Photosensitized isomerization of resveratrol: Evaluation of energy and electron transfer pathways.

Resveratrol (3,5,4'-trihydroxystilbene, RSV) is a natural stilbene synthetized as trans-isomer in plants exposed to oxidative stress. In order to understand the mechanism involved during photosensitized degradation of trans-resveratrol, steady-state and time-resolved experiments were performed and compared with quantum-chemical calculations using density functional theory (DFT). Pterin (Ptr), a well-known photosensitizer, under UV-A radiation induces the oxidation of several biomolecules mainly through electron-transfer mechanisms.

Unanticipated difficult airway after prolonged use of a laryngeal mask airway.

The laryngeal mask airway (LMA) is recognized as a safe alternative to endotracheal intubation for short-term airway maintenance. In this case report we present the case of a term neonate with upper airway obstruction which was managed with a deflated LMA for 7 consecutive days. Despite previous reports of extended LMA use in neonates without complications, this patient experienced significant pharyngeal mucosal injury and edema, leading to difficulty with subsequent intubation attempts.

Ivermectin bioaccumulation and transfer through developmental stages in Culex pipiens (Diptera: Culicidae).

Ivermectin (IVM), one of the most widely used antiparasitics in livestock, could enters into the aquatic environment because the treated animal metabolizes only a small percentage of what is administered and the rest is eliminated through the feces, largely as a parent drug, imposing a risk to aquatic organisms. The aims of this study were to (1) assess the effect of IVM spiked in cattle dung on the survival and emergence of Culex pipiens (Diptera: Culicidae), and to (2) evaluate the accumulation of this drug in the different developmental stages of this taxon. Larvae were exposed to two IVM concentrations (T1: 1000 ng g and T2: 500 ng g) for 9 days.

Proteomic analysis of the mandibular glands from the Chinese crocodile lizard, Shinisaurus crocodilurus - Another venomous lizard?

Based on its phylogenetic relationship to monitor lizards (Varanidae), Gila monsters (Heloderma spp.), and the earless monitor Lanthanotus borneesis, the Chinese crocodile lizard, Shinisaurus crocodilurus, has been assigned to the Toxicofera clade, which comprises venomous reptiles. However, no data about composition and biological activities of its oral secretion have been reported.

Treatment of impacted or retained second molars with the miniscrew-supported pole technique: a prospective follow-up study.

Background: Eruption disturbances of permanent molars are uncommon; however, it is important to treat them as soon as they are diagnosed. The main objective was to analyze the effectiveness of the "miniscrew-supported pole technique," a surgically assisted orthodontic procedure to force the eruption of impacted/retained second molars (M2s) when there are indicators of complex molar inclusion. An observational prospective study was carried out during a 2-year period.

Avoiding One-Electron Oxidation of Biomolecules by 3,4-Dihydroxy-L-Phenylalanine (DOPA).

It has been proposed that 3,4-dihydroxy-L-phenylalanine (DOPA) has antioxidant properties, and thus, the objective of this work was to evaluate the effect of adding DOPA during the photosensitized oxidation of tyrosine (Tyr), tryptophan (Trp), histidine (His), 2'-deoxyguanosine 5'-monophosphate (dGMP) and 2'-deoxyadenosine 5'-monophosphate (dAMP). It was observed that, upon pterin-photosensitized degradation of a given biomolecule in acidic aqueous solutions, the rate of the biomolecule consumption decreases due to the presence of DOPA. Although DOPA deactivates the excited states of pterin (Ptr), biomolecules do as well, being the bimolecular quenching constants in the diffusional control limit, indicating that DOPA antioxidant mechanism is not a simple deactivation of Ptr excited states.

Negative predictive value of procalcitonin to rule out bacterial respiratory co-infection in critical covid-19 patients.

Background: Procalcitonin (PCT) and C-Reactive Protein (CRP) are useful biomarkers to differentiate bacterial from viral or fungal infections, although the association between them and co-infection or mortality in COVID-19 remains unclear.

Methods: The study represents a retrospective cohort study of patients admitted for COVID-19 pneumonia to 84 ICUs from ten countries between (March 2020-January 2021). Primary outcome was to determine whether PCT or CRP at admission could predict community-acquired bacterial respiratory co-infection (BC) and its added clinical value by determining the best discriminating cut-off values.

Photosensitized Dimerization of Tyrosine: The Oxygen Paradox.

In electron-transfer initiated photosensitization processes, molecular oxygen (O ) is not involved in the first bimolecular event, but almost always participates in subsequent steps giving rise to oxygenated products. An exception to this general behavior is the photosensitized dimerization of tyrosine (Tyr), where O does not participate as a reactant in any step of the pathway yielding Tyr dimers (Tyr ). In the pterin (Ptr) photosensitized oxidation of Tyr, O does not directly participate in the formation of Tyr and quenches the triplet excited state of Ptr, the reactive species that initiates the process.

Description of an Ultrasound-Guided Transverse Approach to the Transversus Thoracis Plane Block and Evaluation of Injectate Spread in Canine Cadavers.

Transversus thoracis plane (TTP) block has demonstrated to produce analgesia in humans undergoing median sternotomy. The objectives of the study were to describe an ultrasound-guided transverse approach to the transversus thoracis plane (t-TTP) and to evaluate the spread of two injectable volumes in canine cadavers. Two cadavers were used to describe relevant gross anatomy of the ventral thoracic area and sonoanatomy between the fifth and sixth costal cartilages.

Pterin-photosensitization of thymine under anaerobic conditions in the presence of guanine.

Pterin (Ptr) is a model photosensitizer that acts mainly through type I mechanism and is able to photoinduce the one-electron oxidation of purine and pyrimidine nucleobases. However, under anaerobic conditions Ptr reacts with thymine (T) to form photoadducts (Ptr-T) but does not lead to the photodegradation of guanine (G), which is the nucleobase with the lowest ionization potential. Accordingly, G is thermodynamically able to reduce the radicals of the other nucleobases and has been described in this sense as the "hole sink" of the DNA double helix.

Orthodontic management of severe iatrogenic biprotrusion and resorptions with miniscrews and corticotomies.

Background: This case report describes a patient with severe iatrogenic dental biprotrusion who visited for a second assessment. The patient presented first premolar maxillary extractions, resorbed maxillary incisors and dehiscences in the anterior buccal and palatal cortical bone diagnosed with cone-beam computed tomography (CBCT).

Material And Methods: At the beginning of treatment, fixed appliances were bonded on all teeth except on the upper incisors to prevent further root resorption.

Oxidation of tyrosine: Antioxidant mechanism of l-DOPA disclosed.

Tyrosine is an amino acid related to crucial physiological events and its oxidation, that produce beneficial or detrimental effects on biological systems, has been extensively studied. Degradation of tyrosine often begins with the loss of an electron in an electron transfer reaction in the presence of a suitable electron acceptor. The reaction is facilitated by excited states of the acceptor in photosensitized processes.

Miniscrew-supported pole technique: Surgical-orthodontic approach for impacted or retained second molars in adolescents.

Background: Several treatment options have been proposed for the treatment of eruption disturbances of permanent molars. Despite being an infrequent condition, these disturbances should be solved as they can lead to important complications and play a relevant role in completing the occlusion.

Findings: The presented cases involved maxillary and mandibular included second molars (M2s) respectively.

Orthodontic management of a complete and an incomplete maxillary canine-first premolar transposition.

Maxillary canine and first premolar transposition is a complicated dental anomaly to treat, especially if the clinician's goal is to orthodontically move the canine into its normal position. Early diagnosis with cone-beam computed tomography simplifies the treatment of this pathology. This case report describes a patient with bilateral transposition, one complete and the other incomplete, involving the maxillary canine and the first premolar (Mx.

Age- and Sex-Related Modifications of Hematology in Spanish Purebred Horse.

The purpose of this study was to test the hypothesis that age and sex significantly modulate the changes in hematological parameters of physiologically healthy Spanish Purebred horse, establishing the reference values. Blood samples were obtained on a total of 159 horses (n = 77 males and n = 82 females), classified in four age groups: group A (1-2 years; n = 39), group B (>2-3 years; n = 38), group C (>3-4 years; n = 41), and group D (>4-7 years; n = 41). By means ADVIA 2020i, red blood cell (RBC), hemoglobin concentration (HB), packed cell volume, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration, platelets (PLTs), as well as total and differential white blood cells (WBCs) were determined.

Type I Photosensitized Oxidation of Methionine.

Methionine (Met) is an essential sulfur-containing amino acid, sensitive to oxidation. The oxidation of Met can occur by numerous pathways, including enzymatic modifications and oxidative stress, being able to cause relevant alterations in protein functionality. Under UV radiation, Met may be oxidized by direct absorption (below 250 nm) or by photosensitized reactions.

Synergistic effect of carboxypterin and methylene blue applied to antimicrobial photodynamic therapy against mature biofilm of .

The control of multidrug-resistant (MDR) bacteria is a growing public health problem, and new strategies are urgently needed for the control of the infections caused by these microorganisms. Notoriously, some MDR microorganisms generate complex structures or biofilms, which adhere to surfaces and confer extraordinary resistance properties that are fundamental challenges to control infections. One of the promising strategies for the control of MDR bacteria is antimicrobial photodynamic therapy (aPDT), which takes advantage of suitable photosensitizers (PS), oxygen and radiation to eradicate microorganisms by the generation of highly reactive species, including reactive oxygen species (ROS) that cause cytotoxic damage and cell death.

Quad-helix compression to decompensate molar inclination prior to skeletal expansion.

Objectives: To demonstrate that patients without posterior crossbite (PCB) but with maxillary transverse deficiency, not previously observed due to an increased curve of Wilson, can benefit from the same palatal expansion as patients with PCB, after correction of the buccal inclination.

Materials And Methods: A total of 41 patients presenting a maxillary skeletal transverse deficiency were treated: 26 without PCB and 15 with PCB. In the non-PCB group, quad-helix compression was followed by a Hyrax expander (QH+HY), whereas the PCB group only underwent Hyrax expander treatment (HY).

[Mutational status of BRAF and histopathological characteristics in cutaneous malignant melanoma].

Malignant melanoma (MM) is the more aggressive form of skin cancer with a mortality rate in Argentina 1997-2001 = 1.1/100 000 in men and 0.6 in women.

Surgical-orthodontic approach for facial rejuvenation based on a reverse facelift.

Background: Orthodontic treatment combined with a maxillomandibular advancement (MMA) can be an effective option for patients who need not only corrected occlusion but also facial rejuvenation. In this case series, two patients underwent orthodontic treatment and bimaxillary orthognathic surgery involving MMA, one of them with a counterclockwise rotation of the occlusal plane (OP).

Findings: In both cases, the face was rejuvenated, a functional occlusion was established, and the posterior airway space (PAS) was widened.

Evidence of the effectiveness of Resveratrol in the prevention of guanine one-electron oxidation: possible benefits in cancer prevention.

Over the past few years, the interest in Resveratrol (3,4',5,-trihydroxystilbene, RSV) has increased due to the evidence found of its antioxidant action that protects biomolecules and cells from oxidative damage. The interest has been further exacerbated by the natural presence of RSV in some fruits and derivatives, especially in red wine. In this paper we present evidence of RSV capacity in protecting a deoxynucleotide, an essential constituent of DNA, from one-electron oxidation.

Distribution of molecular subtypes of advanced lung adenocarcinoma and clinical outcomes in a center of Argentina.

The prevalence of relevant oncogenic drivers in lung adenocarcinoma varies in our region and data on clinical outcomes is scarce. The objective of the study was to describe the prevalence of KRAS, BRAF and EGFR mutations and ALK translocations in patients with advanced lung adenocarcinoma, and to depict the clinical outcome according to treatment strategies. Patients with adequate tumor biopsy sampling were included.

Management of Deeply Impacted Molars with the Miniscrew-Supported Pole Technique.

Impacted molars occur more often in the mandibular arch than in the maxillary arch, with an overall prevalence of .01-1.8%.