The Global Challenge of : Antimicrobial Resistance and Emerging Intervention Strategies.

Antimicrobial resistance (AMR) in species, particularly and , poses a significant public health threat. These bacteria, which are commonly found in livestock, poultry, companion animals, and wildlife, are the leading causes of foodborne illnesses, often transmitted through contaminated poultry. Extensive exposure to antibiotics in human and veterinary medicine creates selection pressure, driving resistance through mechanisms such as point mutations, horizontal gene transfer, and efflux pumps.

Three-Dimensional Printing/Bioprinting and Cellular Therapies for Regenerative Medicine: Current Advances.

The application of three-dimensional (3D) printing/bioprinting technologies and cell therapies has garnered significant attention due to their potential in the field of regenerative medicine. This paper aims to provide a comprehensive overview of 3D printing/bioprinting technology and cell therapies, highlighting their results in diverse medical applications, while also discussing the capabilities and limitations of their combined use. The synergistic combination of 3D printing and cellular therapies has been recognised as a promising and innovative approach, and it is expected that these technologies will progressively assume a crucial role in the treatment of various diseases and conditions in the foreseeable future.

Respiratory muscle training in people with cervical spinal cord injury - A systematic review.

Introduction: Spinal cord injury is a physiological disruption often caused by trauma, leading to severe physical and psychological effects, including irreversible impairment and disability. Cervical injuries, particularly between C1 and C8, are the most severe, potentially causing diaphragm paralysis and requiring mechanical ventilation. Reduced respiratory muscle strength not only affects respiratory function but also significantly impacts voice, speech, and communication, which are crucial for quality of life.

Stem Cell-Based Therapies for Glaucoma Treatment: A Review Bridging the Gap in Veterinary Patients.

Retinal diseases are characterized by progressive damage to retinal cells, leading to irreversible vision loss. Among these, glaucoma stands out as a multifactorial neurodegenerative disease involving elevated intraocular pressure, retinal ganglion cell apoptosis, and optic nerve damage, ultimately resulting in blindness in both humans and dogs. Stem cell-based therapies have emerged as a promising therapeutic option for such conditions due to their regenerative and neuroprotective potential.

Isolation, Expansion, and Characterization of Rat Hair Follicle Stem Cells and Their Secretome: Insights into Wound Healing Potential.

Stem cells are capable of self-renewal and differentiation into various specialized cells, making them a potential therapeutic option in regenerative medicine. This study establishes a comprehensive methodology for isolating, culturing, and characterizing rat hair follicle stem cells. Hair follicles were harvested from Sprague-Dawley rats and subjected to two different isolation techniques.

Characterization of risk factors for early ambulation in paraplegic dogs with absent pain perception undergoing decompressive surgery for thoracolumbar intervertebral disk extrusions.

Background: Current literature warrants surgical decompression in paraplegic dogs with absent pain perception (APP), but the rate of ambulatory dogs with APP following thoracolumbar (TL) IVDE surgery in a clinical setting remains unknown. Furthermore, the outcome of paraplegic APP French Bulldogs (FBs) is anecdotally considered poor. The aims of this study were threefold within a large population of TL-IVDE paraplegic dogs with APP undergoing decompressive surgery: (1) to characterize early spontaneous pelvic limb movement and ambulation following surgery; (2) to identify risk factors for the recovery of ambulation; and (3) to compare the outcome of FBs and Dachshunds presenting with APP.

Innovative Ink-Based 3D Hydrogel Bioprinted Formulations for Tissue Engineering Applications.

Three-dimensional (3D) models with improved biomimicry are essential to reduce animal experimentation and drive innovation in tissue engineering. In this study, we investigate the use of alginate-based materials as polymeric inks for 3D bioprinting of osteogenic models using human bone marrow stem/stromal cells (hBMSCs). A composite bioink incorporating alginate, nano-hydroxyapatite (nHA), type I collagen (Col) and hBMSCs was developed and for extrusion-based printing.

Direct-detection of glyphosate in drinking water a scalable and low-cost laser-induced graphene sensor.

The use of pesticides has significantly increased and proliferated following the technological advancements established by the green revolution, aimed at boosting agricultural productivity. The extensive use of man-made chemicals as fertilizer and pesticides has consequently led to large-scale application, which has led to a number of environmental and human health problems. This study has helped to develop a laser-induced graphene (LIG) sensor for the detection of the most widely used herbicide in the world, glyphosate.

Enhancing corneal ectasia susceptibility detection: analysis of a new algorithm (BAD-D v4).

Accurate detection of post-refractive ectasia susceptibility is essential during preoperative evaluation for laser vision correction (LVC) due to the risk of progressive corneal ectasia and vision decline post-surgery. Despite improved screening and a reduced incidence from 0.66 to 0.

MPO interacts with hRSV particles, contributing to the virucidal effects of NETs against clinical and laboratory hRSV isolates.

Human Respiratory syncytial virus (hRSV) mainly affects immunosuppressed patients requiring hospitalization. No specific treatment is financially accessible, and available vaccines do not cover all risk groups. During hRSV infection, there is a robust neutrophilic influx into the airways.

Corneal Layer Thickness Mapping With Optical Coherence Tomography in Different Stages Reveals Pathologic Processes of Keratoconus.

Purpose: The aim of this study was to characterize the corneal layer thickness profile in different stages of keratoconus (KC).

Methods: Patients with clinical or subclinical KC without a history of contact lens use were retrospectively enrolled. We evaluated 164 eyes, including 69 clinical KC, 32 topographic KC (no clinical signs), 22 very asymmetric ectasia with normal topography and no clinical signs of ectasia (VAE-NT), and 41 control eyes.

Unilateral molar Incisor hypomineralization influences the chewing side? an observational study in children.

Objective: The aim of this observational study was to compare the chewing patterns of children with unilateral Molar Incisor Hypomineralization (MIH) to those of children without MIH and to assess how the MIH severity influences the risk of chewing deviation. The hypothesis was that children with unilateral MIH would prefer to chew on the side opposite the defect.

Methods: A total of 121 children were included in this investigation and evaluated for their preferred chewing side (PCS).

Acute toxicity of trypsin inhibitor from tamarind seeds in embryo and adult zebrafish ().

The trypsin inhibitor isolated from tamarind seeds (TTI) is being investigated for potential applications in the treatment of noncommunicable diseases (NCD), such as hypertension, obesity, and diabetes. This study aimed to assess TTI embryotoxicity and acute toxicity in adult zebrafish (). TTI was extracted and isolated from tamarind seeds.

Spatial, transcriptomic, and epigenomic analyses link dorsal horn neurons to chronic pain genetic predisposition.

Key mechanisms underlying chronic pain occur within the dorsal horn. Genome-wide association studies (GWASs) have identified genetic variants predisposed to chronic pain. However, most of these variants lie within regulatory non-coding regions that have not been linked to spinal cord biology.

Antimicrobial Resistance in Diverse Pathotypes from Nigeria.

is a gram-negative commensal bacterium living in human and animal intestines. Its pathogenic strains lead to high morbidity and mortality, which can adversely affect people by causing urinary tract infections, food poisoning, septic shock, or meningitis. Humans can contract by eating contaminated food-such as raw or undercooked raw milk, meat products, and fresh produce sold in open markets-as well as by coming into contact with contaminated settings like wastewater, municipal water, soil, and faeces.

Observation of the decay.

Using proton-proton collision data corresponding to an integrated luminosity of collected by the CMS experiment at , the decay is observed for the first time, with a statistical significance exceeding 5 standard deviations. The relative branching fraction, with respect to the decay, is measured to be , where the first uncertainty is statistical, the second is systematic, and the third is related to the uncertainties in and .

Investigating tributyltin's toxic effects: Intestinal barrier and neuroenteric disruption in rat's jejunum.

The expansion of economic activities in coastal areas has significantly increased chemical contamination, leading to major environmental challenges. Contaminants enter the human body through the food chain, particularly via seafood and water consumption, triggering biomagnification and bioaccumulation processes. The gastrointestinal tract (GIT) acts as a selective barrier, protecting against chemical pollutants and maintaining homeostasis through a complex network of cells and immune responses.

Antibiotic Resistance in Gram-Negative Bacteria: The Threat from the Pink Corner.

Antibiotic resistance in Gram-negative bacteria is a formidable challenge in modern medicine [...

Genetic landscape of ESBL producing international clone ST410 of from pediatric infections in Shenzhen, China.

Background: The emergence of ESBLs producing cephalosporin-resistant isolates poses a threat to public health. This study aims to decipher the genetic landscape and gain insights into ESBL-producing strains belonging to the high-risk clone ST410 from pediatric patients.

Methods: 29 ST410 isolates were collected from young children and subjected to antimicrobial susceptibility testing, Whole-genome sequencing (WGS), serotype analysis, MLST, ESBL genes, virulence genes, and plasmid profiling.

Characterisation of cells markers associated with IKZF1 in BCP-ALL.

The presence of IKZF1 deletions has been associated with an increased relapse rate in B-cell precursor acute lymphoblastic leukaemia (BCP-ALL). There is a particular subset of IKZF1 cases called IKZF1 (defined by the co-occurrence of IKZF1 and deletions in CDKN2A/B, PAX5, or the PAR1 region, in the absence of ERG deletions), which is also associated with worse prognosis, but some recent studies have not found major differences between the IKZF1 and IKZF1 groups. Therefore, the IKZF1 group still needs further comprehension and our study aims to characterise the molecular heterogeneity and identify molecular markers exclusively associated with IKZF1.