Study on Rapid Detection Technology of Illegal Chemical Compounds Added to External Poultices.

Topical poultices and applied medical devices that can relieve a variety of cervical vertebrae and lumbar vertebrae pain may have illegal additives of pharmaceutical drugs or analogues have additional health risks, but customers may not be aware of what they are using. National Medical Products Administration reviewed and approved "Supplementary test method for identification and content determination of 17 chemicals in affixed medical devices" drafted by Shandong Provincial Institute of Medical Devices and Drug Packaging Inspection in January 2022, but the test method is simple in sample processing and difficult to extract the chemical components in the paste that may cause the phenomenon of missing detection of most illegal added chemical drugs. In this work, the sample handling and extraction methods have been improved and optimized, the content of illegal added chemicals in 47 batches of affixed medical devices was determined by HPLC-MS/MS method and was detected in 37 batches, and the non-steroidal anti-inflammatory drug nimesulide and aspirin were detected in most samples.

Luciferase assay to screen tumour-specific promoters in lung cancer.

Objective: Specific promoters could improve efficiency and ensure the safety of gene therapy. The aim of our study was to screen examples for lung cancer.

Methods: The firefly luciferase gene was used as a reporter, and promoters based on serum markers of lung cancer were cloned.