High frequency regeneration of plants via callus-mediated organogenesis from cotyledon and hypocotyl cultures in a multipurpose tropical tree (Neolamarkia Cadamba).

In this works, a simple, efficient and repeatable protocol was developed for in vitro regeneration via callus-mediated organogenesis of Neolamarkia Cadamba using cotyledonary petioles and hypocotyls. Effects of basal medium, plant growth regulators, the types and age of explant on the formation of adventitious buds/shoots were studied. Meanwhile, histological analysis for early ontogenic stages and genetic stability assessment by flow cytometry were investigated.

Cloning and characterization of squalene synthase and cycloartenol synthase from .

Mogrosides and steroid saponins are tetracyclic triterpenoids found in . Squalene synthase (SQS) and cycloartenol synthase (CAS) are key enzymes in triterpenoid and steroid biosynthesis. In this study, full-length cDNAs of and were cloned by a rapid amplification of cDNA-ends with polymerase chain reaction (RACE-PCR) approach.

Transcriptome Analysis of Taxillusi chinensis (DC.) Danser Seeds in Response to Water Loss.

Background: Taxillus chinensis (DC.) Danser, the official species of parasitic loranthus that grows by parasitizing other plants, is used in various traditional Chinese medicine prescriptions. ABA-dependent and ABA-independent pathways are two major pathways in response to drought stress for plants and some genes have been reported to play a key role during the dehydration including dehydration-responsive protein RD22, late embryogenesis abundant (LEA) proteins, and various transcription factors (TFs) like MYB and WRKY.

[Study on exogenous hormones inducing parthenocarpy fruit growth and development and quality of Siraitia grosvenorii].

To explore the growth and development and analyze the quality of the parthenocarpy fruit induced by exogenous hormones of Siraitia grosvenorii. the horizontal and vertical diameter, volume of the fruit were respectively measured by morphological and the content of endogenous hormones were determined by ELISA. The size and seed and content of mogrosides of mature fruit were determined.

Identification of novel and conserved microRNAs in Panax notoginseng roots by high-throughput sequencing.

Background: MicroRNAs (miRNAs) are small, non-coding RNAs that are important regulators of gene expression, and play major roles in plant development and their response to the environment. Root extracts from Panax notoginseng contain triterpene saponins as their principal bioactive constituent, and demonstrate medicinal properties. To investigate the novel and conserved miRNAs in P.

[Effect of Nutrient Solution in Soilless Cultivation on Growth, Antioxidase Activities and Amino Acid Contents of Nervilia fordii].

Objective: To select the best formula for soilless cultivation of Nervilia fordii.

Methods: Four different formulas of nutrient solution on growth,antioxidase activities and amino acid contents were investigated.

Results: The concentration of 1/2 lettuce nutrient solution formula was the most propitious for the growth of Nervilia fordii, the antioxidase activities and amino acid contents were increased in the treatment of nutrient solution,and the highest amino acid content was observed in 1/2 dose of green leafy vegetables nutrient solution formula.

[Rapid propagation system for tissure culture of Smilax glabra].

Objective: Establishing the rapid propagation for plant tissue culture of Smilax glabra in order to rationally protect and utilize the resource.

Methods: The seed, shoot, leaf and rhizome of Similax glabra were cultured as explants. Different media and plant growth regulators at different concerntration were used.

[Extraction of total RNA and cloning of sgDHAR gene from Siraitia grosvenorii].

Total RNA was isolated from Siraitia grosvenorii fruit by the method of modified Trizol, according to S. grosvenorii fruit characteristics of rich phenols, polysaccharide, oil and proteins. The OD260/280, OD260/230, RNA integrity (RIN) and yield of the total RNA with this method were 2.

[Study on quantitative assessment of Siraitia grosvenorii germplasms by general index].

Objective: To establish a weight-marking method and evaluate Siraitia grosvenorii germplasms.

Method: The characters of 21 kinds of S. grosvenorii germplasms in the field and the lab were analyzed, seven important characters were selected to weight the marks.

[Progress on research of tissue culture of Siraitia grosvenorii].

In this paper we reviewed the development of tissue culture, current situations of virus-free plantlets industrialization and the way to deal with the situations, application prospects of Siraitia grosvenorii so as to give some advice for its further study and application.