Immunomagnetic antibody plus aptamer pseudo-DNA nanocatenane followed by rolling circle amplication for highly-sensitive CTC detection.

Biosensing and detecting the rare circulating tumor cells (CTCs) in complex blood samples are a great challenge but necessary for cancer metastasis prevention. Here we show a novel highly-sensitive biosensing system for detecting CTCs in whole blood. The system is composed of Her2-coated immunomagnetic beads and an anti-EpCAM aptamer assembled pseudo-DNA nanocatenane (PDN) for dual targeting and separating CTCs, in conjunction with the rolling circle amplification (RCA) and molecular beacon (MB) system for CTCs signal amplification.

A novel S-nitrosocaptopril monohydrate for pulmonary arterial hypertension: HO and -SNO intermolecular stabilization chemistry.

S-nitrosocaptopril (CapNO) possesses dual capacities of both Captopril and an NO donor with enhanced efficacy and reduced side effects. CapNO crystals are difficult to make due to its unstable S-NO bond. Here, we report a novel stable S-nitrosocaptopril monohydrate (CapNO·HO) that is stabilized by intermolecular five-membered structure, where one H of HO forms a hydrogen bond with O of the stable resonance zwitterion Cap-S=N-O, and the O in HO forms the dipole-dipole interaction with S through two unpaired electrons.

In vivo inhibition of circulating tumor cells by two apoptosis-promoting circular aptamers with enhanced specificity.

Circulating tumor cells (CTCs) are known as the root cause of cancer metastasis that accounts for 90% of cancer death. Owing to the rarity of blood CTCs and their microenvironmental complexity, the existing biotechnology could not precisely capture and apoptosize CTCs in vivo for cancer metastasis prevention. Here, we designed two double strand circular aptamers aimed to simultaneously target MUC1 and HER2 surface biomarkers on mesenchymal cancer cells.

A carrier-free dual-drug nanodelivery system functionalized with aptamer specific targeting HER2-overexpressing cancer cells.

Exploration of a green carrier to avoid potential systemic toxicity and the unclear metabolic mechanism of traditional nanocarriers is of high importance for cancer therapy. Hence, we developed a carrier-free nanosystem for co-delivery of dual anti-cancer drugs ursolic acid (UA) and doxorubicin (DOX) using a "green" and simple method. The co-assembled nanodrug was further modified with a HER2 aptamer by electrostatic interactions.