Pathogenic bioaerosols are critical for outbreaks of airborne disease; however, rapidly and accurately identifying pathogens directly from complex air environments remains highly challenging. We present an advanced method that combines open-set deep learning (OSDL) with single-cell Raman spectroscopy to identify pathogens in real-world air containing diverse unknown indigenous bacteria that cannot be fully included in training sets. To test and further enhance identification, we constructed the Raman datasets of aerosolized bacteria.
View Article and Find Full Text PDFCompr Rev Food Sci Food Saf
July 2024
Application of agricultural waste such as rapeseed meal (RM) is regarded as a sustainable way to improve soil phosphorus (P) availability by direct nutrient supply and stimulation of native phosphate-solubilizing microorganisms (PSMs) in soils. However, exploration of the in situ microbial P solubilizing function in soils remains a challenge. Here, by applying both phenotype-based single-cell Raman with DO labeling (Raman-DO) and genotype-based high-throughput chips targeting carbon, nitrogen and P (CNP) functional genes, the effect of RM application on microbial P solubilization in three typical farmland soils was investigated.
View Article and Find Full Text PDFAntibiotic resistance is an escalating global concern, leading to millions of annual deaths worldwide. Human activities can impact antibiotic resistance gene (ARG) prevalence in aquatic ecosystems, but the intricate interplay between anthropogenic disturbances and river system resilience, and their respective contributions to the dynamics of different river segments, remains poorly understood. In this study, we investigate the antibiotic resistome and microbiome in water and sediment samples from two distinct sub-watersheds within a specific watershed.
View Article and Find Full Text PDFLong-term exposure to the indoor environment may pose threats to human health due to the presence of pathogenic bacteria and their byproducts. Nanoscale extracellular vesicles (EVs) extensively secreted from pathogenic bacteria can traverse biological barriers and affect physio-pathological processes. However, the potential health impact of EVs from indoor dust and the underlying mechanisms remain largely unexplored.
View Article and Find Full Text PDFMicroplastics (MPs) ubiquitous in environments promote the dissemination of antibiotic resistance genes (ARGs), threatening ecosystem safety and human health. However, quantitative assessments of the health risks of ARGs (HRA) in plastisphere and an in-depth exploration of their driving mechanisms are still lacking. Here, the microbiomes, ARGs, and community assembly processes of five types of MPs in an urbanizing watershed were systematically investigated.
View Article and Find Full Text PDFUnderstanding evolution of antibiotic resistance is vital for containing its global spread. Yet our ability to in situ track highly heterogeneous and dynamic evolution is very limited. Here, we present a new single-cell approach integrating D O-labeled Raman spectroscopy, advanced multivariate analysis, and genotypic profiling to in situ track physiological evolution trajectory toward resistance.
View Article and Find Full Text PDFEnviron Sci Ecotechnol
January 2023
Antibiotic resistance genes (ARGs) are a well-known environmental concern. Yet, limited knowledge exists on the fate and transport of ARGs in deep freshwater reservoirs experiencing seasonal hydrological changes, especially in the context of particle-attached (PA) and free-living (FL) lifestyles. Here, the ARG profiles were examined using high-throughput quantitative PCR in PA and FL lifestyles during four seasons representing two hydrological phenomena (vertical mixing and thermal stratification) in the Shuikou Reservoir (SR), Southern China.
View Article and Find Full Text PDFMicroplastics (MPs) are emerging as anthropogenic vectors for the colonization and transportation of microbial communities in aquatic ecosystems. However, the composition of the microbiome and its environmental risk on field MPs at watershed scale has rarely been explored. Here, geographical distributions of microbiome, antibiotic resistance genes (ARGs) and virulence factors (VFs) on field MPs at watershed scale were characterized and their potential environmental risks were evaluated based on the data from metagenomic analyzes.
View Article and Find Full Text PDFUrbanization is a process of ecosystem evolution mediated by human activities. One of the main consequences is the alteration of antibiotic resistome and microbiome in aquatic environment, which may transfer from water to sediments and exert a long-term health concern to aquatic animals and even humans. However, the role of urbanization in shaping resistome and microbiome in water and sediments is largely unknown.
View Article and Find Full Text PDFMicroplastics (MPs) have been considered as a new vector for the long-distance transport of pathogens in aquatic ecosystems. However, the composition of viral communities attached on MPs and their environmental risk are largely unknown. Here, we profiled the viral diversity and potential risk in five different MPs collected from the Beilun River based on metagenomic analysis.
View Article and Find Full Text PDFMicroplastics (MPs) are becoming ubiquitous in environments and viewed as carriers of antibiotic resistance genes (ARGs). Rivers connecting differently urbanized areas contribute a significant input of MPs and ARGs to the environment. However, a systematic study assessing the role of urbanization in shaping antibiotic resistome and mobilome in riverine MPs is lacking.
View Article and Find Full Text PDFThe background noise (BGN) of test equipment affects the lower detection limit of the measurement range, and achieving an ultralow BGN is identified as a formidable challenge for highly accurate testing of a free-standing film. In this paper, a new gas transmission rate test apparatus is designed by detecting the electrical resistance increase with calcium corrosion. Thanks to the new design strategy, the only permeable place of this apparatus is the clamping and holding site.
View Article and Find Full Text PDFHeavy metals (HM) pollution exerts an effect on microbial community composition and structure during composting, the way how microbial community responses to HM pressure is remain poorly understood though. The aim of this study was to explore functional roles of microorganisms based on selective pressures of HM (Cu, Zn and Cd). The results of microbial resistance showed that the toxicity of metals to microorganisms were Cu > Zn > Cd during composting.
View Article and Find Full Text PDFThe goal of this work was to explore the effect of Fenton pretreatment combined with bacteria inoculation on the formation of humic substances (HS) during rice straw composting. In this study, the compound bacterial agents were inoculated after Fenton pretreatment during rice straw composting. The results suggested that the coupling effects of Fenton pretreatment and bacteria inoculation promoted the humification process, which might be the reason of organic fractions degradation and transformation.
View Article and Find Full Text PDFHumic substance (HS), as an aromatic compound, is the core product of aerobic fermentation. Denitrification-dependent degradation of aromatic compounds have been repeatedly observed in environment. However, few studies have elucidated the relationship between denitrification and aromatic HS during sludge aerobic fermentation.
View Article and Find Full Text PDFTransformation of DOM fractions with different molecular weights during composting of chicken manure (CM), garden waste (GW) and municipal solid waste (MSW) was evaluated in this study. The results revealed that DOM concentrations decreased by 49.8%, 53.
View Article and Find Full Text PDFThis study aimed to compare the effects of biochar, montmorillonite and their mixture on nitrogen availability and nitrogen loss during chicken manure composting. Four lab-scale composting experiments, the control (CK), 5% biochar addition (BC), 5% montmorillonite addition (M) and 2.5% biochar + 2.
View Article and Find Full Text PDFThe aim of this study was to explore the effects of adding biochar and montmorillonite alone on the correlations between bacteria and bioavailability of heavy metals (HM) during chicken manure composting. Three composting experiments were conducted, containing the without ameliorant, 10% biochar and 10% montmorillonite. The results showed that biochar and montmorillonite ameliorants significantly reduced the bioavailability of Cu by 90.
View Article and Find Full Text PDFThe aim of this study is to reduce nitrogen (N) loss and investigate the role of ammonia (NH) recycling on N fractions, environmental factors and bacterial communities. In this study, collected NH from composting and recycled in it. The results showed that NH recycling affected N-cycling processes such as nitrification.
View Article and Find Full Text PDFThe aim of this study was to remove heavy metals from composting system using a novel method combined functional bacteria with adsorbent materials. Four types of adsorbent materials were selected in this study. Results showed that Cr had significant removal efficiency, especially in sponge treatment (19.
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