Publications by authors named "Longyu Zhao"

Rapid advancements in DNA sequencing technologies are providing new approaches for bacterial taxonomy. The genus is a member of the family , which consists of more than 150 genera. In this study, genome sequence analysis was conducted to revisit the taxonomic status of and , the only two species of this genus.

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A Gram-stain negative, rod-shaped, aerobic, oxidase-positive and catalase-weakly positive bacterial strain with polar or subpolar flagellum, designated RZ04, was isolated from an intertidal sand sample collected from a coastal area of the Yellow Sea, China. The organism was observed to grow optimally at 25 °C and pH 6.5-7.

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For the development of safe and effective EBV (Epstein-Barr virus) vaccines, the Ag85A signal peptide from H37Rv was used to construct a recombinant secretory BCG (Bacillus Chalmette-Guérin) plasmid. The Ag85A gene, fused to the EBV LMP2A (latent membrane protein) and hGM-CSF (human granulocyte/macrophage colony-stimulating factor) genes, was inserted into the pMV261 vector (secretory BCG plasmid). The expression levels of the hGM-CSF and LMP2A proteins in rBCG (recombinant BCG) were measured by Western blot analysis.

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A bacterial strain designated RZ03 was isolated from an intertidal sand sample from the Yellow Sea in China and characterised using a polyphasic taxonomic approach. Cells of strain RZ03 were observed to be Gram-stain negative, aerobic, and oxidase and catalase positive rods showing gliding motility and forming yellow colonies. Growth was found to occur at 7-30 °C (optimum, 25 °C), at pH 5.

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The economic losses caused by postharvest fruits diseases have attracted global attention. Traditional chemical fungicide could not meet the need of humans. In recent years, microbial agent which has begun to take the place of chemical fungicide comes into people's vision.

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Background: Findings of recent studies have demonstrated a rapid increase of the incidence of papillary thyroid carcinoma (PTC), which accounts for nearly 80% of thyroid cancers.

Objectives: The aim of this study was to explore the association between AXIN2 gene polymorphism and papillary thyroid carcinoma (PTC).

Patients And Methods: 106 blood samples (56 PTC patients and 50 healthy controls) were drawn from China-Japan Union Hospital in Jilin province, China, during October 2010 to March 2011.

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Article Synopsis
  • The study highlights the need for alternatives to chemical fungicides for combating postharvest fruit diseases, specifically gray mold in pears.
  • Researchers found that Bacillus amyloliquefaciens strain BA3 outperformed other treatments in inhibiting mold growth and positively impacted the fruit's vitamin C content without adversely affecting other quality metrics.
  • The findings suggest that BA3 could serve as an effective biocontrol agent for preserving the quality of pears after harvest.
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Article Synopsis
  • Development of a broad-specificity monoclonal antibody (MAb) for organophosphorus pesticides (OPs) using a specific hapten led to a competitive indirect enzyme-linked immunosorbent assay (ciELISA).
  • The ciELISA exhibited high sensitivity to various OPs, achieving IC50 values between 23.1 and 151.2 ng mL(-1), and demonstrated effective recovery rates from vegetable and fruit samples using a QuEChERS method.
  • This assay, confirmed by gas chromatography-tandem mass spectrometry (GC-MS/MS), proves effective for monitoring OP contamination in agricultural products.
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Objective: To evaluate the association between BRAF(V) 600E mutation and pathological features in papillary thyroid carcinoma (PTC).

Methods: The raleted studies were searched through Medline, PubMed, and Web of Science, and meta-analysis was used to calculate the OR and 95%CI of the study results.

Results: A total of 52 studies including 12 029 PTC patients was identified.

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Objective: Overweight and obesity, indicated as increased body mass index, are associated with the risk of some cancers. We carried out a meta-analysis on published cohort and case-control studies to assess the strength of association between body mass index and gastric cancer.

Methods: Relevant studies were identified through PubMed, Web of Science and Medline electronic databases.

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The BRAF (V600E) mutation is a common genetic alteration in papillary thyroid carcinoma (PTC) and is associated with some pathological features. The association has been widely reported, but results were inconclusive. In this study a meta-analysis was done to explore the association between BRAF(V600E) mutation and pathological features in papillary thyroid carcinoma.

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Objective: To observe the association between childhood leukemia and magnetic field exposure.

Methods: The literature was searched by PubMed, ProQuest, Web of Science (SCI) and Medline databases during 1997-2013. Heterogeneity in several studies was weighted by I-squared value.

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Objective: To examine the effect of exposure to extremely low-frequency electromagnetic fields (ELF EMFs) on the liver function of workers.

Methods: The workers in a factory were selected as subjects, and the recent physical examination data of these workers were collected. The workers aged 20∼40 years and with more than 2 years' working experience were included for analysis; considering the intensity of electromagnetic field, the workers exposed to less electromagnetic radiation were assigned to exposure I group (n = 123), those exposed to more electromagnetic radiation to exposure II group (n = 229), and those not exposed to electromagnetic radiation to control group (n = 212).

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Objective: To observe the effects of extremely low frequency electromagnetic fields (ELF-EMFs) in automotive industry on occupational workers.

Methods: A total of 704 workers were investigated, and 374 workers were chosen and divided into two groups (control group and exposure group) according to the inclusive criteria, namely male with age 20-40 years old and ≥ 2 years of exposure. The intensities of ELF-EMFs and noise were detected with EFA-300 Field Analyzer (Narda company, Pfullingen, Germany) and AWA5610D integrating sound level meter (Hangzhou Aihua Instruments Co.

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The incidence of thyroid cancer has recently experienced a rapid increase in China, and papillary thyroid carcinoma (PTC) accounts for nearly 80% of human thyroid cancers. In the present study, the differential expression of microRNAs (miRNAs) and their target genes were identified in order to analyze the potential roles of miRNAs as biomarkers and in papillary thyroid carcinogenesis. One hundred and twenty-six PTC samples were collected from patients at the China-Japan Union Hospital, China, and the gene/miRNA expression profiles were examined with Illumina BeadChips and verified by real‑time RT-PCR.

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Objective: To observe the exposure levels of extremely low frequency electromagnetic fields in workplaces and to analyze the effects of extremely low frequency electromagnetic radiation on cardiovascular system of occupationally exposed people.

Method: Intensity of electromagnetic fields in two workplaces (control and exposure groups) was detected with EFA-300 frequency electromagnetic field strength tester, and intensity of the noise was detected with AWA5610D integral sound level. The information of health physical indicators of 188 controls and 642 occupationally exposed workers was collected.

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