Transcriptomic and targeted metabolomic analyses provide insights into the flavonoids biosynthesis in the flowers of Lonicera macranthoides.

Background: Flavonoids are one of the bioactive ingredients of Lonicera macranthoides (L. macranthoides), however, their biosynthesis in the flower is still unclear. In this study, combined transcriptomic and targeted metabolomic analyses were performed to clarify the flavonoids biosynthesis during flowering of L.

Molecular cytogenetic characterization and phylogenetic analysis of four species (Poaceae).

Chromosomes of four (Andersson, 1855) species including (Andersson, 1855), (Schumann & Lauterb, 1901), (Hackel, 1882) and (Chen & Renvoize, 2005) were analyzed using sequentially combined PI and DAPI (CPD) staining and fluorescence hybridization (FISH) with 45S rDNA probe. To elucidate the phylogenetic relationship among the four species, the homology of repetitive sequences among the four species was analyzed by comparative genomic hybridization (cGISH). Subsequently four species were clustered based on the internal transcribed spacer (ITS) of 45S rDNA.

Nuclear ribosomal ITS functional paralogs resolve the phylogenetic relationships of a late-Miocene radiation cycad Cycas (Cycadaceae).

Cycas is the most widespread and diverse genus among the ancient cycads, but the extant species could be the product of late Miocene rapid radiations. Taxonomic treatments to date for this genus are quite controversial, which makes it difficult to elucidate its evolutionary history. We cloned 161 genomic ITS sequences from 31 species representing all sections of Cycas.

Development of microsatellite markers in Parakmeria nitida (Magnoliaceae).

Premise Of The Study: We developed microsatellite makers for Parakmeria nitida to investigate its population structure and conservation genetics.

Methods And Results: A total of 25 microsatellite primer pairs were developed using the Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO) protocol, and polymorphism was assessed in three natural populations of P. nitida.

Isolation and characterization of microsatellite markers from Clematoclethra scandens (Actinidiaceae).

Premise Of The Study: Microsatellite makers were developed for Clematoclethra scandens to investigate its population genetics and speciation.

Methods And Results: A total of 36 microsatellite markers were isolated using the Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO) method. Their polymorphisms were assessed in two natural populations.

High nrDNA ITS polymorphism in the ancient extant seed plant Cycas: incomplete concerted evolution and the origin of pseudogenes.

Molecular studies of six species from the ancient extant seed plant Cycas, covering a wide range of its morphological diversity and all major areas of distribution, revealed a high level of intra-individual polymorphism of the internal transcribed spacer (ITS1, 5.8S, and ITS2) region, indicative of incomplete nrDNA concerted evolution. Through a range of comparisons of sequence characteristics to functional cDNA ITS copies, including sequence length and substitution variation, GC content, secondary structure stability, the presence of a conserved motif in the 5.

Genetic differentiation and relationships of populations in the Cycas balansae complex (Cycadaceae) and its conservation implications.

Background And Aims: The Cycas balansae complex is arguably a controversial group with regard to species delineation. Some taxonomists recognize a single polymorphic species while others distinguish five narrowly defined ones. The unresolved taxonomy has the potential to bring about significant problems for species conservation.

ISSR variation in the endemic and endangered plant Cycas guizhouensis (Cycadaceae).

Background And Aims: Cycas guizhouensis (Cycadaceae) is a rare and endangered species endemic to the southwest of China. An investigation was undertaken into the genetic variation of wild populations.

Methods: ISSR markers were used to determine the genetic variation within and between 12 extant populations of this species.