Response mechanism of Saccharomyces cerevisiae under benzoic acid stress in ethanol fermentation.

Sugarcane molasses is an ideal economical raw material for ethanol production because of its wide availability, low cost and nutrient content. However, benzoic acid compounds with toxic effects on yeast cells are commonly found in sugarcane molasses. At present, the molecular mechanism of the toxic effects of benzoic acid on Saccharomyces cerevisiae has not been elucidated.

A Self-Assembling γPFD-SpyCatcher Hydrogel Scaffold for the Coimmobilization of SpyTag-Enzymes to Facilitate the Catalysis of Regulated Enzymes.

In this study, a γPFD-SpyCatcher hydrogel scaffold with the capacity for spontaneous assembly was established. With a maximum loading capacity of a 1:1 molar ratio with SpyTag-enzymes, the immobilized proteins can not only rapidly provide pure enzymes but also exhibit improved thermal and pH stability. The results of the transmission electron microscopic analysis and the traits they present indicated that SpyCatcher promotes the aggregation of γPFD and the formation of hydrogels.

Taxonomic identification, phenol biodegradation and soil remediation of the strain Rhodococcus sacchari sp. nov. Z13.

Phenols are highly toxic chemicals that are extensively used in industry and produce large amounts of emissions. Notably, phenols released into the soil are highly persistent, causing long-term harm to human health and the environment. In this study, a gram-positive, aerobic, and rod-shaped bacterial strain, Z13, with efficient phenol degradation ability, was isolated from the soil of sugarcane fields.

Fumigaclavine C ameliorates liver steatosis by attenuating hepatic de novo lipogenesis via modulation of the RhoA/ROCK signaling pathway.

Background: Non-alcoholic fatty liver disease (NAFLD) has been well defined as a common chronic liver metabolism disorder. Statins as a first-line therapeutic treatment had some side effects. Here, we found that Fumigaclavine C (FC) was collected from endophytic Aspergillus terreus via the root of Rhizophora stylosa (Rhizophoraceae), had potential anti-adipogenic and hepatoprotective effects both in vitro and in vivo without obvious adverse side effects.

Metabolome analysis of the response and tolerance mechanisms of Saccharomyces cerevisiae to formic acid stress.

Various inhibitors are produced during the hydrolysis of lignocellulosic biomass that can interfere with the growth of yeast cells and the production of bioethanol. Formic acid is a common weak acid inhibitor present in lignocellulosic hydrolysate that has toxic effects on yeast cells. However, the mechanism of the response of Saccharomyces cerevisiae to formic acid is not fully understood.

Transcriptomic analysis of formic acid stress response in Saccharomyces cerevisiae.

Formic acid is a representative small molecule acid in lignocellulosic hydrolysate that can inhibit the growth of Saccharomyces cerevisiae cells during alcohol fermentation. However, the mechanism of formic acid cytotoxicity remains largely unknown. In this study, RNA-Seq technology was used to study the response of S.

Enhanced extracellular recombinant keratinase activity in SCK6 through signal peptide optimization and site-directed mutagenesis.

Keratinase has a great commercial value owing to its applications in the enzymatic dehairing of goatskins. In this study, we adopted a combined strategy to enhance the extracellular recombinant keratinase activity in SCK6. First, nine signal peptides were screened to enhance the expression of extracellular keratinase.

Glycine betaine enhances biodegradation of phenol in high saline environments by the halophilic strain sp. PT-20.

The halophilic bacterial strain PT-20, isolated from saline alkali soil samples and identified as a member of the genus , exhibited a robust ability to degrade phenol under high salt conditions. It was determined that strain PT-20 was capable of degrading 1000 mg L phenol completely in the presence of 10% NaCl within 120 h. Under the optimal degradation conditions, pH 8.

High-expression keratinase by Bacillus subtilis SCK6 for enzymatic dehairing of goatskins.

An extracellular keratinase gene from Bacillus sp. LCB12, isolated from saline-alkali soil, was cloned and high-effectively expressed in Bacillus subtilis SCK6. The enzyme was purified by ammonium sulphate precipitation and cation-exchange chromatography.

Adaptations of HNA-14 required for long-term survival under osmotic challenge: a multi-omics perspective.

Genomic sequence, transcriptomic, metabolomic and fatty acid analyses of strain HNA-14 were performed to understand the mechanism of salt tolerance for long-term survival. The results indicated that strain HNA-14 has different osmotic resistance mechanisms for long-term survival and short-term salt stress. The cells mainly synthesized compatible solutes to resist osmotic pressure when cultured under nutrient deficient conditions, while they can slow down the synthesis rate and uptake from the environment when cultured under a nutritionally rich environment.

Streptomyces populi sp. nov., a novel endophytic actinobacterium isolated from stem of Populus adenopoda Maxim.

A novel endophytic actinobacterium strain, designated A249, was isolated from the stem of Populus adenopoda collected at Mount Qingcheng in south-west China. Its taxonomic position was determined by using a polyphasic approach. The cultural and morphological characteristics of isolate A249 were consistent with members of the genus Streptomyces.

Streptomyces geranii sp. nov., a novel endophytic actinobacterium isolated from root of Geranium carolinianum L.

A novel endophytic actinomycete, designated A301, was isolated from the root of Geranium carolinianum Linn collected from Mount Emei in China and characterized using a polyphasic approach. Growth occurred at 10-37 °C, pH 6-11 and in the presence of 0-5 % NaCl (w/v). Strain A301 contained ll-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.

Halomonas saliphila sp. nov., a moderately halophilic bacterium isolated from a saline soil.

A novel, Gram-stain-negative, aerobic, rod-shaped, motile and moderately halophilic bacterium, designated strain LCB169, was isolated from a saline soil sample from Gansu Province, PR China. The cells of LCB169 grew at 10-52 °C (optimum 30 °C), at pH 6.0-10.

Ornithinibacillus salinisoli sp. nov., a moderately halophilic bacterium isolated from a saline-alkali soil.

A taxonomic study was performed on strain LCB256, which was isolated from a saline-alkali soil sample taken from northwestern China. Cells of strain LCB256 were Gram-stain-positive, aerobic, rod-shaped and grew at 3-17 % (w/v) NaCl (optimum 10-15 %), 10-52 °C (optimum 25-30 °C) and pH 7.0-9.

Screening cyhalothrin degradation strains from locust epiphytic bacteria and studying Paracoccus acridae SCU-M53 cyhalothrin degradation process.

All locust epiphytic bacteria were screened and a total of 62 epiphytic bacteria were obtained from samples of Acrida cinerea. Via phylogenetic analysis, the 62 epiphytic bacteria were allocated to 27 genera, 18 families, 13 orders, six classes, and four phylums. Then, cyhalothrin degradation experiments were conducted, and the 10 strains that degraded more than 30% cyhalothrin and Paracoccus acridae SCU-M53 showed the highest cyhalothrin degradation rate of 70.

Planococcus salinus sp. nov., a moderately halophilic bacterium isolated from a saline-alkali soil.

A novel aerobic, Gram-stain-positive, motile, moderately halophilic and coccoid bacterial strain, designated LCB217, was isolated from a saline-alkali soil in north-western China and identified using a polyphasic taxonomic approach. Growth occurred with 3-15 % (w/v) NaCl (optimum 3-5 %), at 10-45 °C (optimum 30 °C) and at pH 7.0-9.

Microbial transformation of resveratrol by endophyte Streptomyces sp. A12 isolated from Polygonum cuspidatum.

Resveratrol (1) undergoes microbial transformation when fermented with Streptomyces sp. A12 to yield 3, 5, 4'-trimethoxy-trans-stilbene (2). The structure of the compound 2 was elucidated using the modern spectroscopic techniques.

Paracoccusacridae sp. nov., isolated from the insect Acrida cinerea living in deserted cropland.

A Gram-stain-negative, aerobic, rod-shaped, non-motile bacterial strain, designated SCU-M53T, was isolated from the insect Acrida cinerea. Phylogenetic analysis on the basis of 16S rRNA gene sequences showed that strain SCU-M53T belonged to the genus Paracoccus, having Paracoccus chinensisNBRC 104937T (97.04 % 16S rRNA gene sequence similarity) and Paracoccus niistensis KCTC 22789T (96.

Comparative analysis of chemical constituents, antimicrobial and antioxidant activities of ethylacetate extracts of Polygonum cuspidatum and its endophytic actinomycete, Streptomyces sp. A0916.

The present study investigated the chemical composition of ethylacetate extracts from an endophytic actinomycete Streptomyces sp. A0916 and its host Polygonum cuspidatum. A comparative analysis of the antimicrobial and antioxidant properties of the extracts was also conducted.

Sphingobacterium griseoflavum sp. nov., isolated from the insect Teleogryllus occipitalis living in deserted cropland.

A Gram-stain-negative, aerobic, rod-shaped, non-motile bacterial strain, designated SCU-B140T, was isolated from the insect Teleogryllus occipitalis. Phylogenetic analysis on the basis of 16S rRNA gene sequence showed that strain SCU-B140T belonged to the genus Sphingobacterium. Sphingobacterium bambusae KCTC 22814T (97.

Erwinia teleogrylli sp. nov., a Bacterial Isolate Associated with a Chinese Cricket.

A bacterial isolate (SCU-B244T) was obtained in China from crickets (Teleogryllus occipitalis) living in cropland deserted for approximately 10 years. The isolated bacteria were Gram-negative, facultatively anaerobic, oxidase-negative rods. A preliminary analysis of the 16S rRNA gene sequence indicated that the strain belongs to either the genus Erwinia or Pantoea.

Oceanobacillus damuensis sp. nov. and Oceanobacillus rekensis sp. nov., isolated from saline alkali soil samples.

Two moderately halophilic strains, PT-11(T) and PT-20(T), were isolated from saline alkali soil samples collected in Shache County, Xinjiang Province, China. Both strains are aerobic, Gram-positive, motile rods. Strain PT-11(T) grows at 15-40 °C and at pH 6.

Speciation analysis of aluminium(III) in natural waters and biological fluids by complexing with various catechols followed by differential pulse voltammetry detection.

The biological effects of aluminium have received much attention in recent years. Speciation of Al is of basic relevance as it concerns its reactivity and bioavailability. A differential pulse voltammetry (DPV) procedure is proposed for speciation analysis of Al(III) in natural waters and biological fluids using six catechols (L-dopa, dopamine, epinephrine, norepinephrine, caffeic acid and o-benzenediol) as electroactive ligands.