Performance Evaluation of Molecular Detection of Enteroviruses: Results of 18 Years of Quality Control for Molecular Diagnostics (QCMD) External Quality Assessment Program, 2005-2022.

The gold standard for enterovirus (EV) detection is the polymerase chain reaction based on the detection of the 5' untranslated region of the virus. Correct detection of EV is crucial for patient and public health purposes. The performance of diagnostic and public health laboratories on molecular EV-detection was analyzed using data from the external quality assessment program distributed by Quality Control for Molecular Diagnostics (QCMD) between 2005 and 2022.

The role of cholinergic signaling in multi-sensory gamma stimulation induced perivascular clearance of amyloid.

Modulatory neurotransmitters exert powerful control over neurons and the brain vasculature. Gamma Entrainment Using Sensory Stimuli (GENUS) promotes amyloid clearance via increased perivascular cerebral spinal fluid (CSF) flux in mouse models of Alzheimer's Disease. Here we use whole-brain activity mapping to identify the cholinergic basal forebrain as a key region responding to GENUS.

Imagined otherness fuels blatant dehumanization of outgroups.

Dehumanization of others has been attributed to institutional processes that spread dehumanizing norms and narratives, as well as to individuals' denial of mind to others. We propose that blatant dehumanization also arises when people actively contemplate others' minds. We introduce the construct of imagined otherness-perceiving that a prototypical member of a social group construes an important facet of the social world in ways that diverge from the way most humans understand it-and argue that such attributions catalyze blatant dehumanization beyond the effects of general perceived difference and group identification.

Improved Wound Healing by Direct Cold Atmospheric Plasma Once or Twice a Week: A Randomized Controlled Trial on Chronic Venous Leg Ulcers.

This study compared the effect of two frequencies of direct cold atmospheric plasma (direct-CAP) treatment with standard of care (SOC) alone on healing of venous leg ulcers (VLUs). Open-label, randomized controlled trial (ClinicalTrials.gov NCT04922463) on chronic VLUs at two home care organizations in the Netherlands.

Feasibility of a Novel Geriatric Rehabilitation Program for People With COPD-induced Malnutrition and Muscle Wasting: A Qualitative Study.

Patients with COPD-induced malnutrition and muscle wasting are often frail. Consequently, traditional rehabilitation may be even counterproductive due to energy costs and there is a need for specialized rehabilitation programs, which are lacking for these patients. We developed such a program, which includes resistance training, following Nonlinear Periodized Exercise principles and physical energy management, in combination with a restriction of physical activities.

Correlated x-ray fluorescence and ptychographic nano-tomography on Rembrandt's reveals unknown lead "layer".

, one of the most famous masterpieces by Rembrandt, is the subject of a large research and conservation project. For the conservation treatment, it is of great importance to understand its current condition. Correlated nano-tomography using x-ray fluorescence and ptychography revealed a-so far unknown-lead-containing "layer", which likely acts as a protective impregnation layer applied on the canvas before the quartz-clay ground was applied.

Qualitative Assessment of a Human Trafficking Module Intervention in Pharmacy Education.

Human trafficking is a critical public health threat in the United States, yet education on human trafficking for pharmacy students has not been evaluated despite pharmacists being the most accessible health care professionals. To evaluate the most valuable aspects of an introductory module on human trafficking, pharmacy students participated in a human trafficking module, and their perception of human trafficking was evaluated after the module. Content on human trafficking was delivered through an asynchronous online presentation followed by a synchronous Zoom class discussion.

Experiences of Ambulatory Patients With Huntington's Disease With Case Management: A Qualitative Study.

Purpose/objectives: Huntington's disease (HD) requires high-quality care to reduce disruption of the patient system, prevent crisis situations, and prevent early admission in a nursing home. In the Netherlands, case management has been available for the last to 9 years for people with HD. However, there is a notable gap in understanding experiences and beliefs of HD patients regarding case managers' care, guidance, and support for quality of life.

iPSC-derived microglia carrying the TREM2 R47H/+ mutation are proinflammatory and promote synapse loss.

Genetic findings have highlighted key roles for microglia in the pathology of neurodegenerative conditions such as Alzheimer's disease (AD). A number of mutations in the microglial protein triggering receptor expressed on myeloid cells 2 (TREM2) have been associated with increased risk for developing AD, most notably the R47H/+ substitution. We employed gene editing and stem cell models to gain insight into the effects of the TREM2 R47H/+ mutation on human-induced pluripotent stem cell-derived microglia.

Changing roles of the corresponding author - Consequences for the accessibility of medical information.

It appears that ever more frequently the corresponding author of a multi-author manuscript is not what he/she was originally supposed to be: the most involved researcher with the best overview concerning the presented study. Numerous journals now use the term 'corresponding author', however, for the author who acts as a kind of secretary for the submitted manuscript, irrespective of his/her expertize in the subject. Another problem is that a significant number of universities give more scientific credits to the corresponding author than to his/her co-authors, which fairly commonly results in granting the corresponding authorship to the student or young scientist who needs scientific credits most urgently for his/her academic career.

Neuronal DNA double-strand breaks lead to genome structural variations and 3D genome disruption in neurodegeneration.

Persistent DNA double-strand breaks (DSBs) in neurons are an early pathological hallmark of neurodegenerative diseases including Alzheimer's disease (AD), with the potential to disrupt genome integrity. We used single-nucleus RNA-seq in human postmortem prefrontal cortex samples and found that excitatory neurons in AD were enriched for somatic mosaic gene fusions. Gene fusions were particularly enriched in excitatory neurons with DNA damage repair and senescence gene signatures.

Whole-genome screens reveal regulators of differentiation state and context-dependent migration in human neutrophils.

Neutrophils are the most abundant leukocyte in humans and provide a critical early line of defense as part of our innate immune system. We perform a comprehensive, genome-wide assessment of the molecular factors critical to proliferation, differentiation, and cell migration in a neutrophil-like cell line. Through the development of multiple migration screen strategies, we specifically probe directed (chemotaxis), undirected (chemokinesis), and 3D amoeboid cell migration in these fast-moving cells.

The effectiveness of the Dutch Meaningful Roles program in children: a study protocol for a cluster randomized controlled study.

Background: A positive, prosocial classroom climate is associated with improved social competence and academic achievement, as well as with decreased internalizing problems and antisocial behavior in children. It is expected that motivation to behave prosocially is needed to achieve a prosocial climate in the classroom, and that such motivation can be enhanced through three components of self-determination theory (SDT): competence, relatedness, and autonomy. The goal of this protocol is to describe the design of a study aiming to evaluate the effectiveness of a classroom-based program based on SDT components promoting a prosocial classroom climate.

Keeping medical science trustworthy: The threat by predatory journals.

Since most scientific journals tend to ask article processes costs from authors, a new category of journals has developed of which the business model is commonly exclusively based on financial contributions by authors. Such journals have become known as predatory journals. The financial contributions that they ask are not always lower than those asked by high-quality journals although they offer less: there is commonly no real review, texts are not edited, and there are commonly no printed editions.

Nursing home residents with Huntington's disease: Heterogeneity in characteristics and functioning.

Background: In Huntington's disease (HD), admission to a nursing home (NH) is required in advanced disease stages. To gain insight in care needs, more knowledge is needed on the functioning of this group.

Objective: Describing patient and disease characteristics, their functioning, and gender differences.

Impaired Awareness in People with Severe Alcohol-Related Cognitive Deficits Including Korskoff's Syndrome: A Network Analysis.

Background: Impaired awareness of one's own functioning is highly common in people with Korsakoff's syndrome (KS). However, it is currently unclear how awareness relates to impairments in daily functioning and quality of life (QoL).

Methods: We assessed how impaired awareness relates to cognitive, behavioral, physical, and social functioning and QoL by applying a network analysis.

Negative Associations in Word Embeddings Predict Anti-black Bias across Regions-but Only via Name Frequency.

The word embedding association test (WEAT) is an important method for measuring linguistic biases against social groups such as ethnic minorities in large text corpora. It does so by comparing the semantic relatedness of words prototypical of the groups (e.g.

Droughts are coming on faster.

Higher global temperatures are increasing the frequency of flash droughts.

A Cdk5-derived peptide inhibits Cdk5/p25 activity and improves neurodegenerative phenotypes.

Aberrant activity of cyclin-dependent kinase (Cdk5) has been implicated in various neurodegenerative diseases. This deleterious effect is mediated by pathological cleavage of the Cdk5 activator p35 into the truncated product p25, leading to prolonged Cdk5 activation and altered substrate specificity. Elevated p25 levels have been reported in humans and rodents with neurodegeneration, and the benefit of genetically blocking p25 production has been demonstrated previously in rodent and human neurodegenerative models.

The Effectiveness of School-Based Skills-Training Programs Reducing Performance or Social Anxiety: Two Randomized Controlled Trials.

Background: Given that high levels of stress during adolescence are associated with negative consequences, it is important that adolescents with psychological needs are supported at an early stage, for instance with interventions at school. However, knowledge about the potential of school-based programs targeting adolescents with psychological needs, aimed at reducing school or social stress, is lacking.

Objective: The current study aimed to investigate the effectiveness of two targeted school-based skills-training programs, addressing either skills to deal with performance anxiety or social skills.