Publications by authors named "Longfei Hu"

The Qilian Mountain Area (QMA) serves as a crucial ecological barrier and strategic water conservation zone in China. Recent years have seen heightened social attention to environmental issues within the QMA, underscoring the need for accurate and continuous land cover maps to support ecological monitoring, analysis, and forecasting. This paper presents the QMA_LC30 dataset, which includes 9 land cover categories and spans the period from 1990 to 2020, with updates every 5 years.

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The exposure risk of plasmid-mediated quinolone resistance (PMQR) genes increases the incidence of resistant bacterial infections, has resulted in clinical treatment failures with ciprofloxacin, necessitating urgent implementation of novel strategies for controlling this situation. Matrine serves as the principal constituent of the traditional Chinese herb Sophora flavescens Ait. and exhibits pharmacological activities including anti-inflammatory, antibacterial, anti-tumor, and hepatoprotective effects.

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Objective: Maintenance therapy following first-line chemotherapy is of particular significance in patients diagnosed with recurrent or metastatic nasopharyngeal carcinoma (NPC). We conducted a meta-analysis to investigate the impact of maintenance therapy (MT) on the survival prognosis of individuals with recurrent or metastatic NPC.

Methods: The databases Embase, PubMed, and the Cochrane Library were thoroughly searched in a comprehensive manner.

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Cations such as K play a key part in the CO electroreduction reaction, but their role in the reaction mechanism is still in debate. Here, we use a highly symmetric Ni-N structure to selectively probe the mechanistic influence of K and identify its interaction with chemisorbed CO. Our electrochemical kinetics study finds a shift in the rate-determining step in the presence of K.

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Article Synopsis
  • Understanding the dealloying process is essential for creating effective alloy catalysts for the oxygen reduction reaction (ORR), but the specific steps involved are not fully understood.
  • Using X-ray absorption fine structure spectroscopy, researchers studied the dealloying of a two-dimensional ordered alloy called PtTe, which is known for its strong ORR performance.
  • The study finds that as Pt transforms structurally before Te is completely removed, a defect-rich Pt catalyst is formed, significantly improving ORR activity compared to standard commercial Pt/C catalysts.
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Article Synopsis
  • The study presents a method for creating a core-shell catalyst with a high-index Pt shell and a PtCu intermetallic core, enhancing the efficiency of oxygen reduction reactions without high temperatures.
  • Using surfactant effects and solvent-ligand interactions, the catalyst is formed through a self-assembly process that directly fabricates the desired structure.
  • The catalyst demonstrates a high performance with a half-wave potential of 0.911 V and maintains its effectiveness even after 15,000 cycles, largely due to improved electronic interactions that stabilize key reaction intermediates.
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Single-cell transcriptome sequencing (scRNA-seq) can resolve the expression characteristics of cells in tissues with single-cell precision, enabling researchers to quantify cellular heterogeneity within populations with higher resolution, revealing potentially heterogeneous cell populations and the dynamics of complex tissues. However, the presence of a large number of technical zeros in scRNA-seq data will have an impact on downstream analysis of cell clustering, differential genes, cell annotation, and pseudotime, hindering the discovery of meaningful biological signals. The main idea to solve this problem is to make use of the potential correlation between cells and genes, and to impute the technical zeros through the observed data.

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The assessment of land cover and changes will help to understand the temporal and spatial pattern of land cover in the world and the Belt and Road (B&R) region, and provide reference information for global sustainable development and the Belt and Road construction. In this paper, the 1 km global land cover classification maps of 2016 and 2020 with a high accuracy of 88% are mapped using the Moderate Resolution Imaging Spectroradiometer (MODIS) time series surface reflectance products. Based on the maps, the land cover status of the world and the Belt and Road region, the land cover change from 2016 to 2020, and the mutual transformation characteristics between various types, are analyzed.

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Fibrosis, angiogenesis and chronic inflammation are the inherent characteristics of endometriosis (EMS). The cellular heterogeneity of ectopic and non-ectopic endometrium by single-cell RNA-sequencing (scRNA-seq)at secretory phase without the disturbance of hormone drugs hasn't been explored so far. In this study, scRNA-seq was adopted to explore the properties of ectopic endometrium (ECE), eutopic endometrium (EUE) and normal endometrium (NOE) at secretory phase.

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As an indicator of clinical prognosis, lymph node metastasis of breast cancer has drawn great attention. Many reports have revealed the characteristics of metastatic breast cancer cells, however, the effect of breast cancer cells on the microenvironment components of lymph nodes and spatial transcriptome atlas remains unclear. In this study, by integrating single-cell RNA sequencing (scRNA-seq) and spatial transcriptomics, we investigate the transcriptional profiling of six surgically excised lymph node samples and the spatial organization of one positive lymph node.

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Molecular mechanisms underlying breast cancer lymph node metastasis remain unclear. Using single-cell sequencing, we investigated the transcriptome profile of 96,796 single cells from 15 paired samples of primary tumors and axillary lymph nodes. We identified nine cancer cell subclusters including CD44 + / ALDH2 + /ALDH6A1 + breast cancer stem cells (BCSCs), which had a copy-number variants profile similar to that of normal breast tissue.

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In this study, pentamode metamaterials were proposed for thermal stress accommodation of alkali metal heat pipes. Sodium/Inconel 718 heat pipes with and without pentamode metamaterial reinforcement were designed and fabricated. Then, these heat pipes were characterized by startup tests and thermal response simulations.

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Background: Since 2019, a novel coronavirus named 2019 novel coronavirus (2019-nCoV) has emerged worldwide. Apart from fever and respiratory complications, acute kidney injury has been observed in a few patients with coronavirus disease 2019. Furthermore, according to recent findings, the virus has been detected in urine.

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The potentially different genetics and epigenetics in the primary tumors and metastases affect the efficacy of treatment in breast cancer patients. Nevertheless, the cellular and molecular mechanisms of breast cancer lymph node metastasis still remain elusive. Here, we employed single-cell RNA sequencing to acquire the transcriptomic profiles of individual cells from primary tumors, negative lymph nodes (NLs) and positive lymph nodes (PLs).

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During human spermatogenesis, germ cells undergo dynamic changes in chromatin organization/re-packaging and in transcriptomes. In order to better understand the underlying mechanism(s), scATAC-Seq of 5376 testicular cells from 3 normal men were performed. Data were analyzed in parallel with the scRNA-Seq data of human testicular cells.

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A substantial number of male infertility is caused by azoospermia. However, the underlying etiology and the molecular basis remain largely unknown. Through single-cell (sc)RNA sequencing, we had analyzed testis biopsy samples from two patients with obstructive azoospermia (OA) and nonobstructive azoospermia (NOA).

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Purpose: The emergence of plasmid-mediated quinolone resistance (PMQR) is a global challenge in the treatment of clinical disease in both humans and animals and is exacerbated by the presence of different PMQR genes existing in the same bacterial strain. Here, we discovered that a natural isoquinoline alkaloid palmatine extracted from traditional Chinese medicinal plants effectively inhibited the activity of PMQR proteins QnrS and AAC(6')-Ib-cr.

Methods: In total 120 clinical ciprofloxacin-resistant () were screened for the presence of and by PCR.

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Purpose: To determine associations between genomic DNA methylation in testicular cells and azoospermia in human males.

Methods: This was a case-control study investigating the differences and conservations in DNA methylation, genome-wide DNA methylation, and bulk RNA-Seq for transcriptome profiling using testicular biopsy tissues from NOA and OA patients. Differential methylation and different conserved methylation regions associated with azoospermia were identified by comparing genomic DNA methylation of testicular seminiferous cells derived from NOA and OA patients.

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Nephrotoxicity is the major limiting factor for the clinical use of vancomycin (VCM) for treatment of serious infections caused by multiresistant Gram-positive bacteria. This study investigated the renal protective activity of rutin in a rat model of VCM-induced kidney injury in male Wistar rats. VCM administered intraperitoneally at 200 mg/kg twice daily for 7 successive days resulted in significant elevation of blood urea nitrogen and creatinine, as well as urinary -acetyl-β-D-glucosaminidase.

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Background: More than a decade after the outbreak of human coronaviruses (HCoVs) SARS in Guangdong province and Hong Kong SAR of China in 2002, there is still no reoccurrence, but the evolution and recombination of the coronaviruses in this region are still unknown. Therefore, surveillance on the prevalence and the virus variation of HCoVs circulation in this region is conducted.

Methods: A total of 3298 nasopharyngeal swabs samples were collected from cross-border children (<6 years, crossing border between Southern China and Hong Kong SAR) showing symptoms of respiratory tract infection, such as fever (body temperature > 37.

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Background: To address increasing tuberculosis (TB) incidence in foreign-born populations, immigrant TB screening programs have been implemented in the USA. These programs are modified periodically, the effectiveness of which have been disputed. The aim of this retrospective study was to assess the value of the 2009 Technical Instructions for Tuberculosis Screening and Treatment Using Cultures and Directly Observed Therapy (CDOT TB TI) in a cohort of the USA permanent-resident applicants from China.

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Emberiza elegans is a common bunting with very wide geographical distribution. In this paper, the complete mitochondrial genome of E. elegans (16,779 bp in length) was analyzed for building the database.

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Emberiza rustica, least concern species (IUCN), is a passerine bird in the bunting family with wide geographical range. The complete mitochondrial genome of E. rustica (16,798 bp in length) had been analyzed for building the database.

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Black-faced bunting (Emberiza spodocephala) is a common small songbird with widely geographical range. In this study, the complete mitochondrial genome of the Emberiza spodocephala was determined. The study showed that the total length of the mitogenome was 16,796 bp as a circular DNA consists of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and 1 control region (CR).

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