The quaternary Gs3 and Gs7 allotypes of the rabbit: generation of the determinants by interspecies molecular hybridization.

A number of allotypic markers of the rabbit immunoglobulins are present only on intact immunoglobulin molecules and not on the isolated heavy or light chains. Some of these markers, although determined by the kappa light chain, are limited to a single heavy chain class of Ig. They can be generated by in vitro hybridization of the kappa light chain with the appropriate heavy chain.

Phylogeny of the rabbit gamma-chain determinants: a d12-like antigenic determinant in Pronolagus rupestris.

A survey for constant region gamma-chain allotypes (de locus) was undertaken in different lagomorph genera. As yet only Pronolagus rupestris, a paleolaginae, showed the presence of a determinant similar to rabbit d12 although it lacked the widespread e15 determinant. All seven individuals possessed the d12 like determinant which was studied by immunodiffusion, haemagglutination inhibition, radiobinding and binding inhibition assays.

Cell differentiation in the presence of cytochalasin B: studies on the "switch" to IgG secretion after polyclonal B cell activation.

Mouse spleen cells were cultured with lipopolysaccharide in conditions that activate both IgM and IgG secretion. Addition of cytochalasin B (CB), an inhibitor of cytokinesis, lead to a high degree of polynucleation, with little effect on Ig secretion. Using cytoplasmic staining with fluorochrome conjugated antisera, we determined the numbers of IgG-containing cells that also contained IgM in their cytoplasm.

Immunoglobulin isoantigens (allotypes) in the mouse : V. Characterization of IgM allotypes.

Murine antisera raised against allogeneic lymphoid cells often contain antibodies to IgM allotypes. Rarely, allotypic antibodies to IgM have been found after immunization withB. pertussis anti-B.

Expression of IgD by murine lymphocytes. Loss of surface IgD indicates maturation of memory B cells.

B lymphocytes capable of generating primary IgM and IgG plaque-forming cells (PFC) responses to burro erythrocytes have surface IgD, as do primary IgM PFC. IgG memroy cells arising after one injection of antigen are divided into two groups, one of which expresses surface IgD while the other has no detectable membrane IgD. PFC generated from the IgG memory cells lacking surface IgD show a higher average avidity than those arising from IgD-positive IgG memory cells, indicating that mature IgG memory cells do not have surface IgD.

Double line phenotypes in rabbit IgG allotypes and their relation to the cis/trans configuration of the IgG markers.

Rabbit antiallotype sera raised against heavy chain markers sometimes show double precipitin lines with all or some of the corresponding antigens (double and single line phenotypes). In a number of cases the double line phenotypes behave as alleles of the single line phenotypes and this feature allows a genetic and immunochemical analysis of these systems. In three cases that have been analysed, the double line phenotype arise when a precipitating a locus allotype and a non-precipitating d or e locus allotype are present on the same molecule (a1 and d14), (a1 and d11), (a3 and d11).

Restriction of the anti-bovine serum albumin response in rabbits immunized with Micrococcus lysodeikticus.

Rabbits capable of producing antibodies of restricted heterogeneity in response to Micrococcus lysodeikticus are equally capable of producing antibodies of restricted heterogeneity to bovine serum albumin. These antibodies are produced when animals are simultaneously injected with micrococcus and BSA and their specificity is restricted to a small number of epitopes. These results suggest that micrococcal vaccines can induce the restriction of heterogeneity in antibodies raised against totally unrelated antigens.

Evidence for quasi-silent germline genes coding for phylogenetically ancient determinants of the rabbit a locus allotypes.

Anti-a2 sera raised in a3 rabbits are shown to detect determinants on a2 molecules which are different from those detected by anti-a2 sera raised in a1 animals. The former determinants are occasionally observed at a low level in rabbits of the a1 allotype and at a high level in sera of Leporidae of different genera. The two types of anti-a2 sera are shown to compete for the same sterical region of the a2 molecules.

[Clinical treatment of inflammatory and benign ulcerous diseases of the stomach and duodenum with a new combination preparation (Aci-Tensilan) (author's transl)].

A gastroduodenal combination preparation was introduced at a deliberately high dosage into a clinical treatment schema. A marked improvement of the subjective symptoms already appeared after a short treatment in hospital, pain in particular being rapidly affected. Younger patients tolerated the preparation excellently, older ones had a marked sedation.

Rheumatoid factor appearance in Micrococcus lysodeikticus immunization and its interference with allotype specific reactions.

Rabbits hyperimmunized with Micrococcus lysodeikticus produce, in addition to anti-cell wall antibodies, considerable amounts of anti-immunoglobulins or rheumatoid factors which can interfere with immune reactions. In particular, in immunodiffusion, they can reveal non precipitating systems for which the immunodiffusion typing sera are usually not tested. In our hands, rabbit allotypes d12 and A8 give rise to visible immunodiffusion reactions in presence of rheumatoid factors.