Dobinin K Displays Antiplasmodial Activity through Disruption of Mitochondria and Generation of Reactive Oxygen Species.

Dobinin K is a novel eudesmane sesquiterpenoids compound isolated from the root of and displays potential antiplasmodial activity in vivo. Here, we evaluate the antiplasmodial activity of dobinin K in vitro and study its acting mechanism. The antiplasmodial activity of dobinin K in vitro was evaluated by concentration-, time-dependent, and stage-specific parasite inhibition assay.

Identification and functional characterization of REGULATORY PARTICLE NON-ATPASE 1a-like (AhRPN1a-like) in peanuts during aluminum-induced programmed cell death.

The toxicity of aluminum (Al) in acidic soil is a prevalent problem and causes reduced crop yields. In the plant response to Al toxicity, programmed cell death (PCD) appears to be one of the important mechanisms. However, the regulation of Al-induced PCD remains poorly understood.

Identification and functional characterization of the xyloglucan endotransglucosylase/hydrolase 32 (AhXTH32) in peanut during aluminum-induced programmed cell death.

The toxicity of aluminum (Al) in acidic soil is a prevalent problem and causes reduced crop yields. In the plant response to Al toxicity, programmed cell death (PCD) appears to be an important mechanism. The plant cell wall of crop roots is the predominant site targeted by Al.

[Detection of Porphyromonas gingivalis rag genotypes in patients of chronic periodontitis with chronic obstructive pulmonary disease].

Purpose: To investigate the distribution of Porphyromonas gingivalis(P.g) rag genotypes in patients of chronic periodontitis with chronic obstructive pulmonary disease (COPD).

Methods: Thirty patients with chronic periodontitis and 30 patients with chronic periodontitis complicated with COPD were included.

Genome-wide identification, evolutionary and expression analyses of LEA gene family in peanut (Arachis hypogaea L.).

Background: Late embryogenesis abundant (LEA) proteins are a group of highly hydrophilic glycine-rich proteins, which accumulate in the late stage of seed maturation and are associated with many abiotic stresses. However, few peanut LEA genes had been reported, and the research on the number, location, structure, molecular phylogeny and expression of AhLEAs was very limited.

Results: In this study, 126 LEA genes were identified in the peanut genome through genome-wide analysis and were further divided into eight groups.

Genome-wide identification and evolutionary analysis of RLKs involved in the response to aluminium stress in peanut.

Background: As an important cash crop, the yield of peanut is influenced by soil acidification and pathogen infection. Receptor-like protein kinases play important roles in plant growth, development and stress responses. However, little is known about the number, location, structure, molecular phylogeny, and expression of RLKs in peanut, and no comprehensive analysis of RLKs in the Al stress response in peanuts have been reported.

Transcriptome analysis reveals significant difference in gene expression and pathways between two peanut cultivars under Al stress.

Aluminum (Al) toxicity is an important factor in limiting peanut growth on acidic soil. The molecular mechanisms underlying peanut responses to Al stress are largely unknown. In this study, we performed transcriptome analysis of the root tips (0-1 cm) of peanut cultivar ZH2 (Al-sensitive) and 99-1507 (Al-tolerant) respectively.

Crosstalk between melatonin and nitric oxide in plant development and stress responses.

Melatonin is widely involved in plant growth and stress responses as a master regulator. Melatonin treatment alters the levels of endogenous nitric oxide (NO) and NO affects endogenous melatonin content. Melatonin and NO may induce various plant physiological behavior through interaction mechanism.

Nitric oxide is a suppressor of aluminum-induced mitochondria and caspase-like protease-dependent programmed cell death in plants.

Aluminum (Al) promotes programmed cell death (PCD) in plants. Although a lot of knowledge about the mechanisms of Al tolerance has been learned, how Al-induced PCD is regulated by nitric oxide (NO) is poorly understood. Mitochondrion is the regulatory center for PCD.

Nitric oxide acts as an antioxidant and inhibits programmed cell death induced by aluminum in the root tips of peanut (Arachis hypogaea L.).

Aluminum (Al) causes programmed cell death (PCD) in plants. Our previous studies have confirmed that nitric oxide (NO) inhibits Al-induced PCD in the root tips of peanut. However, the mechanism by which NO inhibits Al-induced PCD is unclear.

Genome-wide analysis of the MATE gene family in potato.

The multidrug and toxic compound extrusion (MATE) protein family is a newly discovered family of secondary transporters that extrude metabolic waste and a variety of antibiotics out of the cell using an electrochemical gradient of H or Na across the membrane. The main function of MATE gene family is to participate in the process of plant detoxification and morphogenesis. The genome-wide analysis of the MATE genes in potato genome was conducted.

Role of nitric oxide and hydrogen sulfide in plant aluminum tolerance.

As gasotransmitter, nitric oxide (NO) and hydrogen sulfide (HS) are involved in the regulation of plant tolerance to abiotic stresses. Aluminum (Al) toxicity triggers synthesis of NO and HS and seriously affects plant growth and productivity. Exogenous NO and HS alleviate Al toxicity in plants.

The central role of hydrogen sulfide in plant responses to toxic metal stress.

With the increase of industrial wastes, sewage irrigation, chemical fertilizers and pesticides, metal contamination is increasingly serious. How to reduce the environmental risk has become a compelling problem in cultivated land. As a gaseous signal molecule, hydrogen sulfide (HS) is involved in multiple plant responses to toxic metal stress.

Nitric oxide suppresses aluminum-induced programmed cell death in peanut (Arachis hypoganea L.) root tips by improving mitochondrial physiological properties.

Aluminum (Al) stress alters nitric oxide (NO) and induces programmed cell death (PCD) in plants. Recent study has shown that NO inhibits Al-induced PCD. However, the mechanism of NO inhibiting Al-induced PCD has not been revealed yet.

Analysis of Mitochondrial Markers of Programmed Cell Death.

Mitochondria play a crucial role in programmed cell death (PCD) in plants. In most cases of mitochondria-dependent PCD, cytochrome c (Cyt c) released from mitochondria due to the opening of mitochondrial permeability transition pore (MPTP) and the activation of caspase-like proteases. Here we describe the analytic methods of mitochondrial markers of PCD including mitochondria isolation, mitochondrial membrane permeability, mitochondrial inner membrane potential, Cytc release, ATP, and mitochondrial reactive oxygen species (ROS).

Nitric Oxide Inhibits Al-Induced Programmed Cell Death in Root Tips of Peanut ( L.) by Affecting Physiological Properties of Antioxidants Systems and Cell Wall.

It has been reported that nitric oxide (NO) is a negative regulator of aluminum (Al)-induced programmed cell death (PCD) in peanut root tips. However, the inhibiting mechanism of NO on Al-induced PCD is unclear. In order to investigate the mechanism by which NO inhibits Al-induced PCD, the effects of co-treatment Al with the exogenous NO donor or the NO-specific scavenger on peanut root tips, the physiological properties of antioxidants systems and cell wall (CW) in root tip cells of NO inhibiting Al-induced PCD were studied with two peanut cultivars.

Regulation of gaseous signaling molecules on proline metabolism in plants.

Proline accumulation plays an important role in the response and adaptation of plants to abiotic stress. Gaseous signaling molecules such as nitric oxide (NO), carbon monoxide (CO), and hydrogen sulfide (HS) are involved in complicated events of cell signaling. However, the regulatory mechanisms of gaseous signaling molecules on proline synthesis and degradation are still unclear.

Nitric oxide inhibits aluminum-induced programmed cell death in peanut (Arachis hypoganea L.) root tips.

It had been reported that Aluminum (Al) stress altered nitric oxide (NO) concentration and induced programmed cell death (PCD) in plants. However, the relationship between NO and PCD occurrence under Al stress is unclear. The results showed that cell death induced by Al was significant negative correlation with the inhibition of Al on root elongation growth in peanut.

[Preparation and luminescent properties of light scattering fluorescent resin].

With the methods of compound-melting and high-temperature molding, PC/YAG:Ce fluorescent resin pieces were prepared. The transmittance of the prepared PC/YAG:Ce fluorescent resin in 500~800 nm reaches approximately 65% (0.71 mm thick, double sides polished).

Aluminum induces rapidly mitochondria-dependent programmed cell death in Al-sensitive peanut root tips.

Background: Although many studies suggested that aluminum (Al) induced programmed cell death (PCD) in plants, the mechanism of Al-induced PCD and its effects in Al tolerance is limited. This study was to investigate the mechanism and type of Al induced PCD and the relationship between PCD and Al tolerance.

Results: In this study, two genotypes of peanut 99-1507 (Al tolerant) and ZH2 (Al sensitive) were used to investigate Al-induced PCD.

Mitochondrial alterations during Al-induced PCD in peanut root tips.

Previous study found there was a negative relationship between Al-induced PCD and Al-resistance in peanut. The present research was undertaken to verify whether mitochondria play a significant role in PCD induced by Al in peanut. The roots of Al-tolerant plants were found to exhibit more intensive root growth, while accumulating less Al³⁺ than Al-sensitive plants under Al treatment.

[Preparation and optical properties of MgAl2O4/Ce:YAG transparent ceramics].

High-purity ultrafine MgAl2O4 powder was synthesized by metal-alkoxide method and calcining for 2-4 h. And then MgAl2O4/Ce:YAG transparent ceramics were fabricated by hot-pressed sintering and hot isostatic pressed sintering technique with YAG:Ce powder and MgAl2O4 powder. The transparent ceramics were characterized by XRD, SEM, EDS and fluorescence spectrometer, respectively.

Aluminum-induced programmed cell death promoted by AhSAG, a senescence-associated gene in Arachis hypoganea L.

Programmed cell death (PCD) is a foundational cellular process in plant development and elimination of damaged cells under environmental stresses. In this study, Al induced PCD in two peanut (Arachis hypoganea L.) cultivars Zhonghua 2 (Al-sensitive) and 99-1507 (Al-tolerant) using DNA ladder, TUNEL detection and electron microscopy.

Nitric oxide improves aluminum tolerance by regulating hormonal equilibrium in the root apices of rye and wheat.

Nitric oxide (NO) has emerged as a key molecule involved in many physiological processes in plants. Whether NO reduces aluminum (Al) toxicity by regulating the levels of endogenous hormones in plants is still unknown. In this study, the effects of NO on Al tolerance and hormonal changes in the root apices of rye and wheat were investigated.

[Effects of sodium nitroprusside on mitochondrial function of rye and wheat root tip under aluminum stress].

Sodium nitroprusside (SNP) could ameliorate the inhibition effect of Al on root growth of rye (Secale cereale L. cv. King) and wheat (Triticum aestivum L.