Preparation and Thermal Properties of Modified CuO/Polypropylene (PP) Composite.

A uniform, monodispersed superfine cuprous oxide (CuO) sphere with a mean diameter of 850 nm has been synthesized by solution reduction. The study reported the synthesis and thermal properties of CuO/PP composites for the first time. The surface modification of the superfine CuO sphere was carried out by using a silane coupling agent KH-570.

[Study on the public health emergent events in Fujian province in 2004--2007].

Objective: To study the public health emergent events (PHEE) in Fujian province, from 2004 to 2007.

Methods: Descriptive and analytic methods were used to analyze the PHEE in Fujian province according to the internet-based surveillance reports.

Results: From 2004 to 2007, there were 304 emergency events being surveyed.

Clinical characteristics of 26 human cases of highly pathogenic avian influenza A (H5N1) virus infection in China.

Background: While human cases of highly pathogenic avian influenza A (H5N1) virus infection continue to increase globally, available clinical data on H5N1 cases are limited. We conducted a retrospective study of 26 confirmed human H5N1 cases identified through surveillance in China from October 2005 through April 2008.

Methodology/principal Findings: Data were collected from hospital medical records of H5N1 cases and analyzed.

Direct synthesis of ultralong carbon nanotube bundles by spray pyrolysis and investigation of growth mechanism.

A mass of ultralong aligned carbon nanotube (CNT) bundles up to about 8 mm in length was synthesized by utilizing the spray pyrolysis of ferrocene/xylene solutions in the absence of promoters, such as thiophene or pure sulfur, and etching agents, such as plasma, oxygen, ethanol or water. Scanning electron microscopy (SEM) and high-resolution transmission electron microscopy (HRTEM) results indicated the continuous and multi-walled nanotube structure for the CNTs in the macroscopical bundles. A graph demonstrating the correlation between growth time and length of CNT arrays revealed that the maximum growth rate reaches approximately 4612 nm s(-1).

[DNA amplification of Plasmodium vivax parasites from Giemsa-stained blood smears].

Objective: To develop methods of extracting DNA from malaria parasites on Giemsa-stained blood smears.

Methods: Improved Na2HPO4 method and Chelex-100 ion-exchange technique were used to extract DNA from Giemsa-stained or unstained blood smears. Nested PCR was employed for amplification and identification of allelotype in the Plasmodium vivax merozoite surface protein-1 (PvMSP-1).

[Study on the epidemiology and etiologic agent of Dengue fever outbreaks in Fuzhou in 2004].

Objective: To study the epidemiology and etiologic characteristics of a Dengue fever outbreak in Fuzhou from the beginning of September to the end of October in 2004 in order to understand the source of infection.

Methods: Data on descriptive epidemiology was collected to study the characteristics and related factors to the epidemic. Dengue virus was isolated through the use of C6/36 cell line while viral serotypes were identified by indirect immunofluorecent assay with type-specific monoclonal antibody.

Immunochromatographic test for simultaneous serodiagnosis of Babesia caballi and B. equi infections in horses.

An immunochromatographic test for the simultaneous detection of Babesia caballi- and B. equi-specific antibodies (BceICT) was developed using a recombinant B. caballi 48-kDa rhoptry protein (rBc48) and a recombinant truncated B.

[Survey on the current status of important human parasitic infections in Fujian province].

Objective: To understand the prevalence of important human parasitic diseases in Fujian province.

Methods: According to the national guidelines, clustered random sampling was made for the survey. Modified Kato-Katz thick smear was applied to examine helminth eggs for soil-transmitted nematodes and Clonorchis sinensis.

[Study on the timeliness of the notifiable communicable diseases surveillance system in Fujian province, China, 2004].

Objective: To understand the timeliness of the notifiable communicable diseases surveillance system in Fujian province.

Methods: Database from the internet based communicable diseases reporting system was used.

Results: The 50th percentile of time between the disease diagnosed and report recorded in medical faculties was 1 day in 2004 which was 6 days less than that in 2001 - 2003.

[Genotype detection of the merozoite surface protein alleles of Plasmodium vivax].

Objective: To develop a method for detecting the genotype of Plasmodium vivax merozoite surface protein 1 (PvMSP-1) alleles.

Methods: According to the sequence characteristic of PvMSP-1, nested polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was used to amplify the polymorphic region of ICB5-ICB6 which contains Q repeats and PvuII restriction site (Sal-1 type). The PCR product was digested by PvuII restriction endonuclease and the digested fragments were observed by 2% agarose gel electrophoresis.

[Morphological observation of Haplorchis pumilio].

Objective: To provide the basis for the differentiation with similar species of intestinal flukes through observing the figure of Haplorchis pumilio.

Methods: Adults of H. pumilio were collected from the intestine of the cat which was infected with the encysted cercariae of H.

Development of an immunochromatographic test with recombinant EMA-2 for the rapid detection of antibodies against Babesia equi in horses.

An immunochromatographic test (BeICT) for the rapid detection of antibodies against Babesia equi was developed. It clearly differentiated B. equi-infected horses from B.

Rapid immunochromatographic test using recombinant SAG2 for detection of antibodies against Toxoplasma gondii in cats.

An immunochromatographic test using recombinant truncated surface antigen 2 for detection of antibodies against Toxoplasma gondii was developed. Evaluation of detection of the antibody in mice and cats suggests that this test is rapid, simple, accurate, relatively inexpensive, and suitable for use under field conditions.

High-level expression and purification of a truncated merozoite antigen-2 of Babesia equi in Escherichia coli and its potential for immunodiagnosis.

The gene encoding a truncated merozoite antigen-2 (EMA-2t) of Babesia equi was cloned and highly expressed in Escherichia coli as a glutathione S-transferase fusion protein (G-rEMA-2t). Both G-rEMA-2t and rEMA-2t (after the removal of glutathione S-transferase) had good antigenicity. Either Western blot analysis with rEMA-2t or enzyme-linked immunosorbent assay (ELISA) with G-rEMA-2t clearly discriminated the sera of horses experimentally infected with B.