction parameters, such as concentration of gum, Pd chloride and reaction pH were standardised for the stable synthesis of GK reduced stabilised Pd NPs (GK-Pd NPs).

Gum kondagogu reduced/stabilized silver nanoparticles as direct colorimetric sensor for the sensitive detection of Hg²⁺ in aqueous system.

A highly sensitive and selective method is reported for the colorimetric detection of Hg(2+) in aqueous system by using label free silver nanoparticles (Ag NPs). Ag NPs used in this method were synthesized by gum kondagogu (GK) which acted as both reducing and stabilizing agent. The average size of the GK-Ag NPs was found to be 5.

Enhancement of antibacterial activity of capped silver nanoparticles in combination with antibiotics, on model gram-negative and gram-positive bacteria.

The nanoparticles used in this study were prepared from AgNO3 using NaBH4 in the presence of capping agents such as citrate, sodium dodecyl sulfate, and polyvinylpyrrolidone. The formed nanoparticles were characterized with UV-Vis, TEM, and XRD. The generation of silver nanoparticles was confirmed from the appearance of yellow colour and an absorption maximum between 399 and 404 nm.

Synthesis and characterization of bovine serum albumin-copper nanocomposites for antibacterial applications.

A method for the synthesis of bovine serum albumin (BSA) and copper (Cu(0)) nanocomposites is described. The synthesis is achieved by adding [100mM] hydrazine hydrate ((N2H4·H2O) to [10mM] copper sulfate (CuSO4·5H2O) solution in the presence of 0.02% bovine serum albumin at pH-10.

Highly stable, protein capped gold nanoparticles as effective drug delivery vehicles for amino-glycosidic antibiotics.

A method for the production of highly stable gold nanoparticles (Au NP) was optimized using sodium borohydride as reducing agent and bovine serum albumin as capping agent. The synthesized nanoparticles were characterized using UV-visible spectroscopy, transmission electron microscopy, X-ray diffraction (XRD) and dynamic light scattering techniques. The formation of gold nanoparticles was confirmed from the appearance of pink colour and an absorption maximum at 532 nm.

Protection against radiation-induced oxidative damage by an ethanolic extract of Nigella sativa L.

Purpose: An ethanolic extract of Nigella sativa L. (EE-NS) was investigated for its antioxidant properties and radioprotective effects against gamma-radiation-induced oxidative damage.

Materials And Methods: The radical scavenging activity of the extract was measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH), deoxyribose degradation and plasmid relaxation assays in a cell-free system.