Characterization of an in vitro model of intestinal cells as a test-bed for mCIPO therapies.

Chronic Intestinal Pseudo-Obstruction in children (CIPO) comprises a group of ultra-rare and debilitating diseases characterized by severe motility defects affecting the entire digestive tract. With limited effective therapies, particularly for myopathic CIPO, we aim at characterizing the behavior of an intestinal smooth muscle cell line undergoing a set of chemical and physical stimulations, to assess their effect. To this end, we adopted healthy Human Intestinal Smooth Muscle Cells, plated on standard coverslips and, for the first time, Micro Electrode Arrays (MEAs).