Publications by authors named "Lorenzo Barolo"
Article Synopsis
- Chlamydomonas reinhardtii is being explored as a system to produce recombinant proteins, but it struggles with consistent gene expression due to issues like gene silencing and low transgene activity.
- A study engineered this alga to express a fluorescent protein and analyzed the changes in its intracellular proteome, revealing over 400 proteins with altered abundance and various impacted biological pathways.
- The findings shed light on how transformation affects gene expression, suggesting potential targets for future genetic modifications to enhance C. reinhardtii's effectiveness as a biofactory for industrial uses.
Article Synopsis
- Tauopathies like Alzheimer's and Frontotemporal Dementia are severe brain disorders that cause cognitive decline, and accurate diagnosis is essential for developing effective treatments.
- The study presents a new approach using humanized ferritin nanocages to deliver a tau-specific fluorophore (BT1) into human retinal cells, which helps detect neurofibrillary tangles associated with these conditions.
- This innovative method showcases the potential of nanotechnology in improving the early detection and diagnosis of tauopathies, marking a significant advancement in nanobiotechnology and neurodegenerative disease management.
Chronic lymphocytic leukemia (CLL) is a widespread type of leukemia that predominantly targets B lymphocytes, undermining the balance between cell proliferation and apoptosis. In healthy B cells, miR-15/16, a tandem of microRNAs, functions as a tumor suppressor, curbing the expression of the antiapoptotic B cell lymphoma 2 protein (Bcl-2). Conversely, in CLL patients, a recurring deletion on chromosome 13q14, home to the miR15-a and miR16-1 genes, results in Bcl-2 overexpression, thereby fostering the onset of the pathology.
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- The green microalga Chlamydomonas reinhardtii, specifically the mutant strain UVM4, is a promising option for producing secreted recombinant proteins (RPs) but faces challenges in purification.
- Recent analysis shows that UVM4 has a unique extracellular proteome with an abundance of cell wall glycoproteins, particularly pherophorins, which contribute to difficult RP isolation.
- Understanding the nature of these extracellular aggregates will help develop better strategies for purifying RPs and potentially increasing biopharmaceutical yields.
Microalgae exhibit great potential for recombinant therapeutic protein production, due to lower production costs, immunity to human pathogens, and advanced genetic toolkits. However, a fundamental aspect to consider for recombinant biopharmaceutical production is the presence of correct post-translational modifications. Multiple recent studies focusing on glycosylation in microalgae have revealed unique species-specific patterns absent in humans.
View Article and Find Full Text PDFUnderstanding the relationship between physical exercise, reactive oxygen species, and skeletal muscle modification is important in order to better identify the benefits or the damages that appropriate or inappropriate exercise can induce. Heart and skeletal muscles have a high density of mitochondria with robust energetic demands, and mitochondria plasticity has an important role in both the cardiovascular system and skeletal muscle responses. The aim of this study was to investigate the influence of regular physical activity on the oxidation profiles of mitochondrial proteins from heart and tibialis anterior muscles.
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