Binning Metagenomic Contigs Using Contig Embedding and Decomposed Tetranucleotide Frequency.

Metagenomic binning is a crucial step in metagenomic research. It can aggregate the genome sequences belonging to the same microbial species into independent bins. Most existing methods ignore the semantic information of contigs and lack effective processing of tetranucleotide frequency, resulting in insufficient and complex feature information extracted for binning and poor binning results.

Balanced Solution Versus Normal Saline in Predicted Severe Acute Pancreatitis: A Stepped Wedge Cluster Randomized Trial.

Objective: To compare the effect of balanced multielectrolyte solutions (BMESs) versus normal saline (NS) for intravenous fluid on chloride levels and clinical outcomes in patients with predicted severe acute pancreatitis (pSAP).

Background: Isotonic crystalloids are recommended for initial fluid therapy in acute pancreatitis, but whether the use of BMES in preference to NS confers clinical benefits is unknown.

Methods: In this multicenter, stepped-wedge, cluster-randomized trial, we enrolled patients with pSAP (acute physiology and chronic health evaluation II score ≥8 and C-reactive protein >150 mg/L) admitted within 72 hours of the advent of symptoms.

Effects of erythromycin on biofilm formation and resistance mutation of Escherichia coli on pristine and UV-aged polystyrene microplastics.

Microplastics (MPs) and antibiotics co-occur widely in the environment and pose combined risk to microbial communities. The present study investigated the effects of erythromycin on biofilm formation and resistance mutation of a model bacterium, E. coli, on the surface of pristine and UV-aged polystyrene (PS) MPs sized 1-2 mm.

CircSNX6 promotes proliferation, metastasis, and angiogenesis in hepatocellular carcinoma via miR-383-5p/VEGFA signaling pathway.

The role of circular RNA (circRNAs) in hepatocellular carcinoma (HCC) has been extensively studied. Previous research has highlighted the regulatory role of circSNX6 in HCC cells and tissues. However, the precise mechanism underlying HCC progression still requires comprehensive investigation.

Effects of Erythromycin on and the Intestinal Microbiome across Different Salinity Levels.

The exposure of aquatic organisms to pollutants often occurs concomitantly with salinity fluctuations. Here, we reported the effects of erythromycin (0.250, 7.

IGF2 is upregulated by its antisense RNA to potentiate pancreatic cancer progression.

Pancreatic cancer is a deadly cancer. More and more long noncoding RNAs (lncRNAs) have received confirmation to be dysregulated in tumors and exert the regulatory function. Studies have suggested that lncRNA insulin-like growth factor 2 antisense RNA (IGF2-AS) participates in the development of some cancers.

Rapid Trace Detection of Sulfite Residue in White Wine Using a Multichannel Colorimetric Nanozyme Sensor.

As a commonly used food additive, sulfite (SO) is popular with food manufacturers due to the functions of bleaching, sterilizing, and oxidation resistance. However, excess sulfites can pose a threat to human health. Therefore, it is particularly important to achieve rapid and sensitive detection of SO.

Fluorescent and smartphone imaging detection of tetracycline residues based on luminescent europium ion-functionalized the regular octahedral UiO-66-NH.

Antibiotic residues cause extensive damage to food security, thus arousing serious concerns. Hence, rapid and sensitive detection of antibiotic residues is crucial to food safety. This study aimed to propose a portable, visual, intelligent and rapid method for tetracycline detection.

Strain-Induced Structural Phase Transitions in Epitaxial (001) BiCoO Films: A First-Principles Study.

We have simulated BiCoO3 films epitaxially grown along (001) direction with density functional theory computations. Leading candidates for the lowest-energy phases have been identified. The tensile strains induce magnetic phase transition in the ground state (P4mm symmetry) from a C-type antiferromagnetic order to a G-type order for the in-plane lattice parameter above 3.

CDCA7 serves as a novel prognostic marker in human hepatocellular carcinoma.

c-Myc oncogene plays an important role in tumorigenesis, cell division cycle associated 7 (CDCA7), recently found that it is a direct target gene of c-Myc, is upregulated in many tumors, but its role in tumor progression is still poorly understood. CDCA7 expression and prognosis were analyzed in hepatocellular carcinoma using TIMER2.0 and Kaplan-Meier databases, while genomic changes were studied using cbioportal.

Phosphate and Borate-Based Composite Interface of Single-Crystal LiNiCoMnO Enables Excellent Electrochemical Stability at High Operation Voltage.

The application of nickel-rich cathodes in lithium-ion batteries has been hampered by its rapid capacity/voltage fading and limited performance of rate. In this work, a passivation technique is used to create a stable composite interface on single-crystal LiNiCoMnO (NCM811) surface, which greatly improves the cycle life-span and high-voltage constancy of cathode with 4.5 and 4.

Silencing of IGHG1 reverses the resistance of pancreatic cancer to multidrug chemotherapy by modulating autophagy.

Background: Pancreatic cancer is one of the most aggressive tumors with a high-mortality rate. First-line drugs include 5-fluorouracil (5-FU), gemcitabine (GEM), and oxaliplatin (OXA). Resistance to 5-FU, GEM, and OXA is a major challenge.

Lanthanide bimetallic MOF-based fluorescent sensor for sensitive and visual detection of sulfamerazine and malachite.

A lanthanide terbium/europium metal-organic framework (TbEu-MOF) was prepared by one-step solvothermal method at room temperature. A series of characterizations including scanning electron microscopy, powder X-ray diffraction spectra, Fourier transform infrared spectra and X-ray photoelectron spectroscopy were carried out to clarify the physical characteristics of the synthesized material. The data clarified that the prepared TbEu-MOF possessed rod-like morphology with a width of 1-2 μm, and had good crystal structure, good stability, response speed and excitation-independent emission feature.

Fluorescent Sensing of Ciprofloxacin and Chloramphenicol in Milk Samples via Inner Filter Effect and Photoinduced Electron Transfer Based on Nanosized Rod-Shaped Eu-MOF.

Rapid, facile, and accurate detection of antibiotic residues is vital for practical applications. Herein, we designed a sensitive, visual, and rapid analytical method for sensitive detection of ciprofloxacin and chloramphenicol based on a nanosized rod-shaped Europium metal organic framework (Eu-MOF). The fluorescent Eu-MOF was firstly synthesized by a simple synthetic route at room temperature, which displays a red emission.

Autonomous mobile platform for monitoring air emissions from industrial and municipal wastewater ponds.

Unlabelled: Significant amounts of volatile organic compounds and greenhouse gases are generated from wastewater lagoons and tailings ponds in Alberta, Canada. Accurate measurements of these air pollutants and greenhouse gases are needed to support management and regulatory decisions. A mobile platform was developed to measure air emissions from tailings pond in the oil sands region of Alberta.

A proton shelter inspired by the sugar coating of acidophilic archaea.

The acidophilic archaeons are a group of single-celled microorganisms that flourish in hot acid springs (usually pH < 3) but maintain their internal pH near neutral. Although there is a lack of direct evidence, the abundance of sugar modifications on the cell surface has been suggested to provide the acidophiles with protection against proton invasion. In this study, a hydroxyl (OH)-rich polymer brush layer was prepared to mimic the OH-rich sugar coating.

Solidified liquid layer model expands the application fields of quartz crystal microbalance.

The application of a quartz crystal microbalance (QCM) in liquid is hindered by the complexity of data analysis. Recently, a "solidified liquid layer" (SLL) model has been proposed to simplify the data analysis. Here, missing evidence to support the SLL model is provided: 1) the SLL model is responsive to the density change of the liquid environment, 2) thickness values from the SLL model (T(SLL) ) are in agreement with values measured by ellipsometry.

Integrated poly(dimethysiloxane) with an intrinsic nonfouling property approaching "absolute" zero background in immunoassays.

The key to achieve a highly sensitive and specific protein microarray assay is to prevent nonspecific protein adsorption to an "absolute" zero level because any signal amplification method will simultaneously amplify signal and noise. Here, we develop a novel solid supporting material, namely, polymer coated initiator integrated poly(dimethysiloxane) (iPDMS), which was able to achieve such "absolute" zero (i.e.

Real-time measurement of the mass of water expelled by poly(N-isopropylacrylamide) brushes upon thermo-induced collapse.

An impedance analysis of quartz crystal microbalance data reveals the mass of water expelled by poly(N-isopropylacrylamide) brushes upon the thermo-induced collapse.

Surface-imprinted nanostructured layer-by-layer film for molecular recognition of theophylline derivatives.

In this article we report the introduction of the cooperativity of various specific interactions combined with photo-cross-linking of the interlayers to yield binding sites that can realize better selectivity and imprinting efficiency of a surface molecularly imprinted LbL film (SMILbL), thus providing a new approach toward fabrication of nanostructured molecularly imprinted thin films. It involves preassembly of poly(acrylic acid) (PAA) conjugated of the theophylline residue template via a disulfide bridge, denoted as PAAtheo 15, in solution, and layer-by-layer (LbL) assembly of PAAtheo 15 and a positively charged photoreactive diazo resin (DAR) to form multilayer thin film with designed architecture. After photo-cross-linking of the film and template removal, binding sites specific to 7-(beta-hydroxyethyl)theophylline (Theo-ol) molecules are introduced within the film.

Study viscoelasticity of ultrathin poly(oligo(ethylene glycol) methacrylate) brushes by a quartz crystal microbalance with dissipation.

Ultrathin polymer brushes play important roles in natural and artificial systems. To better understand and utilize their unique behaviors, characterization is a fundamental, but not trivial, task. In this paper, we demonstrated that the quartz crystal microbalance with dissipation (QCM-D) could be applied to study ultrathin poly(oligo(ethylene glycol) methacrylate) brushes.

Peak VO(2) is more potent than B-type natriuretic peptide as a prognostic parameter in cardiac patients.

Background: It is well-known that both B-type natriuretic peptide (BNP) and peak oxygen uptake (VO(2)) are independent predictors of mortality in patients with heart failure. This study investigates the predictive power of BNP and peak VO(2) for survival in cardiac patients.

Methods And Results: A total of 609 patients with cardiac disease participated in the study.

High diastolic blood pressure during exercise is associated with hypercholesterolemia in patients with coronary artery disease.

Evaluating blood pressure response during exercise rather than during rest might better detect a subtle impairment in relaxation of the resistance vessel in hypercholesterolemia. We examined the relation between serum cholesterol and blood pressure response during exercise in patients with coronary artery disease. One hundred and forty-eight consecutive patients with coronary artery disease were monitored during symptom-limited incremental exercise testing with a cycle ergometer.