Publications by authors named "Lorenzo Bracaglia"
Article Synopsis
- Nuclear magnetic resonance (NMR) spectroscopy is a technique used to analyze biomolecules like proteins and nucleic acids with high precision, especially with the introduction of powerful 28.2 T magnets since 2019.
- This new ultra-high-field NMR allows researchers to study complex biomolecular structures, including flexible proteins known as intrinsically disordered proteins (IDPs) and regions (IDRs), which traditionally faced challenges due to crowded NMR spectra.
- The protocol offers methods to optimize high-field NMR experiments for IDPs/IDRs, including tailored experiments and strategies based on protein relaxation analysis, applicable to different molecular weights and complexities, as well as to lower field strengths.
Cell migration requires a complex array of molecular events to promote protrusion at the front of motile cells. The scaffold protein LL5β interacts with the scaffold ERC1, and recruits it at plasma membrane-associated platforms that form at the front of migrating tumor cells. LL5 and ERC1 proteins support protrusion during migration as shown by the finding that depletion of either endogenous protein impairs tumor cell motility and invasion.
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