Simultaneous determination of seven bioactive lignans in Herpetospermum caudigerum by RP-HPLC method.

A reversed-phase high-performance liquid chromatographic method with diode array detection was established to simultaneously determine the seven bioactive lignans in Herpetospermum caudigerum, namely ent-isolariciresinol (1), dehydrodiconiferyl alcohol (2), herpetrione (3), herpetin (4), herpetetrone (5), herpetotriol (6) amd herpetal (7). The HPLC assay was performed on a Restek Pinnacle DB C18 column (250 x 4.6 mm, 5 microm) with gradient elution of acetonitrile and 0.

Preparation, characterization, and pharmacokinetic evaluation of puerarin submicron emulsion.

A novel formulation of puerarin was studied. Puerarin submicron emulsion was prepared by complex phase inversion-high-pressure homogenization technology. Characterization, distribution of drug in emulsion, short-term stability, and pharmacokinetics of emulsion were evaluated.

The study to reduce the hemolysis side effect of puerarin by a submicron emulsion delivery system.

A safe and effective delivery system with a submicron emulsion for puerarin was studied. Puerarin submicron emulsion was prepared by a novel complex-phase inversion-high press homogenization technology. The mechanism to reduce the hemolysis side effect of puerarin was studied by blood cell counts in rabbits.