CRISPR/Cas12a and recombinase polymerase amplification-based rapid on-site nucleic acid detection of duck circovirus.

Background: Duck circovirus (DuCV) infections commonly induce immunosuppression and secondary infections in ducks, resulting in significant economic losses in the duck breeding industry. Currently, effective vaccines and treatments for DuCV have been lacking. Therefore, rapid, specific, and sensitive detection methods are crucial for preventing and controlling DuCV.

Specific detection of duck adeno-associated virus using a TaqMan-based real-time PCR assay.

Duck adeno-associated Virus (DAAV) is a novel pathogen that was recently discovered in ducks. To establish a molecular detection assay for DAAV for further epidemiological investigation and pathogenic mechanism. Here, we designed specific primers and probes according to the sequence characteristics of the newly discovered DAAV and then established a TaqMan real-time PCR method (TaqMan-qPCR) for the detection of DAAV.

Specific detection of pigeon parvovirus with TaqMan real-time PCR technology.

The viruses of the Parvoviridae family can infect both vertebrate and invertebrate animals. Recently, pigeon parvovirus (PiPV) was detected in the feces of wild urban pigeons. Owing to no specific detection platform for PiPV, studies on the epidemiology of PiPV are still a research gap.

Fibroblast growth factor 8 promotes in vitro neurite outgrowth of placode-derived petrosal and nodose ganglia to varying degrees.

Fibroblast growth factors (FGFs) are required for the specification and formation of the epibranchial placodes, which give rise to the distal part of the cranial sensory ganglia. However, it remains unclear whether FGFs play a role in regulating the neurite outgrowth of the epibranchial placode-derived ganglia during further development. Previous studies have shown that Fibroblast growth factor 8 (FGF8) promotes neurite outgrowth from the statoacoustic ganglion in vitro.

Development of the first officially licensed live attenuated duck hepatitis A virus type 3 vaccine strain HB80 in China and its protective efficacy against DHAV-3 infection in ducks.

Duck hepatitis A virus type 3 (DHAV-3) is an infectious virus that is highly fatal to ducklings and causes significant economic losses in the duck industry worldwide. Biosecurity and vaccination are required to control the pathogen. In the present study, we attenuated a lowly pathogenic DHAV-3 clinical isolate, named as HB, by serial passaging in duck embryos, and followed by several adaptive proliferations in specific-pathogen-free (SPF) chicken embryos.

Analysis of deformation mechanism of rainfall-induced landslide in the Three Gorges Reservoir Area: Piansongshu landslide.

The Three Gorges Reservoir Area (TGRA) is characterized by unique geological features that increase its susceptibility to landslides. These slopes are especially prone to destabilization when influenced by external triggers like rainfall. This research focuses on the Piansongshu landslide within the TGRA, aiming at unraveling the complex internal deformation mechanisms of landslides triggered by rainfall and providing critical insights for their prevention and mitigation.

Genomic analysis reveals the population structure and antimicrobial resistance of avian Pasteurella multocida in China.

Objectives: To investigate the population structure and antimicrobial resistance (AMR) of avian Pasteurella multocida in China.

Methods: Utilizing WGS analysis, we explored the phylogeny using a dataset of 546 genomes, comprising avian P. multocida isolates from China (n = 121), the USA (n = 165), Australia(n = 153), Bangladesh (n = 3) and isolates of other hosts from China (n = 104).

Influences of dynamic load phase shifts on the energetics and biomechanics of humans.

Using load-suspended backpacks to reduce vertical peak dynamic load exerted on humans can reduce metabolic costs. However, is it possible to further reduce metabolic cost by modulating dynamic load phase shift? If so, is anti-phase better than the others? In this study, we investigated the biomechanics, energetics and trunk response under phase shifts. Nine subjects wearing an active backpack with 19.

Inducing factors and deformation mechanism of the Zhangjiacitang landslide in the Three Gorges Reservoir Area.

Landslides are the most widely distributed geological hazards in the Three Gorges Reservoir Area (TGRA). Understanding the deformation mechanism and evolution of landslides is of great significance for their prevention and control. In this study, we focused on the Zhangjiacitang landslide, a typical bank landslide in the TGRA.

Exploring the effects of skeletal architecture and muscle properties on bipedal standing in the common chimpanzee () from the perspective of biomechanics.

It is well known that the common chimpanzee, as both the closest living relative to humans and a facultative bipedal, has the capability of bipedal standing but cannot do so fully upright. Accordingly, they have been of exceeding significance in elucidating the evolution of human bipedalism. There are many reasons why the common chimpanzee can only stand with its hips-knees bent, such as the distally oriented long ischial tubercle and the almost absent lumbar lordosis.

Numerical and Field Measurement Investigation on Foundation Pit Excavation Blasting of Anchor in Suspension Bridge.

The foundation pit of a suspension bridge project in the Three Gorges Reservoir area is investigated in this paper. The pit is located under an unstable rock mass and landslide body; its base lithology is mudstone. The bridge foundation pit project mainly adopts blasting excavation to accelerate construction progress.

Isolation and Functional Analysis of , a WRKY Transcription Factor Gene, with Functions in Tolerance to Cold and Salt Stress in Transgenic .

The grape ( L.) not only has a long history of cultivation, but also has rich nutritional value and high economic value. However, grapes often face many threats in the growth process.

Tunnel collapse risk assessment based on improved quantitative theory III and EW-AHP coupling weight.

It is a multi-criteria decision issue to conduct a risk assessment of the tunnel. In this paper, a tunnel collapse risk assessment model based on the improved theory of quantification III and the fuzzy comprehensive evaluation method is proposed. According to the geological conditions and the construction disturbance classification method, the evaluation factors are selected, and the tunnel collapse risk level is divided into 5 levels according to the principle of maximum membership degree.

Characterization and Categorization of Various Human Lower Limb Movements Based on Kinematic Synergies.

A proper movement categorization reduces the complexity of understanding or reproducing human movements in fields such as physiology, rehabilitation, and robotics, through partitioning a wide variety of human movements into representative sub-motion groups. However, how to establish a categorization (especially a quantitative categorization) for various human lower limb movements is rarely investigated in literature and remains challenging due to the diversity and complexity of the lower limb movements (diverse gait modes and interaction styles with the environment). Here we present a quantitative categorization for the various lower limb movements.

WITHDRAWN: Molecular Evolution and Amino Acid Characteristics of Main Antigen Genes of Clinical Duck-Derived H5N6 Subtype Avian Influenza Virus in East China from 2015 to 2019.

This article has been withdrawn at the request of the authors. The Publisher apologizes for any inconvenience this may cause.

Phosphorylation of CRMP2 by Cdk5 Negatively Regulates the Surface Delivery and Synaptic Function of AMPA Receptors.

AMPA receptor mediate most fast excitatory synaptic transmission and play a key role in synaptic plasticity in the central nervous system (CNS) by trafficking and targeting of its subunits to individual postsynaptic membrane. Collapsing response mediator protein 2 (CRMP2), an intracellular phospho-protein, has been reported to promote the maturation of the dendritic spine and transfer AMPA receptors to the membrane. However, our knowledge about the molecular mechanisms of CRMP2 regulating AMPA receptors trafficking is limited.

A portable exotendon assisting hip and knee joints reduces muscular burden during walking.

Assistive devices are used to reduce human effort during locomotion with increasing success. More assistance strategies are worth exploring, so we aimed to design a lightweight biarticular device with well-chosen parameters to reduce muscle effort. Based on the experience of previous success, we designed an exotendon to assist in swing leg deceleration.

Differential metabolism-associated gene expression of duck pancreatic cells in response to two strains of duck hepatitis A virus type 1.

Several outbreaks of duck hepatitis A virus type 1 (DHAV-1), which were characterized by yellow coloration and hemorrhage in pancreatic tissues, have occurred in China. The causative agent is called pancreatitis-associated DHAV-1. The mechanisms involved in pancreatitis-associated DHAV-1 infection are still unclear.

[Analysis of genetic variant in a fetus featuring pontocerebellar hypoplasia type 6].

Objective: To explore the genetic basis for a fetus with cerebellar dysplasia and widened lateral ventricles.

Methods: The couple have elected induced abortion after careful counseling. Skin tissue sample from the abortus and peripheral venous blood samples from both parents were collected for the extraction of genomic DNA, which was then subjected to whole exome sequencing.

Spastin Interacts with CRMP2 to Regulate Neurite Outgrowth by Controlling Microtubule Dynamics through Phosphorylation Modifications.

Aims: Our work aims to revealing the underlying microtubule mechanism of neurites outgrowth during neuronal development and also proposes a feasible intervention pathway for reconstructing neural network connections after nerve injury.

Background: Microtubule polymerization and severing form the basis for neurite outgrowth and branch formation. However, the mechanisms that underlie the dynamic instability of microtubules are unclear.

First report of the multiresistance gene cfr in Pasteurella multocida strains of avian origin from China.

Objectives: The aim of this study was to investigate the presence and genetic environment of the multiresistance gene cfr gene in Pasteurella multocida of avian origin from China.

Methods: A total of 113 P. multocida isolates were collected from sick poultries (ducks, chickens and geese) from 2003 to 2016 in Southern China and were screened for the presence of the cfr gene by PCR.

M-like protein SrM is not crucial to the virulence of a novel isolate of Streptococcus equi subsp. ruminatorum from Macaca mulatta.

In this study, a Streptococcus strainnamed FJ1804, was isolated from a blood sample collected from a dead Macaca mulatta in China and, was subsequently classified as Streptococcus equi subsp. ruminatorum (S.e.